Integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis
Abstract The association between the metabolic profile and inflammatory cytokines in psoriasis is poorly understood. We analyzed the metabolic and cytokine/chemokine profiles in serum and skin from patients with new-onset psoriasis and healthy subjects (n = 7/group) by HR-MAS NMR and Bio-Plex immuno...
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Nature Portfolio
2021
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oai:doaj.org-article:619b4b7df2d9492c9cd5cde668f6da942021-12-02T15:45:27ZIntegrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis10.1038/s41598-021-89925-72045-2322https://doaj.org/article/619b4b7df2d9492c9cd5cde668f6da942021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89925-7https://doaj.org/toc/2045-2322Abstract The association between the metabolic profile and inflammatory cytokines in psoriasis is poorly understood. We analyzed the metabolic and cytokine/chemokine profiles in serum and skin from patients with new-onset psoriasis and healthy subjects (n = 7/group) by HR-MAS NMR and Bio-Plex immunoassay. Immuno-metabolic correlation matrix was analyzed in skin and serum to identify a potential immune-metabolic signature. Metabolomics analysis showed a significant increase in ascorbate and a decrease in scyllo-inositol, and a trend towards an increase in eight other metabolites in psoriatic skin. In serum, there was a significant increase of dimethylglycine and isoleucine. In parallel, psoriatic skin exhibited an increase of early inflammatory cytokines (IL-6, IL-8, TNF-α, IL-1β) and correlation analysis highlighted some major clusters of immune-metabolic correlations. A cluster comprising scyllo-inositol and lysine showed correlations with T-cell cytokines; a cluster comprising serine and taurine showed a negative correlation with early inflammatory cytokines (IL-6, G-CSF, CCL3). A strong positive correlation was enlightened between glutathione and inflammatory cytokines/angiogenesis promoters of psoriasis. The integration of metabolic and immune data indicated a molecular signature constituted by IL-6, IL1-ra, DMG, CCL4, Ile, Gly and IL-8, which could discriminate patients and healthy subjects and could represent a candidate tool in the diagnosis of new-onset psoriasis.Elisabetta TarentiniGiulia OdoriciValeria RighiAlessia PaganelliLuca GiacomelliValentina MirisolaAdele MucciLuisa BenassiElisabetta D’AversaClaudia LasagniShaniko KaleciEva RealiCristina MagnoniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) |
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Medicine R Science Q Elisabetta Tarentini Giulia Odorici Valeria Righi Alessia Paganelli Luca Giacomelli Valentina Mirisola Adele Mucci Luisa Benassi Elisabetta D’Aversa Claudia Lasagni Shaniko Kaleci Eva Reali Cristina Magnoni Integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis |
| description |
Abstract The association between the metabolic profile and inflammatory cytokines in psoriasis is poorly understood. We analyzed the metabolic and cytokine/chemokine profiles in serum and skin from patients with new-onset psoriasis and healthy subjects (n = 7/group) by HR-MAS NMR and Bio-Plex immunoassay. Immuno-metabolic correlation matrix was analyzed in skin and serum to identify a potential immune-metabolic signature. Metabolomics analysis showed a significant increase in ascorbate and a decrease in scyllo-inositol, and a trend towards an increase in eight other metabolites in psoriatic skin. In serum, there was a significant increase of dimethylglycine and isoleucine. In parallel, psoriatic skin exhibited an increase of early inflammatory cytokines (IL-6, IL-8, TNF-α, IL-1β) and correlation analysis highlighted some major clusters of immune-metabolic correlations. A cluster comprising scyllo-inositol and lysine showed correlations with T-cell cytokines; a cluster comprising serine and taurine showed a negative correlation with early inflammatory cytokines (IL-6, G-CSF, CCL3). A strong positive correlation was enlightened between glutathione and inflammatory cytokines/angiogenesis promoters of psoriasis. The integration of metabolic and immune data indicated a molecular signature constituted by IL-6, IL1-ra, DMG, CCL4, Ile, Gly and IL-8, which could discriminate patients and healthy subjects and could represent a candidate tool in the diagnosis of new-onset psoriasis. |
| format |
article |
| author |
Elisabetta Tarentini Giulia Odorici Valeria Righi Alessia Paganelli Luca Giacomelli Valentina Mirisola Adele Mucci Luisa Benassi Elisabetta D’Aversa Claudia Lasagni Shaniko Kaleci Eva Reali Cristina Magnoni |
| author_facet |
Elisabetta Tarentini Giulia Odorici Valeria Righi Alessia Paganelli Luca Giacomelli Valentina Mirisola Adele Mucci Luisa Benassi Elisabetta D’Aversa Claudia Lasagni Shaniko Kaleci Eva Reali Cristina Magnoni |
| author_sort |
Elisabetta Tarentini |
| title |
Integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis |
| title_short |
Integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis |
| title_full |
Integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis |
| title_fullStr |
Integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis |
| title_full_unstemmed |
Integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis |
| title_sort |
integrated metabolomic analysis and cytokine profiling define clusters of immuno-metabolic correlation in new-onset psoriasis |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/619b4b7df2d9492c9cd5cde668f6da94 |
| work_keys_str_mv |
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