The application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.

In December 2012, an outbreak of acute gastrointestinal illness occurred in a geographical distinct area in Denmark covering 368 households. A combined microbiological, epidemiological and environmental investigation was initiated to understand the outbreak magnitude, pathogen(s) and vehicle in orde...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Lieke B van Alphen, Frédérique Dorléans, Anna Charlotte Schultz, Jannik Fonager, Steen Ethelberg, Camilla Dalgaard, Marianne Adelhardt, Jørgen H Engberg, Thea Kølsen Fischer, Sofie Gillesberg Lassen
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/61da78bbc686453ea3fdd8130c4001e4
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:61da78bbc686453ea3fdd8130c4001e4
record_format dspace
spelling oai:doaj.org-article:61da78bbc686453ea3fdd8130c4001e42021-11-25T06:00:39ZThe application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.1932-620310.1371/journal.pone.0105053https://doaj.org/article/61da78bbc686453ea3fdd8130c4001e42014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0105053https://doaj.org/toc/1932-6203In December 2012, an outbreak of acute gastrointestinal illness occurred in a geographical distinct area in Denmark covering 368 households. A combined microbiological, epidemiological and environmental investigation was initiated to understand the outbreak magnitude, pathogen(s) and vehicle in order to control the outbreak. Norovirus GII.4 New Orleans 2009 variant was detected in 15 of 17 individual stool samples from 14 households. Norovirus genomic material from water samples was detected and quantified and sequencing of longer parts of the viral capsid region (>1000 nt) were applied to patient and water samples. All five purposely selected water samples tested positive for norovirus GII in levels up to 1.8×10(4) genomic units per 200 ml. Identical norovirus sequences were found in all 5 sequenced stool samples and 1 sequenced water sample, a second sequenced water sample showed 1 nt (<0.1%) difference. In a cohort study, including 256 participants, cases were defined as residents of the area experiencing diarrhoea or vomiting onset on 12-14 December 2012. We found an attack rate of 51%. Being a case was associated with drinking tap-water on 12-13 December (relative risk = 6.0, 95%CI: 1.6-22) and a dose-response relation for the mean glasses of tap-water consumed was observed. Environmental investigations suggested contamination from a sewage pipe to the drinking water due to fall in pressure during water supply system renovations. The combined microbiological, epidemiological and environmental investigations strongly indicates the outbreak was caused by norovirus contamination of the water supply system.Lieke B van AlphenFrédérique DorléansAnna Charlotte SchultzJannik FonagerSteen EthelbergCamilla DalgaardMarianne AdelhardtJørgen H EngbergThea Kølsen FischerSofie Gillesberg LassenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e105053 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lieke B van Alphen
Frédérique Dorléans
Anna Charlotte Schultz
Jannik Fonager
Steen Ethelberg
Camilla Dalgaard
Marianne Adelhardt
Jørgen H Engberg
Thea Kølsen Fischer
Sofie Gillesberg Lassen
The application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.
description In December 2012, an outbreak of acute gastrointestinal illness occurred in a geographical distinct area in Denmark covering 368 households. A combined microbiological, epidemiological and environmental investigation was initiated to understand the outbreak magnitude, pathogen(s) and vehicle in order to control the outbreak. Norovirus GII.4 New Orleans 2009 variant was detected in 15 of 17 individual stool samples from 14 households. Norovirus genomic material from water samples was detected and quantified and sequencing of longer parts of the viral capsid region (>1000 nt) were applied to patient and water samples. All five purposely selected water samples tested positive for norovirus GII in levels up to 1.8×10(4) genomic units per 200 ml. Identical norovirus sequences were found in all 5 sequenced stool samples and 1 sequenced water sample, a second sequenced water sample showed 1 nt (<0.1%) difference. In a cohort study, including 256 participants, cases were defined as residents of the area experiencing diarrhoea or vomiting onset on 12-14 December 2012. We found an attack rate of 51%. Being a case was associated with drinking tap-water on 12-13 December (relative risk = 6.0, 95%CI: 1.6-22) and a dose-response relation for the mean glasses of tap-water consumed was observed. Environmental investigations suggested contamination from a sewage pipe to the drinking water due to fall in pressure during water supply system renovations. The combined microbiological, epidemiological and environmental investigations strongly indicates the outbreak was caused by norovirus contamination of the water supply system.
format article
author Lieke B van Alphen
Frédérique Dorléans
Anna Charlotte Schultz
Jannik Fonager
Steen Ethelberg
Camilla Dalgaard
Marianne Adelhardt
Jørgen H Engberg
Thea Kølsen Fischer
Sofie Gillesberg Lassen
author_facet Lieke B van Alphen
Frédérique Dorléans
Anna Charlotte Schultz
Jannik Fonager
Steen Ethelberg
Camilla Dalgaard
Marianne Adelhardt
Jørgen H Engberg
Thea Kølsen Fischer
Sofie Gillesberg Lassen
author_sort Lieke B van Alphen
title The application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.
title_short The application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.
title_full The application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.
title_fullStr The application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.
title_full_unstemmed The application of new molecular methods in the investigation of a waterborne outbreak of norovirus in Denmark, 2012.
title_sort application of new molecular methods in the investigation of a waterborne outbreak of norovirus in denmark, 2012.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/61da78bbc686453ea3fdd8130c4001e4
work_keys_str_mv AT liekebvanalphen theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT frederiquedorleans theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT annacharlotteschultz theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT jannikfonager theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT steenethelberg theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT camilladalgaard theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT marianneadelhardt theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT jørgenhengberg theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT theakølsenfischer theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT sofiegillesberglassen theapplicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT liekebvanalphen applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT frederiquedorleans applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT annacharlotteschultz applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT jannikfonager applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT steenethelberg applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT camilladalgaard applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT marianneadelhardt applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT jørgenhengberg applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT theakølsenfischer applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
AT sofiegillesberglassen applicationofnewmolecularmethodsintheinvestigationofawaterborneoutbreakofnorovirusindenmark2012
_version_ 1718414285901660160