Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications

Herein, we report the green synthesis of silver nanoparticles (OE-Ag NPs) by ecofriendly green processes using biological molecules of <i>Olea europaea</i> leaf extract. Green synthesized OE-Ag NPs were successfully characterized using different spectroscopic techniques. Antibacterial ac...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hanen Sellami, Shakeel Ahmad Khan, Ishaq Ahmad, Abdullah A. Alarfaj, Abdurahman H. Hirad, Ahmed E. Al-Sabri
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Ag NPs <i>Olea europaea</i> green synthesis antioxidant antimicrobial cytotoxic Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/618536c1148144318cfdf9cb030969a8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:618536c1148144318cfdf9cb030969a8
record_format	dspace
spelling	oai:doaj.org-article:618536c1148144318cfdf9cb030969a82021-11-25T17:57:49ZGreen Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications10.3390/ijms2222125621422-00671661-6596https://doaj.org/article/618536c1148144318cfdf9cb030969a82021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12562https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Herein, we report the green synthesis of silver nanoparticles (OE-Ag NPs) by ecofriendly green processes using biological molecules of <i>Olea europaea</i> leaf extract. Green synthesized OE-Ag NPs were successfully characterized using different spectroscopic techniques. Antibacterial activity of OE-Ag NPs was assessed against four different bacteriological strains using the dilution serial method. The cytotoxic potential was determined against MCF-7 carcinoma cells using MTT assay in terms of cell viability percentage. Antioxidant properties were evaluated in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. Biocompatibility was further examined by incubating the synthesized NPs with hMSC cells for 24 h. The results were demonstrated that synthesized OE-Ag NPs presented excellent log<sub>10</sub> reduction in the growth of all the tested bacterial strains, which as statistically equivalent (<i>p</i> > 0.05) to the standard antibiotic drug. Moreover, they also demonstrated excellent cytotoxic efficacy against the MCF-7 carcinoma cells compared to plant lead extract and Com-Ag NPs. Green synthesized OE-Ag NPs appeared more biocompatible to hMSC and 293T cells compared to Com-Ag NPs. Excellent biological results of the OE-Ag NPs might be attributed to the synergetic effect of NPs’ properties and the adsorbed secondary metabolites of plant leaf extract. Hence, this study suggests that synthesized OE-Ag NPs can be a potential contender for their various biological and nutraceutical applications. Moreover, this study will open a new avenue to produce biocompatible nanoparticles with additional biological functionalities from the plants.Hanen SellamiShakeel Ahmad KhanIshaq AhmadAbdullah A. AlarfajAbdurahman H. HiradAhmed E. Al-SabriMDPI AGarticleAg NPs<i>Olea europaea</i>green synthesisantioxidantantimicrobialcytotoxicBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12562, p 12562 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ag NPs <i>Olea europaea</i> green synthesis antioxidant antimicrobial cytotoxic Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	Ag NPs <i>Olea europaea</i> green synthesis antioxidant antimicrobial cytotoxic Biology (General) QH301-705.5 Chemistry QD1-999 Hanen Sellami Shakeel Ahmad Khan Ishaq Ahmad Abdullah A. Alarfaj Abdurahman H. Hirad Ahmed E. Al-Sabri Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications
description	Herein, we report the green synthesis of silver nanoparticles (OE-Ag NPs) by ecofriendly green processes using biological molecules of <i>Olea europaea</i> leaf extract. Green synthesized OE-Ag NPs were successfully characterized using different spectroscopic techniques. Antibacterial activity of OE-Ag NPs was assessed against four different bacteriological strains using the dilution serial method. The cytotoxic potential was determined against MCF-7 carcinoma cells using MTT assay in terms of cell viability percentage. Antioxidant properties were evaluated in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. Biocompatibility was further examined by incubating the synthesized NPs with hMSC cells for 24 h. The results were demonstrated that synthesized OE-Ag NPs presented excellent log<sub>10</sub> reduction in the growth of all the tested bacterial strains, which as statistically equivalent (<i>p</i> > 0.05) to the standard antibiotic drug. Moreover, they also demonstrated excellent cytotoxic efficacy against the MCF-7 carcinoma cells compared to plant lead extract and Com-Ag NPs. Green synthesized OE-Ag NPs appeared more biocompatible to hMSC and 293T cells compared to Com-Ag NPs. Excellent biological results of the OE-Ag NPs might be attributed to the synergetic effect of NPs’ properties and the adsorbed secondary metabolites of plant leaf extract. Hence, this study suggests that synthesized OE-Ag NPs can be a potential contender for their various biological and nutraceutical applications. Moreover, this study will open a new avenue to produce biocompatible nanoparticles with additional biological functionalities from the plants.
format	article
author	Hanen Sellami Shakeel Ahmad Khan Ishaq Ahmad Abdullah A. Alarfaj Abdurahman H. Hirad Ahmed E. Al-Sabri
author_facet	Hanen Sellami Shakeel Ahmad Khan Ishaq Ahmad Abdullah A. Alarfaj Abdurahman H. Hirad Ahmed E. Al-Sabri
author_sort	Hanen Sellami
title	Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications
title_short	Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications
title_full	Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications
title_fullStr	Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications
title_full_unstemmed	Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications
title_sort	green synthesis of silver nanoparticles using <i>olea europaea</i> leaf extract for their enhanced antibacterial, antioxidant, cytotoxic and biocompatibility applications
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/618536c1148144318cfdf9cb030969a8
work_keys_str_mv	AT hanensellami greensynthesisofsilvernanoparticlesusingioleaeuropaeaileafextractfortheirenhancedantibacterialantioxidantcytotoxicandbiocompatibilityapplications AT shakeelahmadkhan greensynthesisofsilvernanoparticlesusingioleaeuropaeaileafextractfortheirenhancedantibacterialantioxidantcytotoxicandbiocompatibilityapplications AT ishaqahmad greensynthesisofsilvernanoparticlesusingioleaeuropaeaileafextractfortheirenhancedantibacterialantioxidantcytotoxicandbiocompatibilityapplications AT abdullahaalarfaj greensynthesisofsilvernanoparticlesusingioleaeuropaeaileafextractfortheirenhancedantibacterialantioxidantcytotoxicandbiocompatibilityapplications AT abdurahmanhhirad greensynthesisofsilvernanoparticlesusingioleaeuropaeaileafextractfortheirenhancedantibacterialantioxidantcytotoxicandbiocompatibilityapplications AT ahmedealsabri greensynthesisofsilvernanoparticlesusingioleaeuropaeaileafextractfortheirenhancedantibacterialantioxidantcytotoxicandbiocompatibilityapplications
_version_	1718411805732110336

Green Synthesis of Silver Nanoparticles Using <i>Olea europaea</i> Leaf Extract for Their Enhanced Antibacterial, Antioxidant, Cytotoxic and Biocompatibility Applications

Ejemplares similares