Sampling insulin in different tissue compartments using microdialysis: methodological aspects

Abstract Sampling the concentration of insulin in human skin using microdialysis is challenging because of low intracutaneous concentrations and low recovery, presumably due to adsorption of insulin to the microdialysis system. In this study, we aimed to (1) measure how the concentration of insulin...

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Autores principales:	Alexandra Högstedt, Bijar Ghafouri, Erik Tesselaar, Simon Farnebo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61865f1d290142818012404bbcfa675f
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spelling	oai:doaj.org-article:61865f1d290142818012404bbcfa675f2021-12-02T11:57:57ZSampling insulin in different tissue compartments using microdialysis: methodological aspects10.1038/s41598-020-78728-x2045-2322https://doaj.org/article/61865f1d290142818012404bbcfa675f2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78728-xhttps://doaj.org/toc/2045-2322Abstract Sampling the concentration of insulin in human skin using microdialysis is challenging because of low intracutaneous concentrations and low recovery, presumably due to adsorption of insulin to the microdialysis system. In this study, we aimed to (1) measure how the concentration of insulin varies in three different tissue compartments (intracutaneous, subcutaneous and intravenous) and (2) to study how much insulin is adsorbed to the microdialysis catheter membranes and tubing during a typical microdialysis experiment, both in vivo and in vitro. We hypothesized that (1) the concentration of insulin decreases from the intravenous compartment to the intracutaneous and subcutaneous tissue, and that (2) adsorption of insulin to the microdialysis membrane and tubing impairs the recovery of insulin from the tissue. In this experimental study, microdialysis catheters were inserted intracutaneously, subcutaneously and intravenously in 11 healthy subjects. Systemic endogenous hyperinsulinemia was induced by intake of an oral glucose load. Insulin concentration was measured in the dialysate and in the extracted samples from the catheter membrane and tubings. In vitro microdialysis was performed to investigate the temporal resolution of the adsorption. After an oral glucose load insulin concentration increased intravenously, but not in the intracutaneous or subcutaneous compartments, while glucose, lactate and pyruvate concentrations increased in all compartments. The adsorption of insulin to the microdialysis membrane in vivo was highest in the intravenous compartment (p = 0.01), compared to the intracutaneous and subcutaneous compartments. In vitro, the adsorption to the microdialysis membrane was highest one hour after sampling, then the concentration gradually decreased after three and five hours of sampling. The concentration of insulin in peripheral tissues is low, probably due to decreasing tissue vascularity. Adsorption of insulin to the microdialysis membrane is modest but time-dependent. This finding highlights the importance of a stabilization time for the microdialysis system before sampling tissue analytes.Alexandra HögstedtBijar GhafouriErik TesselaarSimon FarneboNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra Högstedt Bijar Ghafouri Erik Tesselaar Simon Farnebo Sampling insulin in different tissue compartments using microdialysis: methodological aspects
description	Abstract Sampling the concentration of insulin in human skin using microdialysis is challenging because of low intracutaneous concentrations and low recovery, presumably due to adsorption of insulin to the microdialysis system. In this study, we aimed to (1) measure how the concentration of insulin varies in three different tissue compartments (intracutaneous, subcutaneous and intravenous) and (2) to study how much insulin is adsorbed to the microdialysis catheter membranes and tubing during a typical microdialysis experiment, both in vivo and in vitro. We hypothesized that (1) the concentration of insulin decreases from the intravenous compartment to the intracutaneous and subcutaneous tissue, and that (2) adsorption of insulin to the microdialysis membrane and tubing impairs the recovery of insulin from the tissue. In this experimental study, microdialysis catheters were inserted intracutaneously, subcutaneously and intravenously in 11 healthy subjects. Systemic endogenous hyperinsulinemia was induced by intake of an oral glucose load. Insulin concentration was measured in the dialysate and in the extracted samples from the catheter membrane and tubings. In vitro microdialysis was performed to investigate the temporal resolution of the adsorption. After an oral glucose load insulin concentration increased intravenously, but not in the intracutaneous or subcutaneous compartments, while glucose, lactate and pyruvate concentrations increased in all compartments. The adsorption of insulin to the microdialysis membrane in vivo was highest in the intravenous compartment (p = 0.01), compared to the intracutaneous and subcutaneous compartments. In vitro, the adsorption to the microdialysis membrane was highest one hour after sampling, then the concentration gradually decreased after three and five hours of sampling. The concentration of insulin in peripheral tissues is low, probably due to decreasing tissue vascularity. Adsorption of insulin to the microdialysis membrane is modest but time-dependent. This finding highlights the importance of a stabilization time for the microdialysis system before sampling tissue analytes.
format	article
author	Alexandra Högstedt Bijar Ghafouri Erik Tesselaar Simon Farnebo
author_facet	Alexandra Högstedt Bijar Ghafouri Erik Tesselaar Simon Farnebo
author_sort	Alexandra Högstedt
title	Sampling insulin in different tissue compartments using microdialysis: methodological aspects
title_short	Sampling insulin in different tissue compartments using microdialysis: methodological aspects
title_full	Sampling insulin in different tissue compartments using microdialysis: methodological aspects
title_fullStr	Sampling insulin in different tissue compartments using microdialysis: methodological aspects
title_full_unstemmed	Sampling insulin in different tissue compartments using microdialysis: methodological aspects
title_sort	sampling insulin in different tissue compartments using microdialysis: methodological aspects
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/61865f1d290142818012404bbcfa675f
work_keys_str_mv	AT alexandrahogstedt samplinginsulinindifferenttissuecompartmentsusingmicrodialysismethodologicalaspects AT bijarghafouri samplinginsulinindifferenttissuecompartmentsusingmicrodialysismethodologicalaspects AT eriktesselaar samplinginsulinindifferenttissuecompartmentsusingmicrodialysismethodologicalaspects AT simonfarnebo samplinginsulinindifferenttissuecompartmentsusingmicrodialysismethodologicalaspects
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Sampling insulin in different tissue compartments using microdialysis: methodological aspects

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