The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells

The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells

Lysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ di...

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Autores principales: Weihua Tian, Zilu Ye, Shengjun Wang, Morten Alder Schulz, Julie Van Coillie, Lingbo Sun, Yen-Hsi Chen, Yoshiki Narimatsu, Lars Hansen, Claus Kristensen, Ulla Mandel, Eric Paul Bennett, Siamak Jabbarzadeh-Tabrizi, Raphael Schiffmann, Jin-Song Shen, Sergey Y. Vakhrushev, Henrik Clausen, Zhang Yang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/61e178a4b78e40d9866c7f7e1c00ad4a
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spelling oai:doaj.org-article:61e178a4b78e40d9866c7f7e1c00ad4a2021-12-02T17:02:16ZThe glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells10.1038/s41467-019-09809-32041-1723https://doaj.org/article/61e178a4b78e40d9866c7f7e1c00ad4a2019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09809-3https://doaj.org/toc/2041-1723Lysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ distribution.Weihua TianZilu YeShengjun WangMorten Alder SchulzJulie Van CoillieLingbo SunYen-Hsi ChenYoshiki NarimatsuLars HansenClaus KristensenUlla MandelEric Paul BennettSiamak Jabbarzadeh-TabriziRaphael SchiffmannJin-Song ShenSergey Y. VakhrushevHenrik ClausenZhang YangNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Weihua Tian
Zilu Ye
Shengjun Wang
Morten Alder Schulz
Julie Van Coillie
Lingbo Sun
Yen-Hsi Chen
Yoshiki Narimatsu
Lars Hansen
Claus Kristensen
Ulla Mandel
Eric Paul Bennett
Siamak Jabbarzadeh-Tabrizi
Raphael Schiffmann
Jin-Song Shen
Sergey Y. Vakhrushev
Henrik Clausen
Zhang Yang
The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
description Lysosomal replacement enzymes are taken up by cell surface receptors that recognize glycans, the effects of different glycan features are unknown. Here the authors present a gene engineering screen in CHO cells that allows custom N-glycan-decorated enzymes with improved circulation time and organ distribution.
format article
author Weihua Tian
Zilu Ye
Shengjun Wang
Morten Alder Schulz
Julie Van Coillie
Lingbo Sun
Yen-Hsi Chen
Yoshiki Narimatsu
Lars Hansen
Claus Kristensen
Ulla Mandel
Eric Paul Bennett
Siamak Jabbarzadeh-Tabrizi
Raphael Schiffmann
Jin-Song Shen
Sergey Y. Vakhrushev
Henrik Clausen
Zhang Yang
author_facet Weihua Tian
Zilu Ye
Shengjun Wang
Morten Alder Schulz
Julie Van Coillie
Lingbo Sun
Yen-Hsi Chen
Yoshiki Narimatsu
Lars Hansen
Claus Kristensen
Ulla Mandel
Eric Paul Bennett
Siamak Jabbarzadeh-Tabrizi
Raphael Schiffmann
Jin-Song Shen
Sergey Y. Vakhrushev
Henrik Clausen
Zhang Yang
author_sort Weihua Tian
title The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
title_short The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
title_full The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
title_fullStr The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
title_full_unstemmed The glycosylation design space for recombinant lysosomal replacement enzymes produced in CHO cells
title_sort glycosylation design space for recombinant lysosomal replacement enzymes produced in cho cells
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/61e178a4b78e40d9866c7f7e1c00ad4a
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