Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations

Abstract Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conflicting evidence exists as to their impact on neutrophils and whether they trigger damaging inflammation. Neutrophil’s importance in innate defence and regulating immune...

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Autores principales:	Jennifer A. Fraser, Sadie Kemp, Lesley Young, Mark Ross, Morag Prach, Gary R. Hutchison, Eva Malone
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/61fcc56d8c0947fba710c0fa6677bcfa
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spelling	oai:doaj.org-article:61fcc56d8c0947fba710c0fa6677bcfa2021-12-02T16:08:27ZSilver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations10.1038/s41598-018-25854-22045-2322https://doaj.org/article/61fcc56d8c0947fba710c0fa6677bcfa2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25854-2https://doaj.org/toc/2045-2322Abstract Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conflicting evidence exists as to their impact on neutrophils and whether they trigger damaging inflammation. Neutrophil’s importance in innate defence and regulating immune networks mean it’s essential we understand AgNP’s impact on neutrophil function. Human neutrophil viability following AgNP or Ag Bulk treatment was analysed by flow cytometry and AnV/PI staining. Whilst AgNP exposure did not increase the total number of apoptotic neutrophils, the number of late apoptotic neutrophils was increased, suggesting AgNP increase transit through apoptosis. Mature (CD16bright/CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic (CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil preparations. AgNP exposure significantly reduced CD62L staining of CD16bright/CD62Lbright neutrophils, and increased CD16 staining of CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and maturation, respectively. AgNP exposure dramatically increased IL-8, yet not classical pro-inflammatory cytokine release, suggesting AgNP triggers neutrophil activation, without pro-inflammation or damaging, necrotic cell death. For the first time, we show AgNPs differentially affect distinct sub-populations of circulating human neutrophils; activating mature neutrophils with the emergence of CD16bright/CD62Ldim neutrophils. This may stimulate particle clearance without harmful inflammation, challenging previous assumptions that silver nanomaterials induce neutrophil toxicity and damaging inflammatory responses.Jennifer A. FraserSadie KempLesley YoungMark RossMorag PrachGary R. HutchisonEva MaloneNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer A. Fraser Sadie Kemp Lesley Young Mark Ross Morag Prach Gary R. Hutchison Eva Malone Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
description	Abstract Neutrophil surveillance is central to nanoparticle clearance. Silver nanoparticles (AgNP) have numerous uses, however conflicting evidence exists as to their impact on neutrophils and whether they trigger damaging inflammation. Neutrophil’s importance in innate defence and regulating immune networks mean it’s essential we understand AgNP’s impact on neutrophil function. Human neutrophil viability following AgNP or Ag Bulk treatment was analysed by flow cytometry and AnV/PI staining. Whilst AgNP exposure did not increase the total number of apoptotic neutrophils, the number of late apoptotic neutrophils was increased, suggesting AgNP increase transit through apoptosis. Mature (CD16bright/CD62Lbright), immature (CD16dim/CD62Lbright) and apoptotic (CD16dim/CD62Ldim) neutrophil populations were evident within isolated neutrophil preparations. AgNP exposure significantly reduced CD62L staining of CD16bright/CD62Lbright neutrophils, and increased CD16 staining of CD16dim/CD62Lbright populations, suggesting AgNPs trigger neutrophil activation and maturation, respectively. AgNP exposure dramatically increased IL-8, yet not classical pro-inflammatory cytokine release, suggesting AgNP triggers neutrophil activation, without pro-inflammation or damaging, necrotic cell death. For the first time, we show AgNPs differentially affect distinct sub-populations of circulating human neutrophils; activating mature neutrophils with the emergence of CD16bright/CD62Ldim neutrophils. This may stimulate particle clearance without harmful inflammation, challenging previous assumptions that silver nanomaterials induce neutrophil toxicity and damaging inflammatory responses.
format	article
author	Jennifer A. Fraser Sadie Kemp Lesley Young Mark Ross Morag Prach Gary R. Hutchison Eva Malone
author_facet	Jennifer A. Fraser Sadie Kemp Lesley Young Mark Ross Morag Prach Gary R. Hutchison Eva Malone
author_sort	Jennifer A. Fraser
title	Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
title_short	Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
title_full	Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
title_fullStr	Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
title_full_unstemmed	Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
title_sort	silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/61fcc56d8c0947fba710c0fa6677bcfa
work_keys_str_mv	AT jenniferafraser silvernanoparticlespromotetheemergenceofheterogeneichumanneutrophilsubpopulations AT sadiekemp silvernanoparticlespromotetheemergenceofheterogeneichumanneutrophilsubpopulations AT lesleyyoung silvernanoparticlespromotetheemergenceofheterogeneichumanneutrophilsubpopulations AT markross silvernanoparticlespromotetheemergenceofheterogeneichumanneutrophilsubpopulations AT moragprach silvernanoparticlespromotetheemergenceofheterogeneichumanneutrophilsubpopulations AT garyrhutchison silvernanoparticlespromotetheemergenceofheterogeneichumanneutrophilsubpopulations AT evamalone silvernanoparticlespromotetheemergenceofheterogeneichumanneutrophilsubpopulations
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Silver nanoparticles promote the emergence of heterogeneic human neutrophil sub-populations

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