Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide

Abstract Shiraia bambusicola has been used as a traditional Chinese medicine for a long history. Its major medicinal active metabolites are perylenequinones, including hypocrellin A, elsinochrome A and so on. At present, the fermentation yield of perylenequinones is low, and its complex biosynthesis...

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Autores principales:	Ning Zhao, Yingying Yu, Yunxia Yue, Mingzhu Dou, Bingjing Guo, Shuzhen Yan, Shuanglin Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61c0b99e7d9b40879b83322c152c449c
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spelling	oai:doaj.org-article:61c0b99e7d9b40879b83322c152c449c2021-12-02T14:16:07ZNitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide10.1038/s41598-021-81990-22045-2322https://doaj.org/article/61c0b99e7d9b40879b83322c152c449c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81990-2https://doaj.org/toc/2045-2322Abstract Shiraia bambusicola has been used as a traditional Chinese medicine for a long history. Its major medicinal active metabolites are perylenequinones, including hypocrellin A, elsinochrome A and so on. At present, the fermentation yield of perylenequinones is low, and its complex biosynthesis and regulatory pathways are still unclear. In this study, nitric oxide, as a downstream signal molecule of hydrogen peroxide, regulates the biosynthesis of perylenequinones. Exogenous addition of 0.01 mM sodium nitroprusside (nitric oxide donor) can promote perylenequinones production by 156% compared with the control. Further research found that hydrogen peroxide and nitric oxide increased the transcriptional level of the biosynthetic genes of hypocrellin A. The results showed that nitric oxide is involved in the biosynthesis and regulation of perylenequinones in Shiraia bambusicola as a signal molecule. In the future, the yield of perylenequinones can be increased by adding exogenous nitric oxide in fermentation.Ning ZhaoYingying YuYunxia YueMingzhu DouBingjing GuoShuzhen YanShuanglin ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ning Zhao Yingying Yu Yunxia Yue Mingzhu Dou Bingjing Guo Shuzhen Yan Shuanglin Chen Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide
description	Abstract Shiraia bambusicola has been used as a traditional Chinese medicine for a long history. Its major medicinal active metabolites are perylenequinones, including hypocrellin A, elsinochrome A and so on. At present, the fermentation yield of perylenequinones is low, and its complex biosynthesis and regulatory pathways are still unclear. In this study, nitric oxide, as a downstream signal molecule of hydrogen peroxide, regulates the biosynthesis of perylenequinones. Exogenous addition of 0.01 mM sodium nitroprusside (nitric oxide donor) can promote perylenequinones production by 156% compared with the control. Further research found that hydrogen peroxide and nitric oxide increased the transcriptional level of the biosynthetic genes of hypocrellin A. The results showed that nitric oxide is involved in the biosynthesis and regulation of perylenequinones in Shiraia bambusicola as a signal molecule. In the future, the yield of perylenequinones can be increased by adding exogenous nitric oxide in fermentation.
format	article
author	Ning Zhao Yingying Yu Yunxia Yue Mingzhu Dou Bingjing Guo Shuzhen Yan Shuanglin Chen
author_facet	Ning Zhao Yingying Yu Yunxia Yue Mingzhu Dou Bingjing Guo Shuzhen Yan Shuanglin Chen
author_sort	Ning Zhao
title	Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide
title_short	Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide
title_full	Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide
title_fullStr	Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide
title_full_unstemmed	Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide
title_sort	nitric oxide regulates perylenequinones biosynthesis in shiraia bambusicola s4201 induced by hydrogen peroxide
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/61c0b99e7d9b40879b83322c152c449c
work_keys_str_mv	AT ningzhao nitricoxideregulatesperylenequinonesbiosynthesisinshiraiabambusicolas4201inducedbyhydrogenperoxide AT yingyingyu nitricoxideregulatesperylenequinonesbiosynthesisinshiraiabambusicolas4201inducedbyhydrogenperoxide AT yunxiayue nitricoxideregulatesperylenequinonesbiosynthesisinshiraiabambusicolas4201inducedbyhydrogenperoxide AT mingzhudou nitricoxideregulatesperylenequinonesbiosynthesisinshiraiabambusicolas4201inducedbyhydrogenperoxide AT bingjingguo nitricoxideregulatesperylenequinonesbiosynthesisinshiraiabambusicolas4201inducedbyhydrogenperoxide AT shuzhenyan nitricoxideregulatesperylenequinonesbiosynthesisinshiraiabambusicolas4201inducedbyhydrogenperoxide AT shuanglinchen nitricoxideregulatesperylenequinonesbiosynthesisinshiraiabambusicolas4201inducedbyhydrogenperoxide
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Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide

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