Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata

Abstract Inflammation is a key factor in the development and complications of various diseases because it has a complex pathogenesis. Andrographis paniculate (Burm. f.) Nees (Chuan Xinlian) is a well-known form of Traditional Chinese Medicine (TCM) applied in clearing heat and detoxification. Also,...

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Autores principales:	Naiqiang Zhu, Jingyi Hou, Ning Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61c59973d6634e4086c8c144ba878828
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spelling	oai:doaj.org-article:61c59973d6634e4086c8c144ba8788282021-12-02T15:37:58ZNetwork pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata10.1038/s41598-021-89257-62045-2322https://doaj.org/article/61c59973d6634e4086c8c144ba8788282021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89257-6https://doaj.org/toc/2045-2322Abstract Inflammation is a key factor in the development and complications of various diseases because it has a complex pathogenesis. Andrographis paniculate (Burm. f.) Nees (Chuan Xinlian) is a well-known form of Traditional Chinese Medicine (TCM) applied in clearing heat and detoxification. Also, it is rich in bioactive lactones, with various anti-inflammatory activities. Here, network pharmacology combined with molecular biology experimental approach was used to predict and verify the potential molecular mechanism of Chuan Xinlian in treating inflammation. The bioactive ingredients of Chuan Xinlian were obtained from the TCMSP database and literature. Besides, the targets of Chuan Xinlian and inflammation were collected based on the multi-source databases and used to generate the PPI network. Network topology analysis and functional enrichment analysis were used to screen hub genes and their mechanisms. Molecular docking simulation was performed to evaluate the binding activity between the predicted hub genes and the bioactive ingredients. Additionally, LPS-induced NO production in RAW264.7 cell inflammatory response, RT-PCR and Western blot were used to validate the efficacy of the Chuan Xinlian in the treatment of inflammation. Network analysis outcomes indicated that five targets (IL-6, VEGFA, PTGST2, TNF-α, and MMP-9) were identified as the key targets of Chuan Xinlian in the treatment of inflammation. Further, molecular docking findings revealed that the majority of the bioactive ingredients exhibited a strong binding efficacy towards the predicted hub genes. Functional analysis results showed that the potential mechanisms were primarily concentrated in key pathways including cancer, immunology, and inflammation process. Moreover, RT-PCR and Western blot analysis indicated that Chuan Xinlian extract suppressed the production of inflammatory mediators with anti-inflammatory effects. Our study shows that Chuan Xinlian potentially exerts an anti-inflammatory effect via key pathways including cancer, immunology, and inflammation process. This suggests that Chuan Xinlian has a potential anti-inflammatory action, thereby providing a scientific reference for clinical studies.Naiqiang ZhuJingyi HouNing YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Naiqiang Zhu Jingyi Hou Ning Yang Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata
description	Abstract Inflammation is a key factor in the development and complications of various diseases because it has a complex pathogenesis. Andrographis paniculate (Burm. f.) Nees (Chuan Xinlian) is a well-known form of Traditional Chinese Medicine (TCM) applied in clearing heat and detoxification. Also, it is rich in bioactive lactones, with various anti-inflammatory activities. Here, network pharmacology combined with molecular biology experimental approach was used to predict and verify the potential molecular mechanism of Chuan Xinlian in treating inflammation. The bioactive ingredients of Chuan Xinlian were obtained from the TCMSP database and literature. Besides, the targets of Chuan Xinlian and inflammation were collected based on the multi-source databases and used to generate the PPI network. Network topology analysis and functional enrichment analysis were used to screen hub genes and their mechanisms. Molecular docking simulation was performed to evaluate the binding activity between the predicted hub genes and the bioactive ingredients. Additionally, LPS-induced NO production in RAW264.7 cell inflammatory response, RT-PCR and Western blot were used to validate the efficacy of the Chuan Xinlian in the treatment of inflammation. Network analysis outcomes indicated that five targets (IL-6, VEGFA, PTGST2, TNF-α, and MMP-9) were identified as the key targets of Chuan Xinlian in the treatment of inflammation. Further, molecular docking findings revealed that the majority of the bioactive ingredients exhibited a strong binding efficacy towards the predicted hub genes. Functional analysis results showed that the potential mechanisms were primarily concentrated in key pathways including cancer, immunology, and inflammation process. Moreover, RT-PCR and Western blot analysis indicated that Chuan Xinlian extract suppressed the production of inflammatory mediators with anti-inflammatory effects. Our study shows that Chuan Xinlian potentially exerts an anti-inflammatory effect via key pathways including cancer, immunology, and inflammation process. This suggests that Chuan Xinlian has a potential anti-inflammatory action, thereby providing a scientific reference for clinical studies.
format	article
author	Naiqiang Zhu Jingyi Hou Ning Yang
author_facet	Naiqiang Zhu Jingyi Hou Ning Yang
author_sort	Naiqiang Zhu
title	Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata
title_short	Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata
title_full	Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata
title_fullStr	Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata
title_full_unstemmed	Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata
title_sort	network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of andrographis paniculata
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/61c59973d6634e4086c8c144ba878828
work_keys_str_mv	AT naiqiangzhu networkpharmacologyintegratedwithexperimentalvalidationrevealedtheantiinflammatoryeffectsofandrographispaniculata AT jingyihou networkpharmacologyintegratedwithexperimentalvalidationrevealedtheantiinflammatoryeffectsofandrographispaniculata AT ningyang networkpharmacologyintegratedwithexperimentalvalidationrevealedtheantiinflammatoryeffectsofandrographispaniculata
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Network pharmacology integrated with experimental validation revealed the anti-inflammatory effects of Andrographis paniculata

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