Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.

In a typing system based on opsonic antibodies against carbohydrate antigens of the cell envelope, 60% of Enterococcus faecalis strains can be assigned to one of four serotypes (CPS-A to CPS-D). The structural basis for enterococcal serotypes, however, is still incompletely understood. Here we demon...

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Autores principales:	Christian Theilacker, Zbigniew Kaczyński, Andrea Kropec, Irina Sava, Libin Ye, Anna Bychowska, Otto Holst, Johannes Huebner
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61d2a911b2eb45cbb67f6d9eee4232db
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spelling	oai:doaj.org-article:61d2a911b2eb45cbb67f6d9eee4232db2021-11-18T06:57:09ZSerodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.1932-620310.1371/journal.pone.0017839https://doaj.org/article/61d2a911b2eb45cbb67f6d9eee4232db2011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21437253/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In a typing system based on opsonic antibodies against carbohydrate antigens of the cell envelope, 60% of Enterococcus faecalis strains can be assigned to one of four serotypes (CPS-A to CPS-D). The structural basis for enterococcal serotypes, however, is still incompletely understood. Here we demonstrate that antibodies raised against lipoteichoic acid (LTA) from a CPS-A strain are opsonic to both CPS-A and CPS-B strains. LTA-specific antibodies also bind to LTA of CPS-C and CPS-D strains, but fail to opsonize them. From CPS-C and CPS-D strains resistant to opsonization by anti-LTA, we purified a novel diheteroglycan with a repeating unit of →6)-β-Galf-(1→3)- β-D-Glcp-(1→ with O-acetylation in position 5 and lactic acid substitution at position 3 of the Galf residue. The purified diheteroglycan, but not LTA absorbed opsonic antibodies from whole cell antiserum against E. faecalis type 2 (a CPS-C strain) and type 5 (CPS-D). Rabbit antiserum raised against purified diheteroglycan opsonized CPS-C and CPS-D strains and passive protection with diheteroglycan-specific antiserum reduced bacterial counts by 1.4-3.4 logs in mice infected with E. faecalis strains of the CPS-C and CPS-D serotype. Diheteroglycan-specific opsonic antibodies were absorbed by whole bacterial cells of E. faecalis FA2-2 (CPS-C) but not by its isogenic acapsular cpsI-mutant and on native PAGE purified diheteroglycan co-migrated with the gene product of the cps-locus, suggesting that it is synthesized by this locus. In summary, two polysaccharide antigens, LTA and a novel diheteroglycan, are targets of opsonic antibodies against typeable E. faecalis strains. These cell-wall associated polymers are promising candidates for active and passive vaccination and add to our armamentarium to fight this important nosocomial pathogen.Christian TheilackerZbigniew KaczyńskiAndrea KropecIrina SavaLibin YeAnna BychowskaOtto HolstJohannes HuebnerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17839 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Theilacker Zbigniew Kaczyński Andrea Kropec Irina Sava Libin Ye Anna Bychowska Otto Holst Johannes Huebner Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.
description	In a typing system based on opsonic antibodies against carbohydrate antigens of the cell envelope, 60% of Enterococcus faecalis strains can be assigned to one of four serotypes (CPS-A to CPS-D). The structural basis for enterococcal serotypes, however, is still incompletely understood. Here we demonstrate that antibodies raised against lipoteichoic acid (LTA) from a CPS-A strain are opsonic to both CPS-A and CPS-B strains. LTA-specific antibodies also bind to LTA of CPS-C and CPS-D strains, but fail to opsonize them. From CPS-C and CPS-D strains resistant to opsonization by anti-LTA, we purified a novel diheteroglycan with a repeating unit of →6)-β-Galf-(1→3)- β-D-Glcp-(1→ with O-acetylation in position 5 and lactic acid substitution at position 3 of the Galf residue. The purified diheteroglycan, but not LTA absorbed opsonic antibodies from whole cell antiserum against E. faecalis type 2 (a CPS-C strain) and type 5 (CPS-D). Rabbit antiserum raised against purified diheteroglycan opsonized CPS-C and CPS-D strains and passive protection with diheteroglycan-specific antiserum reduced bacterial counts by 1.4-3.4 logs in mice infected with E. faecalis strains of the CPS-C and CPS-D serotype. Diheteroglycan-specific opsonic antibodies were absorbed by whole bacterial cells of E. faecalis FA2-2 (CPS-C) but not by its isogenic acapsular cpsI-mutant and on native PAGE purified diheteroglycan co-migrated with the gene product of the cps-locus, suggesting that it is synthesized by this locus. In summary, two polysaccharide antigens, LTA and a novel diheteroglycan, are targets of opsonic antibodies against typeable E. faecalis strains. These cell-wall associated polymers are promising candidates for active and passive vaccination and add to our armamentarium to fight this important nosocomial pathogen.
format	article
author	Christian Theilacker Zbigniew Kaczyński Andrea Kropec Irina Sava Libin Ye Anna Bychowska Otto Holst Johannes Huebner
author_facet	Christian Theilacker Zbigniew Kaczyński Andrea Kropec Irina Sava Libin Ye Anna Bychowska Otto Holst Johannes Huebner
author_sort	Christian Theilacker
title	Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.
title_short	Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.
title_full	Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.
title_fullStr	Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.
title_full_unstemmed	Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.
title_sort	serodiversity of opsonic antibodies against enterococcus faecalis--glycans of the cell wall revisited.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/61d2a911b2eb45cbb67f6d9eee4232db
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Serodiversity of opsonic antibodies against Enterococcus faecalis--glycans of the cell wall revisited.

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