Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method

Dexamethasone sodium phosphate (DSP) is an ester of dexamethasone with anti-inflammatory action. This study provides new insights to develop a simple, precise, and accurate spectrophotometric method for the quantitative determination of DSP in bulk and pharmaceuticals. The method was validated befor...

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Autores principales:	Mohammed Fanokh Al-Owaidi, Sura L Alkhafaji, Abdulbari Mahdi Mahood
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2021
Materias:	dexamethasone sodium phosphate phosphate buffer ultraviolet spectrophotometry validation Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/61be89fed4fc42e4b8e0ed33f989da72
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spelling	oai:doaj.org-article:61be89fed4fc42e4b8e0ed33f989da722021-11-12T10:17:53ZQuantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method2231-40400976-209410.4103/japtr.japtr_6_21https://doaj.org/article/61be89fed4fc42e4b8e0ed33f989da722021-01-01T00:00:00Zhttp://www.japtr.org/article.asp?issn=2231-4040;year=2021;volume=12;issue=4;spage=378;epage=383;aulast=Al-Owaidihttps://doaj.org/toc/2231-4040https://doaj.org/toc/0976-2094Dexamethasone sodium phosphate (DSP) is an ester of dexamethasone with anti-inflammatory action. This study provides new insights to develop a simple, precise, and accurate spectrophotometric method for the quantitative determination of DSP in bulk and pharmaceuticals. The method was validated before being applied to determine the DSP in six pharmaceutical injection forms from different companies. DSP is soluble in phosphate buffer, so it was used as a solvent, and a pH of 6 was found to be suitable for determination purposes. The DSP solution was scanned in the ultraviolet range (200–400 nm) using a double-beam spectrophotometer with a 1-cm quartz cell. The wavelength (λ max) of DSP was set at 242.5 nm, following the Beer–Lambert law for concentrations from 2 to 50 μg/ml. Dexa AIWA (Germany) showed the best results, being very close to the bulk value with no significant variation. Similarly, Dexamed (Cyprus) and HEMAZON (Syria) showed no significant differences from the bulk; however, the three remaining injections, DEXAKAL (India), DEXABRU (India), and DEXARON (China), showed significant variations from the bulk. Estimated limit of detection and limit of quantitation values for DSP were 0.83 and 2.5 μg/ml, respectively, with a regression coefficient of 0.999. Recovery studies were then used to determine the accuracy of the suggested method. The percentage of recovery was found to be 98.58%–102.52%. All results are suggesting a pivotal method for the routine analysis of DSP both in pure form and the commercially pharmaceutical forms.Mohammed Fanokh Al-OwaidiSura L AlkhafajiAbdulbari Mahdi MahoodWolters Kluwer Medknow Publicationsarticledexamethasone sodium phosphatephosphate bufferultraviolet spectrophotometryvalidationTherapeutics. PharmacologyRM1-950Pharmacy and materia medicaRS1-441ENJournal of Advanced Pharmaceutical Technology & Research, Vol 12, Iss 4, Pp 378-383 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	dexamethasone sodium phosphate phosphate buffer ultraviolet spectrophotometry validation Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441
spellingShingle	dexamethasone sodium phosphate phosphate buffer ultraviolet spectrophotometry validation Therapeutics. Pharmacology RM1-950 Pharmacy and materia medica RS1-441 Mohammed Fanokh Al-Owaidi Sura L Alkhafaji Abdulbari Mahdi Mahood Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method
description	Dexamethasone sodium phosphate (DSP) is an ester of dexamethasone with anti-inflammatory action. This study provides new insights to develop a simple, precise, and accurate spectrophotometric method for the quantitative determination of DSP in bulk and pharmaceuticals. The method was validated before being applied to determine the DSP in six pharmaceutical injection forms from different companies. DSP is soluble in phosphate buffer, so it was used as a solvent, and a pH of 6 was found to be suitable for determination purposes. The DSP solution was scanned in the ultraviolet range (200–400 nm) using a double-beam spectrophotometer with a 1-cm quartz cell. The wavelength (λ max) of DSP was set at 242.5 nm, following the Beer–Lambert law for concentrations from 2 to 50 μg/ml. Dexa AIWA (Germany) showed the best results, being very close to the bulk value with no significant variation. Similarly, Dexamed (Cyprus) and HEMAZON (Syria) showed no significant differences from the bulk; however, the three remaining injections, DEXAKAL (India), DEXABRU (India), and DEXARON (China), showed significant variations from the bulk. Estimated limit of detection and limit of quantitation values for DSP were 0.83 and 2.5 μg/ml, respectively, with a regression coefficient of 0.999. Recovery studies were then used to determine the accuracy of the suggested method. The percentage of recovery was found to be 98.58%–102.52%. All results are suggesting a pivotal method for the routine analysis of DSP both in pure form and the commercially pharmaceutical forms.
format	article
author	Mohammed Fanokh Al-Owaidi Sura L Alkhafaji Abdulbari Mahdi Mahood
author_facet	Mohammed Fanokh Al-Owaidi Sura L Alkhafaji Abdulbari Mahdi Mahood
author_sort	Mohammed Fanokh Al-Owaidi
title	Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method
title_short	Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method
title_full	Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method
title_fullStr	Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method
title_full_unstemmed	Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method
title_sort	quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable ph values using the spectrophotometric method
publisher	Wolters Kluwer Medknow Publications
publishDate	2021
url	https://doaj.org/article/61be89fed4fc42e4b8e0ed33f989da72
work_keys_str_mv	AT mohammedfanokhalowaidi quantitativedeterminationofdexamethasonesodiumphosphateinbulkandpharmaceuticalsatsuitablephvaluesusingthespectrophotometricmethod AT suralalkhafaji quantitativedeterminationofdexamethasonesodiumphosphateinbulkandpharmaceuticalsatsuitablephvaluesusingthespectrophotometricmethod AT abdulbarimahdimahood quantitativedeterminationofdexamethasonesodiumphosphateinbulkandpharmaceuticalsatsuitablephvaluesusingthespectrophotometricmethod
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Quantitative determination of dexamethasone sodium phosphate in bulk and pharmaceuticals at suitable pH values using the spectrophotometric method

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