Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.

Fbh1, an F-box helicase related to bacterial UvrD, has been proposed to modulate homologous recombination in fission yeast. We provide several lines of evidence for such modulation. Fbh1, but not the related helicases Srs2 and Rqh1, suppressed the formation of crossover recombinants from single HO-i...

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Autores principales:	Yasuhiro Tsutsui, Yumiko Kurokawa, Kentaro Ito, Md Shahjahan P Siddique, Yumiko Kawano, Fumiaki Yamao, Hiroshi Iwasaki
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/61fe4b497f8b454681e875d1ff5b4c86
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spelling	oai:doaj.org-article:61fe4b497f8b454681e875d1ff5b4c862021-11-25T05:51:39ZMultiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.1553-73901553-740410.1371/journal.pgen.1004542https://doaj.org/article/61fe4b497f8b454681e875d1ff5b4c862014-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25165823/?tool=EBIhttps://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Fbh1, an F-box helicase related to bacterial UvrD, has been proposed to modulate homologous recombination in fission yeast. We provide several lines of evidence for such modulation. Fbh1, but not the related helicases Srs2 and Rqh1, suppressed the formation of crossover recombinants from single HO-induced DNA double-strand breaks. Purified Fbh1 in complex with Skp1 (Fbh1-Skp1 complex) inhibited Rad51-driven DNA strand exchange by disrupting Rad51 nucleoprotein filaments in an ATP-dependent manner; this disruption was alleviated by the Swi5-Sfr1 complex, an auxiliary activator of Rad51. In addition, the reconstituted SCFFbh1 complex, composed of purified Fbh1-Skp1 and Pcu1-Rbx1, displayed ubiquitin-ligase E3 activity toward Rad51. Furthermore, Fbh1 reduced the protein level of Rad51 in stationary phase in an F-box-dependent, but not in a helicase domain-independent manner. These results suggest that Fbh1 negatively regulates Rad51-mediated homologous recombination via its two putative, unrelated activities, namely DNA unwinding/translocation and ubiquitin ligation. In addition to its anti-recombinase activity, we tentatively suggest that Fbh1 might also have a pro-recombination role in vivo, because the Fbh1-Skp1 complex stimulated Rad51-mediated strand exchange in vitro after strand exchange had been initiated.Yasuhiro TsutsuiYumiko KurokawaKentaro ItoMd Shahjahan P SiddiqueYumiko KawanoFumiaki YamaoHiroshi IwasakiPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 10, Iss 8, p e1004542 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Yasuhiro Tsutsui Yumiko Kurokawa Kentaro Ito Md Shahjahan P Siddique Yumiko Kawano Fumiaki Yamao Hiroshi Iwasaki Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
description	Fbh1, an F-box helicase related to bacterial UvrD, has been proposed to modulate homologous recombination in fission yeast. We provide several lines of evidence for such modulation. Fbh1, but not the related helicases Srs2 and Rqh1, suppressed the formation of crossover recombinants from single HO-induced DNA double-strand breaks. Purified Fbh1 in complex with Skp1 (Fbh1-Skp1 complex) inhibited Rad51-driven DNA strand exchange by disrupting Rad51 nucleoprotein filaments in an ATP-dependent manner; this disruption was alleviated by the Swi5-Sfr1 complex, an auxiliary activator of Rad51. In addition, the reconstituted SCFFbh1 complex, composed of purified Fbh1-Skp1 and Pcu1-Rbx1, displayed ubiquitin-ligase E3 activity toward Rad51. Furthermore, Fbh1 reduced the protein level of Rad51 in stationary phase in an F-box-dependent, but not in a helicase domain-independent manner. These results suggest that Fbh1 negatively regulates Rad51-mediated homologous recombination via its two putative, unrelated activities, namely DNA unwinding/translocation and ubiquitin ligation. In addition to its anti-recombinase activity, we tentatively suggest that Fbh1 might also have a pro-recombination role in vivo, because the Fbh1-Skp1 complex stimulated Rad51-mediated strand exchange in vitro after strand exchange had been initiated.
format	article
author	Yasuhiro Tsutsui Yumiko Kurokawa Kentaro Ito Md Shahjahan P Siddique Yumiko Kawano Fumiaki Yamao Hiroshi Iwasaki
author_facet	Yasuhiro Tsutsui Yumiko Kurokawa Kentaro Ito Md Shahjahan P Siddique Yumiko Kawano Fumiaki Yamao Hiroshi Iwasaki
author_sort	Yasuhiro Tsutsui
title	Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
title_short	Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
title_full	Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
title_fullStr	Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
title_full_unstemmed	Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.
title_sort	multiple regulation of rad51-mediated homologous recombination by fission yeast fbh1.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/61fe4b497f8b454681e875d1ff5b4c86
work_keys_str_mv	AT yasuhirotsutsui multipleregulationofrad51mediatedhomologousrecombinationbyfissionyeastfbh1 AT yumikokurokawa multipleregulationofrad51mediatedhomologousrecombinationbyfissionyeastfbh1 AT kentaroito multipleregulationofrad51mediatedhomologousrecombinationbyfissionyeastfbh1 AT mdshahjahanpsiddique multipleregulationofrad51mediatedhomologousrecombinationbyfissionyeastfbh1 AT yumikokawano multipleregulationofrad51mediatedhomologousrecombinationbyfissionyeastfbh1 AT fumiakiyamao multipleregulationofrad51mediatedhomologousrecombinationbyfissionyeastfbh1 AT hiroshiiwasaki multipleregulationofrad51mediatedhomologousrecombinationbyfissionyeastfbh1
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Multiple regulation of Rad51-mediated homologous recombination by fission yeast Fbh1.

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