A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application

Corynebacterium glutamicum is one of the important industrial microorganisms for production of amino acids and other value-added compounds. Most expression vectors used in C. glutamicum are based on inducible promoter (Ptac or Ptrc) activated by isopropyl-β-D-thiogalactopyranoside (IPTG). However, t...

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Autores principales:	Nan Lu, Chenglin Zhang, Wenjie Zhang, Haoran Xu, Yuhong Li, Minhua Wei, Jing Meng, Yan Meng, Junzhe Wang, Ning Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Corynebacterium glutamicum expression vector IolR iolT1 myo-inositol 5-aminolevulinic acid Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/61a0778e804744e5b5e251ca538f57d3
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id	oai:doaj.org-article:61a0778e804744e5b5e251ca538f57d3
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spelling	oai:doaj.org-article:61a0778e804744e5b5e251ca538f57d32021-11-15T05:41:15ZA Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application2296-418510.3389/fbioe.2021.746322https://doaj.org/article/61a0778e804744e5b5e251ca538f57d32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.746322/fullhttps://doaj.org/toc/2296-4185Corynebacterium glutamicum is one of the important industrial microorganisms for production of amino acids and other value-added compounds. Most expression vectors used in C. glutamicum are based on inducible promoter (Ptac or Ptrc) activated by isopropyl-β-D-thiogalactopyranoside (IPTG). However, these vectors seem unsuitable for large-scale industrial production due to the high cost and toxicity of IPTG. Myo-inositol is an ideal inducer because of its non-toxicity and lower price. In this study, a myo-inositol-inducible expression vector pMI-4, derived from the expression vector pXMJ19, was constructed. Besides the original chloramphenicol resistance gene cat, multiple cloning sites, and rrnB terminator, the pMI-4 (6,643 bp) contains the iolRq cassette and the myo-inositol-inducible promoter PiolT1. The pMI-4 could stably replicate in the C. glutamicum host. Meanwhile, the non-myo-inositol degradation host strain C. glutamicumΔiolGΔoxiCΔoxiDΔoxiE for maintaining the pMI-4 was developed. Overexpression of hemAM and hemL using pMI-4 resulted in a significant accumulation of 5-aminolevulinic acid, indicating its potential application in metabolic engineering and industrial fermentation.Nan LuChenglin ZhangWenjie ZhangHaoran XuYuhong LiMinhua WeiJing MengYan MengJunzhe WangNing ChenFrontiers Media S.A.articleCorynebacterium glutamicumexpression vectorIolRiolT1myo-inositol5-aminolevulinic acidBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Corynebacterium glutamicum expression vector IolR iolT1 myo-inositol 5-aminolevulinic acid Biotechnology TP248.13-248.65
spellingShingle	Corynebacterium glutamicum expression vector IolR iolT1 myo-inositol 5-aminolevulinic acid Biotechnology TP248.13-248.65 Nan Lu Chenglin Zhang Wenjie Zhang Haoran Xu Yuhong Li Minhua Wei Jing Meng Yan Meng Junzhe Wang Ning Chen A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
description	Corynebacterium glutamicum is one of the important industrial microorganisms for production of amino acids and other value-added compounds. Most expression vectors used in C. glutamicum are based on inducible promoter (Ptac or Ptrc) activated by isopropyl-β-D-thiogalactopyranoside (IPTG). However, these vectors seem unsuitable for large-scale industrial production due to the high cost and toxicity of IPTG. Myo-inositol is an ideal inducer because of its non-toxicity and lower price. In this study, a myo-inositol-inducible expression vector pMI-4, derived from the expression vector pXMJ19, was constructed. Besides the original chloramphenicol resistance gene cat, multiple cloning sites, and rrnB terminator, the pMI-4 (6,643 bp) contains the iolRq cassette and the myo-inositol-inducible promoter PiolT1. The pMI-4 could stably replicate in the C. glutamicum host. Meanwhile, the non-myo-inositol degradation host strain C. glutamicumΔiolGΔoxiCΔoxiDΔoxiE for maintaining the pMI-4 was developed. Overexpression of hemAM and hemL using pMI-4 resulted in a significant accumulation of 5-aminolevulinic acid, indicating its potential application in metabolic engineering and industrial fermentation.
format	article
author	Nan Lu Chenglin Zhang Wenjie Zhang Haoran Xu Yuhong Li Minhua Wei Jing Meng Yan Meng Junzhe Wang Ning Chen
author_facet	Nan Lu Chenglin Zhang Wenjie Zhang Haoran Xu Yuhong Li Minhua Wei Jing Meng Yan Meng Junzhe Wang Ning Chen
author_sort	Nan Lu
title	A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
title_short	A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
title_full	A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
title_fullStr	A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
title_full_unstemmed	A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application
title_sort	myo-inositol-inducible expression system for corynebacterium glutamicum and its application
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/61a0778e804744e5b5e251ca538f57d3
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A Myo-Inositol-Inducible Expression System for Corynebacterium glutamicum and Its Application

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