Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study

Abstract Background Information regarding the influence of cytoplasmic events during fertilisation on the clinical outcome remains limited. The cytoplasmic halo is one of these events. A previous study that used time-lapse technology found an association of the presence and morphokinetics of the cyt...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kenji Ezoe, Tetsuya Miki, Tadashi Okimura, Kazuo Uchiyama, Akiko Yabuuchi, Tamotsu Kobayashi, Keiichi Kato
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Cleaved embryo transfer Cytoplasmic halo In vitro fertilisation Intracytoplasmic sperm injection Live birth Pronucleus Gynecology and obstetrics RG1-991 Reproduction QH471-489
Acceso en línea:	https://doaj.org/article/61985a4a82f446baa620f3c4ae3a891f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:61985a4a82f446baa620f3c4ae3a891f
record_format	dspace
spelling	oai:doaj.org-article:61985a4a82f446baa620f3c4ae3a891f2021-11-28T12:37:47ZCharacteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study10.1186/s12958-021-00859-11477-7827https://doaj.org/article/61985a4a82f446baa620f3c4ae3a891f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12958-021-00859-1https://doaj.org/toc/1477-7827Abstract Background Information regarding the influence of cytoplasmic events during fertilisation on the clinical outcome remains limited. The cytoplasmic halo is one of these events. A previous study that used time-lapse technology found an association of the presence and morphokinetics of the cytoplasmic halo with cleavage patterns, development to the blastocyst stage, and the ongoing pregnancy rate after blastocyst transfer. Therefore, the cytoplasmic halo may be a useful predictor of the pregnancy outcome after cleaved embryo transfer. This study evaluated the ability of the cytoplasmic halo to predict a live birth after fresh cleaved embryo transfer on day 2, and sought to identify factors potentially influencing the presence and morphokinetics of the halo. Methods A total of 902 embryos cultured in the EmbryoScope+® time-lapse system and subjected to single fresh cleaved embryo transfer were retrospectively analysed. The presence and duration of a cytoplasmic halo were annotated. The initial positions of the pronuclei were also observed. The correlation between the cytoplasmic halo and live birth was evaluated and the association of the cytoplasmic halo with patient, cycle, and embryonic characteristics was determined. Results Absence of a cytoplasmic halo was associated with a significant decrease in the likelihood of a live birth after fresh cleaved embryo transfer. Prolongation of the halo, especially the duration of central repositioning of cytoplasmic granules, had an adverse impact on the live birth rate. The characteristics of the cytoplasmic halo were not affected by the ovarian stimulation method used, female age, the serum steroid hormone level on the day of trigger, or semen quality. However, the cytoplasmic halo was significantly affected by male age, oocyte diameter, and the initial position of the male pronucleus. Conclusions Absence or prolongation of the cytoplasmic halo was negatively correlated with the live birth rate after fresh cleaved embryo transfer. The characteristics of the cytoplasmic halo were strongly associated with oocyte diameter, male age, and the initial position of the male pronucleus. These findings indicate that the characteristics of the cytoplasmic halo can be used to select more competent embryos for transfer at the cleavage stage.Kenji EzoeTetsuya MikiTadashi OkimuraKazuo UchiyamaAkiko YabuuchiTamotsu KobayashiKeiichi KatoBMCarticleCleaved embryo transferCytoplasmic haloIn vitro fertilisationIntracytoplasmic sperm injectionLive birthPronucleusGynecology and obstetricsRG1-991ReproductionQH471-489ENReproductive Biology and Endocrinology, Vol 19, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cleaved embryo transfer Cytoplasmic halo In vitro fertilisation Intracytoplasmic sperm injection Live birth Pronucleus Gynecology and obstetrics RG1-991 Reproduction QH471-489
spellingShingle	Cleaved embryo transfer Cytoplasmic halo In vitro fertilisation Intracytoplasmic sperm injection Live birth Pronucleus Gynecology and obstetrics RG1-991 Reproduction QH471-489 Kenji Ezoe Tetsuya Miki Tadashi Okimura Kazuo Uchiyama Akiko Yabuuchi Tamotsu Kobayashi Keiichi Kato Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study
