Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism

Abstract Antimicrobial resistance has become one of the major threats to human health. Therefore, there is a strong need for novel antimicrobials with new mechanisms of action. The kingdom of fungi is an excellent source of antimicrobials for this purpose because it encompasses countless fungal spec...

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Autores principales:	Xudong Ouyang, Jelmer Hoeksma, Gisela van der Velden, Wouter A. G. Beenker, Maria H. van Triest, Boudewijn M. T. Burgering, Jeroen den Hertog
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/618b19370c2c428c8cd86060b3f11d40
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spelling	oai:doaj.org-article:618b19370c2c428c8cd86060b3f11d402021-12-02T17:26:49ZBerkchaetoazaphilone B has antimicrobial activity and affects energy metabolism10.1038/s41598-021-98252-w2045-2322https://doaj.org/article/618b19370c2c428c8cd86060b3f11d402021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98252-whttps://doaj.org/toc/2045-2322Abstract Antimicrobial resistance has become one of the major threats to human health. Therefore, there is a strong need for novel antimicrobials with new mechanisms of action. The kingdom of fungi is an excellent source of antimicrobials for this purpose because it encompasses countless fungal species that harbor unusual metabolic pathways. Previously, we have established a library of secondary metabolites from 10,207 strains of fungi. Here, we screened for antimicrobial activity of the library against seven pathogenic bacterial strains and investigated the identity of the active compounds using ethyl acetate extraction, activity-directed purification using HPLC fractionation and chemical analyses. We initially found 280 antimicrobial strains and subsequently identified 17 structurally distinct compounds from 26 strains upon further analysis. All but one of these compounds, berkchaetoazaphilone B (BAB), were known to have antimicrobial activity. Here, we studied the antimicrobial properties of BAB, and found that BAB affected energy metabolism in both prokaryotic and eukaryotic cells. We conclude that fungi are a rich source of chemically diverse secondary metabolites with antimicrobial activity.Xudong OuyangJelmer HoeksmaGisela van der VeldenWouter A. G. BeenkerMaria H. van TriestBoudewijn M. T. BurgeringJeroen den HertogNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xudong Ouyang Jelmer Hoeksma Gisela van der Velden Wouter A. G. Beenker Maria H. van Triest Boudewijn M. T. Burgering Jeroen den Hertog Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
description	Abstract Antimicrobial resistance has become one of the major threats to human health. Therefore, there is a strong need for novel antimicrobials with new mechanisms of action. The kingdom of fungi is an excellent source of antimicrobials for this purpose because it encompasses countless fungal species that harbor unusual metabolic pathways. Previously, we have established a library of secondary metabolites from 10,207 strains of fungi. Here, we screened for antimicrobial activity of the library against seven pathogenic bacterial strains and investigated the identity of the active compounds using ethyl acetate extraction, activity-directed purification using HPLC fractionation and chemical analyses. We initially found 280 antimicrobial strains and subsequently identified 17 structurally distinct compounds from 26 strains upon further analysis. All but one of these compounds, berkchaetoazaphilone B (BAB), were known to have antimicrobial activity. Here, we studied the antimicrobial properties of BAB, and found that BAB affected energy metabolism in both prokaryotic and eukaryotic cells. We conclude that fungi are a rich source of chemically diverse secondary metabolites with antimicrobial activity.
format	article
author	Xudong Ouyang Jelmer Hoeksma Gisela van der Velden Wouter A. G. Beenker Maria H. van Triest Boudewijn M. T. Burgering Jeroen den Hertog
author_facet	Xudong Ouyang Jelmer Hoeksma Gisela van der Velden Wouter A. G. Beenker Maria H. van Triest Boudewijn M. T. Burgering Jeroen den Hertog
author_sort	Xudong Ouyang
title	Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
title_short	Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
title_full	Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
title_fullStr	Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
title_full_unstemmed	Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism
title_sort	berkchaetoazaphilone b has antimicrobial activity and affects energy metabolism
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/618b19370c2c428c8cd86060b3f11d40
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Berkchaetoazaphilone B has antimicrobial activity and affects energy metabolism

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