State of rat colon microbiocenosis in chronic restraint stress treated with Selank

It is currently accepted that stress significantly affects composition of microbiocenosis due to changes in permeability of intestinal barrier and pro-inflammatory effects. This, in turn, changes behavioral reactions, anxiety and stress response. In this regard, it seems promising to use regulatory...

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Autores principales:	A. Yu. Mukhina, O. A. Medvedeva, M. V. Svishcheva, A. V. Shevchenko, N. N. Efremova, I. I. Bobyntsev, P. V. Kalutsky, L. A. Andreeva, N. F. Myasoedov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	selank microbiocenosis restraint stress microbiota dysbiosis Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/61987cdc141b4008a19b44a5e88c61f0
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spelling	oai:doaj.org-article:61987cdc141b4008a19b44a5e88c61f02021-11-22T07:09:53ZState of rat colon microbiocenosis in chronic restraint stress treated with Selank2220-76192313-739810.15789/2220-7619-2019-5-6-805-810https://doaj.org/article/61987cdc141b4008a19b44a5e88c61f02020-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1155https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398It is currently accepted that stress significantly affects composition of microbiocenosis due to changes in permeability of intestinal barrier and pro-inflammatory effects. This, in turn, changes behavioral reactions, anxiety and stress response. In this regard, it seems promising to use regulatory peptide-based neurotropic drugs including Selank to correct stress-induced dysbiosis. Our study was aimed at assessing state of rat colon microbiocenosis in modelled chronic restraint stress and treated with Selankby using 65 Wistar male rats divided into five groups (per 13 rats in each): group 1 — rats injected with saline; group 2 — injected with saline and induced chronic restraint stress; group 3–5 — administered with Selank at dose of 80 μg/kg, 250 μg/kg and 750 μg/kg body weight, respectively, and induced chronic restraint stress. Quantitative and qualitative study of animal colon microbiota was carried out according to the method by L.I. Kafarskaja and V.M. Korshunov. Identification of microorganisms was carried out by using a Maldi Biotyper Microflex mass spectrometer (Bruker, United States). Microbial species-specific composition was presented as lg CFU/g mass of examined sample. For each identified microbial genus, the relative mean and frequency of occurrence were calculated. Statistical significance of differences in mean values was determined by using Student’s t-test. Chronic restraint stress in the experiment did not result in affecting dominant microbiota species in rat colon nor reduce their frequency, however, it significantly influenced examined parameters for commensal microbiota disturbing pattern of pathogenic bacterial strains. Use of Selank led to the reversing changes in composition of colonic microbiocenosis caused by stress model. Moreover, magnitude of parameters examined in experiment after applying Selank at dose of 750 μg/kg reached those in non-stressed animals. Thus, effects related to Selank administration may presumably be mediated due to both central and peripheral effects including immunotropic and anti-inflammatory activities which contributed to restoring colon microbiocenosis composition in stress model.A. Yu. MukhinaO. A. MedvedevaM. V. SvishchevaA. V. ShevchenkoN. N. EfremovaI. I. BobyntsevP. V. KalutskyL. A. AndreevaN. F. MyasoedovSankt-Peterburg : NIIÈM imeni Pasteraarticleselankmicrobiocenosisrestraint stressmicrobiotadysbiosisInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 9, Iss 5-6, Pp 805-810 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	selank microbiocenosis restraint stress microbiota dysbiosis Infectious and parasitic diseases RC109-216
spellingShingle	selank microbiocenosis restraint stress microbiota dysbiosis Infectious and parasitic diseases RC109-216 A. Yu. Mukhina O. A. Medvedeva M. V. Svishcheva A. V. Shevchenko N. N. Efremova I. I. Bobyntsev P. V. Kalutsky L. A. Andreeva N. F. Myasoedov State of rat colon microbiocenosis in chronic restraint stress treated with Selank
description	It is currently accepted that stress significantly affects composition of microbiocenosis due to changes in permeability of intestinal barrier and pro-inflammatory effects. This, in turn, changes behavioral reactions, anxiety and stress response. In this regard, it seems promising to use regulatory peptide-based neurotropic drugs including Selank to correct stress-induced dysbiosis. Our study was aimed at assessing state of rat colon microbiocenosis in modelled chronic restraint stress and treated with Selankby using 65 Wistar male rats divided into five groups (per 13 rats in each): group 1 — rats injected with saline; group 2 — injected with saline and induced chronic restraint stress; group 3–5 — administered with Selank at dose of 80 μg/kg, 250 μg/kg and 750 μg/kg body weight, respectively, and induced chronic restraint stress. Quantitative and qualitative study of animal colon microbiota was carried out according to the method by L.I. Kafarskaja and V.M. Korshunov. Identification of microorganisms was carried out by using a Maldi Biotyper Microflex mass spectrometer (Bruker, United States). Microbial species-specific composition was presented as lg CFU/g mass of examined sample. For each identified microbial genus, the relative mean and frequency of occurrence were calculated. Statistical significance of differences in mean values was determined by using Student’s t-test. Chronic restraint stress in the experiment did not result in affecting dominant microbiota species in rat colon nor reduce their frequency, however, it significantly influenced examined parameters for commensal microbiota disturbing pattern of pathogenic bacterial strains. Use of Selank led to the reversing changes in composition of colonic microbiocenosis caused by stress model. Moreover, magnitude of parameters examined in experiment after applying Selank at dose of 750 μg/kg reached those in non-stressed animals. Thus, effects related to Selank administration may presumably be mediated due to both central and peripheral effects including immunotropic and anti-inflammatory activities which contributed to restoring colon microbiocenosis composition in stress model.
format	article
author	A. Yu. Mukhina O. A. Medvedeva M. V. Svishcheva A. V. Shevchenko N. N. Efremova I. I. Bobyntsev P. V. Kalutsky L. A. Andreeva N. F. Myasoedov
author_facet	A. Yu. Mukhina O. A. Medvedeva M. V. Svishcheva A. V. Shevchenko N. N. Efremova I. I. Bobyntsev P. V. Kalutsky L. A. Andreeva N. F. Myasoedov
author_sort	A. Yu. Mukhina
title	State of rat colon microbiocenosis in chronic restraint stress treated with Selank
title_short	State of rat colon microbiocenosis in chronic restraint stress treated with Selank
title_full	State of rat colon microbiocenosis in chronic restraint stress treated with Selank
title_fullStr	State of rat colon microbiocenosis in chronic restraint stress treated with Selank
title_full_unstemmed	State of rat colon microbiocenosis in chronic restraint stress treated with Selank
title_sort	state of rat colon microbiocenosis in chronic restraint stress treated with selank
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/61987cdc141b4008a19b44a5e88c61f0
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State of rat colon microbiocenosis in chronic restraint stress treated with Selank

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