Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle

Abstract This study investigates peptide components of L-selectin ligand (LSL) and their gene expressions in human endometrium during the natural menstrual cycle. We recruited 41 endometrial samples from reproductive-aged women with leiomyoma and undergoing hysterectomy and 11 endometrial samples fr...

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Autores principales:	Tsung-Hsuan Lai, Fung-Wei Chang, Jun-Jie Lin, Qing-Dong Ling
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61ea4ff860144cce9600e8841b77c24b
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spelling	oai:doaj.org-article:61ea4ff860144cce9600e8841b77c24b2021-12-02T15:07:44ZGene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle10.1038/s41598-018-19911-z2045-2322https://doaj.org/article/61ea4ff860144cce9600e8841b77c24b2018-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-19911-zhttps://doaj.org/toc/2045-2322Abstract This study investigates peptide components of L-selectin ligand (LSL) and their gene expressions in human endometrium during the natural menstrual cycle. We recruited 41 endometrial samples from reproductive-aged women with leiomyoma and undergoing hysterectomy and 11 endometrial samples from menopausal women as controls. Immunohistochemistry revealed strong MECA-79 expression from the early through the mid-secretory phase and low expression in menopausal endometrium. Five peptide components of LSL were detected in reproductive and menopausal endometrium by one-step quantitative RT-PCR: podocalyxin, endomucin, nepmucin, GlyCAM-1, and CD34. Endomucin differed significantly between the proliferative and early-secretory phases. CHST2 and CHST4 genes (which are involved in the generation of LSL epitopes) were expressed without significant differences among phases. The gene expression of progesterone receptor decreased from the proliferative to the late-secretory phase, and the difference was significant. However, estrogen receptor α expression showed stability among phases. The significant expression of endomucin between the proliferative and early-secretory phases might play a vital role in endometrial receptivity. Further studies are needed to investigate the factors that regulate the expression of endomucin and other LSL peptide components in different phases of the menstrual cycle.Tsung-Hsuan LaiFung-Wei ChangJun-Jie LinQing-Dong LingNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tsung-Hsuan Lai Fung-Wei Chang Jun-Jie Lin Qing-Dong Ling Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle
description	Abstract This study investigates peptide components of L-selectin ligand (LSL) and their gene expressions in human endometrium during the natural menstrual cycle. We recruited 41 endometrial samples from reproductive-aged women with leiomyoma and undergoing hysterectomy and 11 endometrial samples from menopausal women as controls. Immunohistochemistry revealed strong MECA-79 expression from the early through the mid-secretory phase and low expression in menopausal endometrium. Five peptide components of LSL were detected in reproductive and menopausal endometrium by one-step quantitative RT-PCR: podocalyxin, endomucin, nepmucin, GlyCAM-1, and CD34. Endomucin differed significantly between the proliferative and early-secretory phases. CHST2 and CHST4 genes (which are involved in the generation of LSL epitopes) were expressed without significant differences among phases. The gene expression of progesterone receptor decreased from the proliferative to the late-secretory phase, and the difference was significant. However, estrogen receptor α expression showed stability among phases. The significant expression of endomucin between the proliferative and early-secretory phases might play a vital role in endometrial receptivity. Further studies are needed to investigate the factors that regulate the expression of endomucin and other LSL peptide components in different phases of the menstrual cycle.
format	article
author	Tsung-Hsuan Lai Fung-Wei Chang Jun-Jie Lin Qing-Dong Ling
author_facet	Tsung-Hsuan Lai Fung-Wei Chang Jun-Jie Lin Qing-Dong Ling
author_sort	Tsung-Hsuan Lai
title	Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle
title_short	Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle
title_full	Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle
title_fullStr	Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle
title_full_unstemmed	Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle
title_sort	gene expression of human endometrial l-selectin ligand in relation to the phases of the natural menstrual cycle
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/61ea4ff860144cce9600e8841b77c24b
work_keys_str_mv	AT tsunghsuanlai geneexpressionofhumanendometriallselectinligandinrelationtothephasesofthenaturalmenstrualcycle AT fungweichang geneexpressionofhumanendometriallselectinligandinrelationtothephasesofthenaturalmenstrualcycle AT junjielin geneexpressionofhumanendometriallselectinligandinrelationtothephasesofthenaturalmenstrualcycle AT qingdongling geneexpressionofhumanendometriallselectinligandinrelationtothephasesofthenaturalmenstrualcycle
_version_	1718388389321900032

Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle

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