A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance

Hidekatsu Yanai1,2, Yoshiharu Tomono3, Kumie Ito1,2, Yuji Hirowatari4, Hiroshi Yoshida1,5, Norio Tada1,21Department of Internal Medicine, 2Institute of Clinical Medicine and Research, 3Department of Nutrition, 5Department of Laboratory Medicine, Jikei University School of Medicine, chiba, Japan; 4Bi...

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Autores principales:	Hidekatsu Yanai, Yoshiharu Tomono, Kumie Ito, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/62080e3cfd094c22b988c066d3e668af
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spelling	oai:doaj.org-article:62080e3cfd094c22b988c066d3e668af2021-12-02T03:13:00ZA molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance1178-7007https://doaj.org/article/62080e3cfd094c22b988c066d3e668af2009-12-01T00:00:00Zhttp://www.dovepress.com/a-molecular-mechanism-for-diacylglycerol-mediated-promotion-of-negativ-a3852https://doaj.org/toc/1178-7007Hidekatsu Yanai1,2, Yoshiharu Tomono3, Kumie Ito1,2, Yuji Hirowatari4, Hiroshi Yoshida1,5, Norio Tada1,21Department of Internal Medicine, 2Institute of Clinical Medicine and Research, 3Department of Nutrition, 5Department of Laboratory Medicine, Jikei University School of Medicine, chiba, Japan; 4Bioscience Division, Tosoh Corporation, Kanagawa, JapanAims: A substitution of diacylglycerol (DAG) oil for triacylglycerol (TAG) oil in diet has been reported to reduce body fat and body weight, possibly by increasing postprandial energy expenditure (EE). We have previously studied plasma serotonin, which increases EE and exists in the small intestine, in individuals who ingested TAG and DAG oil, and found that DAG ingestion elevates plasma serotonin levels by about 50% compared with TAG ingestion. We studied the molecular mechanisms for DAG-mediated increase in serotonin and EE.Methods: We studied effects of 1-monoacylglycerol and 2-monoacylglycerol, distinct digestive products of DAG and TAG, respectively, on serotonin release from the Caco-2 cells (the human intestinal cell line, n = 8). Further, we studied effects of 1- and 2-monoacylglycerol, and serotonin on expression of mRNA associated with &beta;-oxidation, FA metabolism, and thermogenesis, in the Caco-2 cells (n = 5).Results: 1-monoacylglycerol (100 &micro;M 1-monooleyl glycerol [1-MOG]) significantly increased serotonin release from the Caco-2 cells compared with 2-monoacylglycerol (100 &micro;M 2-MOG) by 36.6%. Expression of mRNA of acyl-CoA oxidase (ACO), fatty acid translocase (FAT), and uncoupling protein-2 (UCP-2) were significantly higher in 100 &micro;M 1-MOG-treated Caco-2 cells than 100 &micro;M 2-MOG-treated cells by 12.8%, 23.7%, and 35.1%, respectively. Further, expression of mRNA of ACO, medium-chain acyl-CoA dehydrogenase, FAT, and UCP-2 were significantly elevated in serotonin (400 nM)-treated Caco-2 cells compared with cells incubated without serotonin by 28.7%, 30.1%, and 39.2%, respectively.Conclusions: Our study demonstrated that 1-monoacylglycerol, a digestive product of DAG, increases serotonin release from the Caco-2 cells, and enhances expression of genes associated with &beta;-oxidation, FA metabolism, and thermogenesis, and that serotonin increases expression of these genes, proposing a novel molecular mechanism for DAG-mediated promotion of negative caloric balance.Keywords: diacylglycerol, energy expenditure, intestine, serotonin, triacylglycerol Hidekatsu YanaiYoshiharu TomonoKumie Itoet alDove Medical PressarticleSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol 2010, Iss default, Pp 1-6 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Specialties of internal medicine RC581-951
spellingShingle	Specialties of internal medicine RC581-951 Hidekatsu Yanai Yoshiharu Tomono Kumie Ito et al A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
description	Hidekatsu Yanai1,2, Yoshiharu Tomono3, Kumie Ito1,2, Yuji Hirowatari4, Hiroshi Yoshida1,5, Norio Tada1,21Department of Internal Medicine, 2Institute of Clinical Medicine and Research, 3Department of Nutrition, 5Department of Laboratory Medicine, Jikei University School of Medicine, chiba, Japan; 4Bioscience Division, Tosoh Corporation, Kanagawa, JapanAims: A substitution of diacylglycerol (DAG) oil for triacylglycerol (TAG) oil in diet has been reported to reduce body fat and body weight, possibly by increasing postprandial energy expenditure (EE). We have previously studied plasma serotonin, which increases EE and exists in the small intestine, in individuals who ingested TAG and DAG oil, and found that DAG ingestion elevates plasma serotonin levels by about 50% compared with TAG ingestion. We studied the molecular mechanisms for DAG-mediated increase in serotonin and EE.Methods: We studied effects of 1-monoacylglycerol and 2-monoacylglycerol, distinct digestive products of DAG and TAG, respectively, on serotonin release from the Caco-2 cells (the human intestinal cell line, n = 8). Further, we studied effects of 1- and 2-monoacylglycerol, and serotonin on expression of mRNA associated with &beta;-oxidation, FA metabolism, and thermogenesis, in the Caco-2 cells (n = 5).Results: 1-monoacylglycerol (100 &micro;M 1-monooleyl glycerol [1-MOG]) significantly increased serotonin release from the Caco-2 cells compared with 2-monoacylglycerol (100 &micro;M 2-MOG) by 36.6%. Expression of mRNA of acyl-CoA oxidase (ACO), fatty acid translocase (FAT), and uncoupling protein-2 (UCP-2) were significantly higher in 100 &micro;M 1-MOG-treated Caco-2 cells than 100 &micro;M 2-MOG-treated cells by 12.8%, 23.7%, and 35.1%, respectively. Further, expression of mRNA of ACO, medium-chain acyl-CoA dehydrogenase, FAT, and UCP-2 were significantly elevated in serotonin (400 nM)-treated Caco-2 cells compared with cells incubated without serotonin by 28.7%, 30.1%, and 39.2%, respectively.Conclusions: Our study demonstrated that 1-monoacylglycerol, a digestive product of DAG, increases serotonin release from the Caco-2 cells, and enhances expression of genes associated with &beta;-oxidation, FA metabolism, and thermogenesis, and that serotonin increases expression of these genes, proposing a novel molecular mechanism for DAG-mediated promotion of negative caloric balance.Keywords: diacylglycerol, energy expenditure, intestine, serotonin, triacylglycerol
format	article
author	Hidekatsu Yanai Yoshiharu Tomono Kumie Ito et al
author_facet	Hidekatsu Yanai Yoshiharu Tomono Kumie Ito et al
author_sort	Hidekatsu Yanai
title	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_short	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_full	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_fullStr	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_full_unstemmed	A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
title_sort	molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/62080e3cfd094c22b988c066d3e668af
work_keys_str_mv	AT hidekatsuyanai amolecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT yoshiharutomono amolecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT kumieito amolecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT etal amolecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT hidekatsuyanai molecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT yoshiharutomono molecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT kumieito molecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance AT etal molecularmechanismfordiacylglycerolmediatedpromotionofnegativecaloricbalance
_version_	1718401856309297152

A molecular mechanism for diacylglycerol-mediated promotion of negative caloric balance

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