Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms

Abstract Salmonella Typhimurium and its monophasic variant S. 4,[5],12:i:- are the dominant serotypes associated with pigs in many countries. We investigated their population structure on nine farms using whole genome sequencing, and their genotypic and phenotypic variation. The population structure...

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Auteurs principaux:	Eleonora Tassinari, Geraldine Duffy, Matt Bawn, Catherine M. Burgess, Evonne M. McCabe, Peadar G. Lawlor, Gillian Gardiner, Robert A. Kingsley
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2019
Sujets:	Medicine R Science Q
Accès en ligne:	https://doaj.org/article/61801dce9bbb4e12852b0c734e708d5a
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spelling	oai:doaj.org-article:61801dce9bbb4e12852b0c734e708d5a2021-12-02T15:08:46ZMicroevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms10.1038/s41598-019-45216-w2045-2322https://doaj.org/article/61801dce9bbb4e12852b0c734e708d5a2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-45216-whttps://doaj.org/toc/2045-2322Abstract Salmonella Typhimurium and its monophasic variant S. 4,[5],12:i:- are the dominant serotypes associated with pigs in many countries. We investigated their population structure on nine farms using whole genome sequencing, and their genotypic and phenotypic variation. The population structure revealed the presence of phylogenetically distinct clades consisting of closely related clones of S. Typhimurium or S. 4,[5],12:i:- on each pig farm, that persisted between production cycles. All the S. 4,[5],12:i:- strains carried the Salmonella genomic island-4 (SGI-4), which confers resistance to heavy metals, and half of the strains contained the mTmV prophage, harbouring the sopE virulence gene. Most clonal groups were highly drug resistant due to the presence of multiple antimicrobial resistance (AMR) genes, and two clades exhibited evidence of recent on-farm plasmid-mediated acquisition of additional AMR genes, including an IncHI2 plasmid. Biofilm formation was highly variable but had a strong phylogenetic signature. Strains capable of forming biofilm with the greatest biomass were from the S. 4,[5],12:i:- and S. Typhimurium DT104 clades, the two dominant pandemic clones found over the last 25 years. On-farm microevolution resulted in enhanced biofilm formation in subsequent production cycle.Eleonora TassinariGeraldine DuffyMatt BawnCatherine M. BurgessEvonne M. McCabePeadar G. LawlorGillian GardinerRobert A. KingsleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eleonora Tassinari Geraldine Duffy Matt Bawn Catherine M. Burgess Evonne M. McCabe Peadar G. Lawlor Gillian Gardiner Robert A. Kingsley Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms
description	Abstract Salmonella Typhimurium and its monophasic variant S. 4,[5],12:i:- are the dominant serotypes associated with pigs in many countries. We investigated their population structure on nine farms using whole genome sequencing, and their genotypic and phenotypic variation. The population structure revealed the presence of phylogenetically distinct clades consisting of closely related clones of S. Typhimurium or S. 4,[5],12:i:- on each pig farm, that persisted between production cycles. All the S. 4,[5],12:i:- strains carried the Salmonella genomic island-4 (SGI-4), which confers resistance to heavy metals, and half of the strains contained the mTmV prophage, harbouring the sopE virulence gene. Most clonal groups were highly drug resistant due to the presence of multiple antimicrobial resistance (AMR) genes, and two clades exhibited evidence of recent on-farm plasmid-mediated acquisition of additional AMR genes, including an IncHI2 plasmid. Biofilm formation was highly variable but had a strong phylogenetic signature. Strains capable of forming biofilm with the greatest biomass were from the S. 4,[5],12:i:- and S. Typhimurium DT104 clades, the two dominant pandemic clones found over the last 25 years. On-farm microevolution resulted in enhanced biofilm formation in subsequent production cycle.
format	article
author	Eleonora Tassinari Geraldine Duffy Matt Bawn Catherine M. Burgess Evonne M. McCabe Peadar G. Lawlor Gillian Gardiner Robert A. Kingsley
author_facet	Eleonora Tassinari Geraldine Duffy Matt Bawn Catherine M. Burgess Evonne M. McCabe Peadar G. Lawlor Gillian Gardiner Robert A. Kingsley
author_sort	Eleonora Tassinari
title	Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms
title_short	Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms
title_full	Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms
title_fullStr	Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms
title_full_unstemmed	Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms
title_sort	microevolution of antimicrobial resistance and biofilm formation of salmonella typhimurium during persistence on pig farms
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/61801dce9bbb4e12852b0c734e708d5a
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Microevolution of antimicrobial resistance and biofilm formation of Salmonella Typhimurium during persistence on pig farms

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