Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.

<h4>Background</h4>Among Coagulase-Negative Staphylococci (CoNS), Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCm...

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Autores principales:	Soraya Mendoza-Olazarán, Rayo Morfin-Otero, Eduardo Rodríguez-Noriega, Jorge Llaca-Díaz, Samantha Flores-Treviño, Gloria Ma González-González, Licet Villarreal-Treviño, Elvira Garza-González
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61e6e1c1569f4c478e6e677c68df38f9
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spelling	oai:doaj.org-article:61e6e1c1569f4c478e6e677c68df38f92021-11-18T07:50:03ZMicrobiological and molecular characterization of Staphylococcus hominis isolates from blood.1932-620310.1371/journal.pone.0061161https://doaj.org/article/61e6e1c1569f4c478e6e677c68df38f92013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23585877/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Among Coagulase-Negative Staphylococci (CoNS), Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCmec (Staphylococcal Chromosomal Cassette mec) type, and genetic relatedness of clinical S. hominis isolates.<h4>Methodology</h4>S. hominis blood isolates (n = 21) were screened for biofilm formation using crystal violet staining. Methicillin resistance was evaluated using the cefoxitin disk test and the mecA gene was detected by PCR. Antibiotic resistance was determined by the broth microdilution method. Genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE) and SCCmec typed by multiplex PCR using two different methodologies described for Staphylococcus aureus.<h4>Results</h4>Of the S. hominis isolates screened, 47.6% (10/21) were categorized as strong biofilm producers and 23.8% (5/21) as weak producers. Furthermore, 81% (17/21) of the isolates were methicillin resistant and mecA gene carriers. Resistance to ampicillin, erythromycin, and trimethoprim was observed in >70% of isolates screened. Each isolate showed a different PFGE macrorestriction pattern with similarity ranging between 0-95%. Among mecA-positive isolates, 14 (82%) harbored a non-typeable SCCmec type: eight isolates were not positive for any ccr complex; four contained the mec complex A ccrAB1 and ccrC, one isolate contained mec complex A, ccrAB4 and ccrC, and one isolate contained the mec complex A, ccrAB1, ccrAB4, and ccrC. Two isolates harbored the association: mec complex A and ccrAB1. Only one strain was typeable as SCCmec III.<h4>Conclusions</h4>The S. hominis isolates analyzed were variable biofilm producers had a high prevalence of methicillin resistance and resistance to other antibiotics, and high genetic diversity. The results of this study strongly suggested that S. hominis isolates harbor new SCCmec structural elements and might be reservoirs of ccrC1 in addition to ccrAB1 and mec complex A.Soraya Mendoza-OlazaránRayo Morfin-OteroEduardo Rodríguez-NoriegaJorge Llaca-DíazSamantha Flores-TreviñoGloria Ma González-GonzálezLicet Villarreal-TreviñoElvira Garza-GonzálezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61161 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Soraya Mendoza-Olazarán Rayo Morfin-Otero Eduardo Rodríguez-Noriega Jorge Llaca-Díaz Samantha Flores-Treviño Gloria Ma González-González Licet Villarreal-Treviño Elvira Garza-González Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.
description	<h4>Background</h4>Among Coagulase-Negative Staphylococci (CoNS), Staphylococcus hominis represents the third most common organism recoverable from the blood of immunocompromised patients. The aim of this study was to characterize biofilm formation, antibiotic resistance, define the SCCmec (Staphylococcal Chromosomal Cassette mec) type, and genetic relatedness of clinical S. hominis isolates.<h4>Methodology</h4>S. hominis blood isolates (n = 21) were screened for biofilm formation using crystal violet staining. Methicillin resistance was evaluated using the cefoxitin disk test and the mecA gene was detected by PCR. Antibiotic resistance was determined by the broth microdilution method. Genetic relatedness was determined by pulsed-field gel electrophoresis (PFGE) and SCCmec typed by multiplex PCR using two different methodologies described for Staphylococcus aureus.<h4>Results</h4>Of the S. hominis isolates screened, 47.6% (10/21) were categorized as strong biofilm producers and 23.8% (5/21) as weak producers. Furthermore, 81% (17/21) of the isolates were methicillin resistant and mecA gene carriers. Resistance to ampicillin, erythromycin, and trimethoprim was observed in >70% of isolates screened. Each isolate showed a different PFGE macrorestriction pattern with similarity ranging between 0-95%. Among mecA-positive isolates, 14 (82%) harbored a non-typeable SCCmec type: eight isolates were not positive for any ccr complex; four contained the mec complex A ccrAB1 and ccrC, one isolate contained mec complex A, ccrAB4 and ccrC, and one isolate contained the mec complex A, ccrAB1, ccrAB4, and ccrC. Two isolates harbored the association: mec complex A and ccrAB1. Only one strain was typeable as SCCmec III.<h4>Conclusions</h4>The S. hominis isolates analyzed were variable biofilm producers had a high prevalence of methicillin resistance and resistance to other antibiotics, and high genetic diversity. The results of this study strongly suggested that S. hominis isolates harbor new SCCmec structural elements and might be reservoirs of ccrC1 in addition to ccrAB1 and mec complex A.
format	article
author	Soraya Mendoza-Olazarán Rayo Morfin-Otero Eduardo Rodríguez-Noriega Jorge Llaca-Díaz Samantha Flores-Treviño Gloria Ma González-González Licet Villarreal-Treviño Elvira Garza-González
author_facet	Soraya Mendoza-Olazarán Rayo Morfin-Otero Eduardo Rodríguez-Noriega Jorge Llaca-Díaz Samantha Flores-Treviño Gloria Ma González-González Licet Villarreal-Treviño Elvira Garza-González
author_sort	Soraya Mendoza-Olazarán
title	Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.
title_short	Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.
title_full	Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.
title_fullStr	Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.
title_full_unstemmed	Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.
title_sort	microbiological and molecular characterization of staphylococcus hominis isolates from blood.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/61e6e1c1569f4c478e6e677c68df38f9
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Microbiological and molecular characterization of Staphylococcus hominis isolates from blood.

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