A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity

Abstract Paternal obesity is known to have a negative impact on the male’s reproductive health as well as the health of his offspring. Although epigenetic mechanisms have been implicated in the non-genetic transmission of acquired traits, the effect of paternal obesity on gene expression in the prei...

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Autores principales:	Laura Bernhardt, Marcus Dittrich, Rabih El-Merahbi, Antoine-Emmanuel Saliba, Tobias Müller, Grzegorz Sumara, Jörg Vogel, Stefanie Nichols-Burns, Megan Mitchell, Thomas Haaf, Nady El Hajj
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61b018e0b75f4c008fc73cf8722871ee
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spelling	oai:doaj.org-article:61b018e0b75f4c008fc73cf8722871ee2021-12-02T15:23:28ZA genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity10.1038/s41598-021-81226-32045-2322https://doaj.org/article/61b018e0b75f4c008fc73cf8722871ee2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81226-3https://doaj.org/toc/2045-2322Abstract Paternal obesity is known to have a negative impact on the male’s reproductive health as well as the health of his offspring. Although epigenetic mechanisms have been implicated in the non-genetic transmission of acquired traits, the effect of paternal obesity on gene expression in the preimplantation embryo has not been fully studied. To this end, we investigated whether paternal obesity is associated with gene expression changes in eight-cell stage embryos fathered by males on a high-fat diet. We used single embryo RNA-seq to compare the gene expression profile of embryos generated by males on a high fat (HFD) versus control (CD) diet. This analysis revealed significant upregulation of the Samd4b and Gata6 gene in embryos in response to a paternal HFD. Furthermore, we could show a significant increase in expression of both Gata6 and Samd4b during differentiation of stromal vascular cells into mature adipocytes. These findings suggest that paternal obesity may induce changes in the male germ cells which are associated with the gene expression changes in the resulting preimplantation embryos.Laura BernhardtMarcus DittrichRabih El-MerahbiAntoine-Emmanuel SalibaTobias MüllerGrzegorz SumaraJörg VogelStefanie Nichols-BurnsMegan MitchellThomas HaafNady El HajjNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laura Bernhardt Marcus Dittrich Rabih El-Merahbi Antoine-Emmanuel Saliba Tobias Müller Grzegorz Sumara Jörg Vogel Stefanie Nichols-Burns Megan Mitchell Thomas Haaf Nady El Hajj A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
description	Abstract Paternal obesity is known to have a negative impact on the male’s reproductive health as well as the health of his offspring. Although epigenetic mechanisms have been implicated in the non-genetic transmission of acquired traits, the effect of paternal obesity on gene expression in the preimplantation embryo has not been fully studied. To this end, we investigated whether paternal obesity is associated with gene expression changes in eight-cell stage embryos fathered by males on a high-fat diet. We used single embryo RNA-seq to compare the gene expression profile of embryos generated by males on a high fat (HFD) versus control (CD) diet. This analysis revealed significant upregulation of the Samd4b and Gata6 gene in embryos in response to a paternal HFD. Furthermore, we could show a significant increase in expression of both Gata6 and Samd4b during differentiation of stromal vascular cells into mature adipocytes. These findings suggest that paternal obesity may induce changes in the male germ cells which are associated with the gene expression changes in the resulting preimplantation embryos.
format	article
author	Laura Bernhardt Marcus Dittrich Rabih El-Merahbi Antoine-Emmanuel Saliba Tobias Müller Grzegorz Sumara Jörg Vogel Stefanie Nichols-Burns Megan Mitchell Thomas Haaf Nady El Hajj
author_facet	Laura Bernhardt Marcus Dittrich Rabih El-Merahbi Antoine-Emmanuel Saliba Tobias Müller Grzegorz Sumara Jörg Vogel Stefanie Nichols-Burns Megan Mitchell Thomas Haaf Nady El Hajj
author_sort	Laura Bernhardt
title	A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
title_short	A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
title_full	A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
title_fullStr	A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
title_full_unstemmed	A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
title_sort	genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/61b018e0b75f4c008fc73cf8722871ee
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A genome-wide transcriptomic analysis of embryos fathered by obese males in a murine model of diet-induced obesity

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