Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>

Spermatogenesis is a process of self-renewal and differentiation in spermatogonial stem cells. During this process, germ cells and somatic cells interact intricately to ensure long-term fertility and accurate genome propagation. Spermatogenesis has been intensely investigated in mammals but remains...

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Autores principales:	Xi Wu, Yang Yang, Chaoyue Zhong, Tong Wang, Yanhong Deng, Hengjin Huang, Haoran Lin, Zining Meng, Xiaochun Liu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	single-cell transcriptome testis spermatogenesis protogynous orange-spotted grouper Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/61f8bab61ad24ad0aa22e81ea4956725
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spelling	oai:doaj.org-article:61f8bab61ad24ad0aa22e81ea49567252021-11-25T17:58:16ZSingle-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>10.3390/ijms2222126071422-00671661-6596https://doaj.org/article/61f8bab61ad24ad0aa22e81ea49567252021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12607https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Spermatogenesis is a process of self-renewal and differentiation in spermatogonial stem cells. During this process, germ cells and somatic cells interact intricately to ensure long-term fertility and accurate genome propagation. Spermatogenesis has been intensely investigated in mammals but remains poorly understood with regard to teleosts. Here, we performed single-cell RNA sequencing of ~9500 testicular cells from the male, orange-spotted grouper. In the adult testis, we divided the cells into nine clusters and defined ten cell types, as compared with human testis data, including cell populations with characteristics of male germ cells and somatic cells, each of which expressed specific marker genes. We also identified and profiled the expression patterns of four marker genes (<i>calr</i>, <i>eef1a</i>, <i>s100a1</i>, <i>vasa</i>) in both the ovary and adult testis. Our data provide a blueprint of male germ cells and supporting somatic cells. Moreover, the cell markers are candidates that could be used for further cell identification.Xi WuYang YangChaoyue ZhongTong WangYanhong DengHengjin HuangHaoran LinZining MengXiaochun LiuMDPI AGarticlesingle-cell transcriptometestisspermatogenesisprotogynousorange-spotted grouperBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12607, p 12607 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	single-cell transcriptome testis spermatogenesis protogynous orange-spotted grouper Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	single-cell transcriptome testis spermatogenesis protogynous orange-spotted grouper Biology (General) QH301-705.5 Chemistry QD1-999 Xi Wu Yang Yang Chaoyue Zhong Tong Wang Yanhong Deng Hengjin Huang Haoran Lin Zining Meng Xiaochun Liu Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>
description	Spermatogenesis is a process of self-renewal and differentiation in spermatogonial stem cells. During this process, germ cells and somatic cells interact intricately to ensure long-term fertility and accurate genome propagation. Spermatogenesis has been intensely investigated in mammals but remains poorly understood with regard to teleosts. Here, we performed single-cell RNA sequencing of ~9500 testicular cells from the male, orange-spotted grouper. In the adult testis, we divided the cells into nine clusters and defined ten cell types, as compared with human testis data, including cell populations with characteristics of male germ cells and somatic cells, each of which expressed specific marker genes. We also identified and profiled the expression patterns of four marker genes (<i>calr</i>, <i>eef1a</i>, <i>s100a1</i>, <i>vasa</i>) in both the ovary and adult testis. Our data provide a blueprint of male germ cells and supporting somatic cells. Moreover, the cell markers are candidates that could be used for further cell identification.
format	article
author	Xi Wu Yang Yang Chaoyue Zhong Tong Wang Yanhong Deng Hengjin Huang Haoran Lin Zining Meng Xiaochun Liu
author_facet	Xi Wu Yang Yang Chaoyue Zhong Tong Wang Yanhong Deng Hengjin Huang Haoran Lin Zining Meng Xiaochun Liu
author_sort	Xi Wu
title	Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>
title_short	Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>
title_full	Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>
title_fullStr	Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>
title_full_unstemmed	Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>
title_sort	single-cell atlas of adult testis in protogynous hermaphroditic orange-spotted grouper, <i>epinephelus coioides</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/61f8bab61ad24ad0aa22e81ea4956725
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Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, <i>Epinephelus coioides</i>

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