description	Abstract Background Information regarding the influence of cytoplasmic events during fertilisation on the clinical outcome remains limited. The cytoplasmic halo is one of these events. A previous study that used time-lapse technology found an association of the presence and morphokinetics of the cytoplasmic halo with cleavage patterns, development to the blastocyst stage, and the ongoing pregnancy rate after blastocyst transfer. Therefore, the cytoplasmic halo may be a useful predictor of the pregnancy outcome after cleaved embryo transfer. This study evaluated the ability of the cytoplasmic halo to predict a live birth after fresh cleaved embryo transfer on day 2, and sought to identify factors potentially influencing the presence and morphokinetics of the halo. Methods A total of 902 embryos cultured in the EmbryoScope+® time-lapse system and subjected to single fresh cleaved embryo transfer were retrospectively analysed. The presence and duration of a cytoplasmic halo were annotated. The initial positions of the pronuclei were also observed. The correlation between the cytoplasmic halo and live birth was evaluated and the association of the cytoplasmic halo with patient, cycle, and embryonic characteristics was determined. Results Absence of a cytoplasmic halo was associated with a significant decrease in the likelihood of a live birth after fresh cleaved embryo transfer. Prolongation of the halo, especially the duration of central repositioning of cytoplasmic granules, had an adverse impact on the live birth rate. The characteristics of the cytoplasmic halo were not affected by the ovarian stimulation method used, female age, the serum steroid hormone level on the day of trigger, or semen quality. However, the cytoplasmic halo was significantly affected by male age, oocyte diameter, and the initial position of the male pronucleus. Conclusions Absence or prolongation of the cytoplasmic halo was negatively correlated with the live birth rate after fresh cleaved embryo transfer. The characteristics of the cytoplasmic halo were strongly associated with oocyte diameter, male age, and the initial position of the male pronucleus. These findings indicate that the characteristics of the cytoplasmic halo can be used to select more competent embryos for transfer at the cleavage stage.
format	article
author	Kenji Ezoe Tetsuya Miki Tadashi Okimura Kazuo Uchiyama Akiko Yabuuchi Tamotsu Kobayashi Keiichi Kato
author_facet	Kenji Ezoe Tetsuya Miki Tadashi Okimura Kazuo Uchiyama Akiko Yabuuchi Tamotsu Kobayashi Keiichi Kato
author_sort	Kenji Ezoe
title	Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study
title_short	Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study
title_full	Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study
title_fullStr	Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study
title_full_unstemmed	Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study
title_sort	characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study
publisher	BMC
publishDate	2021
url	https://doaj.org/article/61985a4a82f446baa620f3c4ae3a891f
work_keys_str_mv	AT kenjiezoe characteristicsofthecytoplasmichaloduringfertilisationcorrelatewiththelivebirthrateafterfreshcleavedembryotransferonday2inminimalovarianstimulationcyclesaretrospectiveobservationalstudy AT tetsuyamiki characteristicsofthecytoplasmichaloduringfertilisationcorrelatewiththelivebirthrateafterfreshcleavedembryotransferonday2inminimalovarianstimulationcyclesaretrospectiveobservationalstudy AT tadashiokimura characteristicsofthecytoplasmichaloduringfertilisationcorrelatewiththelivebirthrateafterfreshcleavedembryotransferonday2inminimalovarianstimulationcyclesaretrospectiveobservationalstudy AT kazuouchiyama characteristicsofthecytoplasmichaloduringfertilisationcorrelatewiththelivebirthrateafterfreshcleavedembryotransferonday2inminimalovarianstimulationcyclesaretrospectiveobservationalstudy AT akikoyabuuchi characteristicsofthecytoplasmichaloduringfertilisationcorrelatewiththelivebirthrateafterfreshcleavedembryotransferonday2inminimalovarianstimulationcyclesaretrospectiveobservationalstudy AT tamotsukobayashi characteristicsofthecytoplasmichaloduringfertilisationcorrelatewiththelivebirthrateafterfreshcleavedembryotransferonday2inminimalovarianstimulationcyclesaretrospectiveobservationalstudy AT keiichikato characteristicsofthecytoplasmichaloduringfertilisationcorrelatewiththelivebirthrateafterfreshcleavedembryotransferonday2inminimalovarianstimulationcyclesaretrospectiveobservationalstudy
_version_	1718407893079818240

Characteristics of the cytoplasmic halo during fertilisation correlate with the live birth rate after fresh cleaved embryo transfer on day 2 in minimal ovarian stimulation cycles: a retrospective observational study

Ejemplares similares