DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients

Objectives: There have been reports of unexplained visual loss following intra-ocular silicone oil (SiO) tamponade in retinal detachment patients, yet the underlying mechanism is unknown. The aim of this study was to investigate the mechanisms behind retinal toxicity following intra-ocular SiO tampo...

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Autores principales:	Yiyang Shu, Min Gao, Yifan Zhou, Haiyun Liu, Xiaodong Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	data-independent acquisition (DIA) quantitative proteomics silicone oil rhegmatogenous retinal detachment (RRD) vitreous Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/61f7acfcab6d402f97d5191b8fc0cce8
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spelling	oai:doaj.org-article:61f7acfcab6d402f97d5191b8fc0cce82021-12-03T05:57:03ZDIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients2296-889X10.3389/fmolb.2021.763002https://doaj.org/article/61f7acfcab6d402f97d5191b8fc0cce82021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmolb.2021.763002/fullhttps://doaj.org/toc/2296-889XObjectives: There have been reports of unexplained visual loss following intra-ocular silicone oil (SiO) tamponade in retinal detachment patients, yet the underlying mechanism is unknown. The aim of this study was to investigate the mechanisms behind retinal toxicity following intra-ocular SiO tamponade in retinal detachment patients.Methods and Results: Vitreous fluid samples were acquired from 27 patients (27 eyes). Twelve eyes for data-independent acquisition (DIA) were divided into four groups: pars plana vitrectomy (PPV) for rhegmatogenous retinal detachment (RD group), SiO removal after successful retinal reattachment (SO group), cataract surgery after successful retinal reattachment with sterilized air tamponade (FA group), and PPV for epiretinal membrane (ERM group). The remaining 15 eyes were used for enzyme-linked immunosorbent assay analysis. DIA was combined with two-dimensional liquid chromatography–tandem mass spectrometry to find expression changes in the proteome of vitreous. Mean number mass spectra, statistically differentially expressed proteins, gene ontology (GO), pathway representations, and protein interactions were analyzed. GO analysis showed that the protein categories of synapse organization, cell adhesion, and regulation of cell migration in the SO group were differentially expressed compared to the control or FA groups (p < 0.05). Through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, lysosome and cell adhesion were found to be significantly enriched in the SO group compared to the FA and control groups (p < 0.05). Cadherin 2, transferrin, and lysosome function may partially contribute to silicone oil-related vision loss.Conclusion: Vision loss-inducing novel molecular signatures and pathways that may be associated with SiO toxicity were identified. Transferrin may be a potential visual outcome biomarker for SiO tamponade.Yiyang ShuYiyang ShuYiyang ShuYiyang ShuYiyang ShuMin GaoMin GaoMin GaoMin GaoMin GaoYifan ZhouHaiyun LiuHaiyun LiuHaiyun LiuHaiyun LiuHaiyun LiuXiaodong SunXiaodong SunXiaodong SunXiaodong SunXiaodong SunFrontiers Media S.A.articledata-independent acquisition (DIA)quantitative proteomicssilicone oilrhegmatogenous retinal detachment (RRD)vitreousBiology (General)QH301-705.5ENFrontiers in Molecular Biosciences, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	data-independent acquisition (DIA) quantitative proteomics silicone oil rhegmatogenous retinal detachment (RRD) vitreous Biology (General) QH301-705.5
spellingShingle	data-independent acquisition (DIA) quantitative proteomics silicone oil rhegmatogenous retinal detachment (RRD) vitreous Biology (General) QH301-705.5 Yiyang Shu Yiyang Shu Yiyang Shu Yiyang Shu Yiyang Shu Min Gao Min Gao Min Gao Min Gao Min Gao Yifan Zhou Haiyun Liu Haiyun Liu Haiyun Liu Haiyun Liu Haiyun Liu Xiaodong Sun Xiaodong Sun Xiaodong Sun Xiaodong Sun Xiaodong Sun DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients
description	Objectives: There have been reports of unexplained visual loss following intra-ocular silicone oil (SiO) tamponade in retinal detachment patients, yet the underlying mechanism is unknown. The aim of this study was to investigate the mechanisms behind retinal toxicity following intra-ocular SiO tamponade in retinal detachment patients.Methods and Results: Vitreous fluid samples were acquired from 27 patients (27 eyes). Twelve eyes for data-independent acquisition (DIA) were divided into four groups: pars plana vitrectomy (PPV) for rhegmatogenous retinal detachment (RD group), SiO removal after successful retinal reattachment (SO group), cataract surgery after successful retinal reattachment with sterilized air tamponade (FA group), and PPV for epiretinal membrane (ERM group). The remaining 15 eyes were used for enzyme-linked immunosorbent assay analysis. DIA was combined with two-dimensional liquid chromatography–tandem mass spectrometry to find expression changes in the proteome of vitreous. Mean number mass spectra, statistically differentially expressed proteins, gene ontology (GO), pathway representations, and protein interactions were analyzed. GO analysis showed that the protein categories of synapse organization, cell adhesion, and regulation of cell migration in the SO group were differentially expressed compared to the control or FA groups (p < 0.05). Through Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, lysosome and cell adhesion were found to be significantly enriched in the SO group compared to the FA and control groups (p < 0.05). Cadherin 2, transferrin, and lysosome function may partially contribute to silicone oil-related vision loss.Conclusion: Vision loss-inducing novel molecular signatures and pathways that may be associated with SiO toxicity were identified. Transferrin may be a potential visual outcome biomarker for SiO tamponade.
format	article
author	Yiyang Shu Yiyang Shu Yiyang Shu Yiyang Shu Yiyang Shu Min Gao Min Gao Min Gao Min Gao Min Gao Yifan Zhou Haiyun Liu Haiyun Liu Haiyun Liu Haiyun Liu Haiyun Liu Xiaodong Sun Xiaodong Sun Xiaodong Sun Xiaodong Sun Xiaodong Sun
author_facet	Yiyang Shu Yiyang Shu Yiyang Shu Yiyang Shu Yiyang Shu Min Gao Min Gao Min Gao Min Gao Min Gao Yifan Zhou Haiyun Liu Haiyun Liu Haiyun Liu Haiyun Liu Haiyun Liu Xiaodong Sun Xiaodong Sun Xiaodong Sun Xiaodong Sun Xiaodong Sun
author_sort	Yiyang Shu
title	DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients
title_short	DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients
title_full	DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients
title_fullStr	DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients
title_full_unstemmed	DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients
title_sort	dia comparative proteomic analysis of retro-oil fluid and vitreous fluid from retinal detachment patients
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/61f7acfcab6d402f97d5191b8fc0cce8
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DIA Comparative Proteomic Analysis of Retro-oil Fluid and Vitreous Fluid From Retinal Detachment Patients

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