Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala

Newcastle disease (ND) is a pandemic viral disease of poultry. It is highly contagious and causes high morbidity and mortality in affected flocks. The disease is caused by Avian orthoavulavirus 1, commonly known as Newcastle disease virus (NDV) belongs to the family Paramyxoviridae. The virus affec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	U. Rashi, M. Mini, P. M. Priya, Surya Sankar, K. Vijayakumar
Formato:	article
Lenguaje:	EN
Publicado:	Director of Academics and Research, Kerala Veterinary and Animal Sciences University 2021
Materias:	haemagglutination test intracerebral pathogenicity index mean death time Animal biochemistry QP501-801 Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/61b30cb4ad004e1bbe741b954c3771cb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:61b30cb4ad004e1bbe741b954c3771cb
record_format	dspace
spelling	oai:doaj.org-article:61b30cb4ad004e1bbe741b954c3771cb2021-11-15T13:31:42ZIsolation and pathotyping of Newcastle disease virus isolated from birds in Kerala10.51966/jvas.2021.52.3.245-2490971-07012582-0605https://doaj.org/article/61b30cb4ad004e1bbe741b954c3771cb2021-07-01T00:00:00Zhttps://www.jvas.in/public_html/upload/article_file/article_file_r0qihw.pdf?t=r0qihwhttps://doaj.org/toc/0971-0701https://doaj.org/toc/2582-0605Newcastle disease (ND) is a pandemic viral disease of poultry. It is highly contagious and causes high morbidity and mortality in affected flocks. The disease is caused by Avian orthoavulavirus 1, commonly known as Newcastle disease virus (NDV) belongs to the family Paramyxoviridae. The virus affects almost 241 species of birds. Based on the pathogenicity, the virus is classified into five pathotypes viz., viscerotropic velogenic, neurotropic velogenic mesogenic, lentogenic and asymptomatic enteric NDV. The severity of the disease varies with the viral pathotype. Isolation and identification along with pathotyping of the virus provides a basis for understanding the type of virus circulating in the region. In the present study, tissue samples from dead/ ailing birds showing lesions/clinical signs suggestive of ND were collected. They were subjected to virus isolation in embryonated chicken eggs and identified by haemagglutination test and confirmed by haemagglutination inhibition test. Eight NDV isolates were obtained out of 55 tissue samples and were classified into pathotypes by intracerebral pathogenicity index (ICPI) and mean death time (MDT). The ICPI values varied from 0.75 to 1.53 and MDT from 54 h. to 79.2 h. Out of eight isolates, three belonged to velogenic group and five were of mesogenic pathotype. The study revealed the circulation of virulent NDV in Kerala. The pathogenicity tests provide a basis for understanding the epidemiology of NDU. RashiM. MiniP. M. PriyaSurya SankarK. VijayakumarDirector of Academics and Research, Kerala Veterinary and Animal Sciences Universityarticlehaemagglutination testintracerebral pathogenicity indexmean death timeAnimal biochemistryQP501-801Science (General)Q1-390ENJournal of Veterinary and Animal Sciences, Vol 52, Iss 3, Pp 245-249 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	haemagglutination test intracerebral pathogenicity index mean death time Animal biochemistry QP501-801 Science (General) Q1-390
spellingShingle	haemagglutination test intracerebral pathogenicity index mean death time Animal biochemistry QP501-801 Science (General) Q1-390 U. Rashi M. Mini P. M. Priya Surya Sankar K. Vijayakumar Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala
description	Newcastle disease (ND) is a pandemic viral disease of poultry. It is highly contagious and causes high morbidity and mortality in affected flocks. The disease is caused by Avian orthoavulavirus 1, commonly known as Newcastle disease virus (NDV) belongs to the family Paramyxoviridae. The virus affects almost 241 species of birds. Based on the pathogenicity, the virus is classified into five pathotypes viz., viscerotropic velogenic, neurotropic velogenic mesogenic, lentogenic and asymptomatic enteric NDV. The severity of the disease varies with the viral pathotype. Isolation and identification along with pathotyping of the virus provides a basis for understanding the type of virus circulating in the region. In the present study, tissue samples from dead/ ailing birds showing lesions/clinical signs suggestive of ND were collected. They were subjected to virus isolation in embryonated chicken eggs and identified by haemagglutination test and confirmed by haemagglutination inhibition test. Eight NDV isolates were obtained out of 55 tissue samples and were classified into pathotypes by intracerebral pathogenicity index (ICPI) and mean death time (MDT). The ICPI values varied from 0.75 to 1.53 and MDT from 54 h. to 79.2 h. Out of eight isolates, three belonged to velogenic group and five were of mesogenic pathotype. The study revealed the circulation of virulent NDV in Kerala. The pathogenicity tests provide a basis for understanding the epidemiology of ND
format	article
author	U. Rashi M. Mini P. M. Priya Surya Sankar K. Vijayakumar
author_facet	U. Rashi M. Mini P. M. Priya Surya Sankar K. Vijayakumar
author_sort	U. Rashi
title	Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala
title_short	Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala
title_full	Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala
title_fullStr	Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala
title_full_unstemmed	Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala
title_sort	isolation and pathotyping of newcastle disease virus isolated from birds in kerala
publisher	Director of Academics and Research, Kerala Veterinary and Animal Sciences University
publishDate	2021
url	https://doaj.org/article/61b30cb4ad004e1bbe741b954c3771cb
work_keys_str_mv	AT urashi isolationandpathotypingofnewcastlediseasevirusisolatedfrombirdsinkerala AT mmini isolationandpathotypingofnewcastlediseasevirusisolatedfrombirdsinkerala AT pmpriya isolationandpathotypingofnewcastlediseasevirusisolatedfrombirdsinkerala AT suryasankar isolationandpathotypingofnewcastlediseasevirusisolatedfrombirdsinkerala AT kvijayakumar isolationandpathotypingofnewcastlediseasevirusisolatedfrombirdsinkerala
_version_	1718428437891252224

Isolation and pathotyping of Newcastle disease virus isolated from birds in Kerala

Ejemplares similares