Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer

ABSTRACT The spread of colistin resistance gene mcr-1 at the animal-human interface remains largely unknown. This work aimed to investigate the molecular characteristics of two extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains with mcr-1, i.e., strains H8 and H9, isolated from...

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Autores principales:	Beiwen Zheng, Hao Xu, Chen Huang, Xiao Yu, Lihua Guo, Huiming Han, Jing Zhang, Xiawei Jiang, Chunlei Chen, Yonghong Xiao
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	farmer MCR-1.12 ESBLs PacBio coexistence mink Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/61b8ad024db34833a5fadf1591058357
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spelling	oai:doaj.org-article:61b8ad024db34833a5fadf15910583572021-11-15T15:22:24ZOccurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer10.1128/mSphere.00602-192379-5042https://doaj.org/article/61b8ad024db34833a5fadf15910583572019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00602-19https://doaj.org/toc/2379-5042ABSTRACT The spread of colistin resistance gene mcr-1 at the animal-human interface remains largely unknown. This work aimed to investigate the molecular characteristics of two extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains with mcr-1, i.e., strains H8 and H9, isolated from the same mink farmer. In this study, five mcr-positive E. coli strains were isolated from the mink farm. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) identified two genetically unrelated MCR-1 producers (H8 and H9) from the same farmer and two clonally related MCR-1-positive isolates (M5 and M6) from two different mink samples. Additionally, a mcr-1 variant, designated mcr-1.12, was identified in isolate M4. MIC determination revealed that all of the MCR-producing strains exhibited multiresistant phenotypes but showed susceptibility to imipenem, meropenem, amikacin, and tigecycline. Replicon typing showed that mcr-1 was associated with IncHI2 plasmids in 4 cases, while the gene was located on an IncI2 plasmid in 1 case. PacBio sequencing and plasmid analysis confirmed that the mcr-1 gene was located on an ∼204-kb IncHI2 plasmid in H8 and was carried by an ∼61-kb IncI2 plasmid in H9. To our knowledge, this work represents the first report of the occurrence of MCR-producing isolates from mink. Moreover, our report also describes the coexistence of two different MCR-1 producers in the same farmer. It highlights that fur farms can be reservoirs of mcr-1 genes. The identification of mcr-carrying plasmids on a fur farm is of potential public health importance, as it suggests that mcr is widespread in the animal husbandry industry. IMPORTANCE Colistin resistance is a real threat for both human and animal health. The mobile colistin resistance gene mcr has contributed to the persistence and transmission of colistin resistance at the interfaces of animals, humans, and ecosystems. Although mcr genes have usually been recovered from food animals, patients, and healthy humans, transmission of mcr genes at the animal-human interface remains largely unknown. This was the first study to isolate and characterize MCR-producing isolates from mink, as well as to report the coexistence of two different MCR-1 producers in the same farmer. The characterization and analysis of two MCR-1-producing E. coli isolates may have important implications for comprehension of the transmission dynamics of these bacteria. We emphasize the importance of improved multisectorial surveillance of colistin-resistant E. coli in this region.Beiwen ZhengHao XuChen HuangXiao YuLihua GuoHuiming HanJing ZhangXiawei JiangChunlei ChenYonghong XiaoAmerican Society for MicrobiologyarticlefarmerMCR-1.12ESBLsPacBiocoexistenceminkMicrobiologyQR1-502ENmSphere, Vol 4, Iss 6 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	farmer MCR-1.12 ESBLs PacBio coexistence mink Microbiology QR1-502
spellingShingle	farmer MCR-1.12 ESBLs PacBio coexistence mink Microbiology QR1-502 Beiwen Zheng Hao Xu Chen Huang Xiao Yu Lihua Guo Huiming Han Jing Zhang Xiawei Jiang Chunlei Chen Yonghong Xiao Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer
description	ABSTRACT The spread of colistin resistance gene mcr-1 at the animal-human interface remains largely unknown. This work aimed to investigate the molecular characteristics of two extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains with mcr-1, i.e., strains H8 and H9, isolated from the same mink farmer. In this study, five mcr-positive E. coli strains were isolated from the mink farm. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) identified two genetically unrelated MCR-1 producers (H8 and H9) from the same farmer and two clonally related MCR-1-positive isolates (M5 and M6) from two different mink samples. Additionally, a mcr-1 variant, designated mcr-1.12, was identified in isolate M4. MIC determination revealed that all of the MCR-producing strains exhibited multiresistant phenotypes but showed susceptibility to imipenem, meropenem, amikacin, and tigecycline. Replicon typing showed that mcr-1 was associated with IncHI2 plasmids in 4 cases, while the gene was located on an IncI2 plasmid in 1 case. PacBio sequencing and plasmid analysis confirmed that the mcr-1 gene was located on an ∼204-kb IncHI2 plasmid in H8 and was carried by an ∼61-kb IncI2 plasmid in H9. To our knowledge, this work represents the first report of the occurrence of MCR-producing isolates from mink. Moreover, our report also describes the coexistence of two different MCR-1 producers in the same farmer. It highlights that fur farms can be reservoirs of mcr-1 genes. The identification of mcr-carrying plasmids on a fur farm is of potential public health importance, as it suggests that mcr is widespread in the animal husbandry industry. IMPORTANCE Colistin resistance is a real threat for both human and animal health. The mobile colistin resistance gene mcr has contributed to the persistence and transmission of colistin resistance at the interfaces of animals, humans, and ecosystems. Although mcr genes have usually been recovered from food animals, patients, and healthy humans, transmission of mcr genes at the animal-human interface remains largely unknown. This was the first study to isolate and characterize MCR-producing isolates from mink, as well as to report the coexistence of two different MCR-1 producers in the same farmer. The characterization and analysis of two MCR-1-producing E. coli isolates may have important implications for comprehension of the transmission dynamics of these bacteria. We emphasize the importance of improved multisectorial surveillance of colistin-resistant E. coli in this region.
format	article
author	Beiwen Zheng Hao Xu Chen Huang Xiao Yu Lihua Guo Huiming Han Jing Zhang Xiawei Jiang Chunlei Chen Yonghong Xiao
author_facet	Beiwen Zheng Hao Xu Chen Huang Xiao Yu Lihua Guo Huiming Han Jing Zhang Xiawei Jiang Chunlei Chen Yonghong Xiao
author_sort	Beiwen Zheng
title	Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer
title_short	Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer
title_full	Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer
title_fullStr	Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer
title_full_unstemmed	Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer
title_sort	occurrence and genomic characterization of two mcr-1-producing <named-content content-type="genus-species">escherichia coli</named-content> isolates from the same mink farmer
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/61b8ad024db34833a5fadf1591058357
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Occurrence and Genomic Characterization of Two MCR-1-Producing <named-content content-type="genus-species">Escherichia coli</named-content> Isolates from the Same Mink Farmer

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