Faecal bacterial composition in horses with and without free faecal liquid: a case control study

Abstract Free faecal liquid (FFL) is a condition in horses which manifests as differential defecation of solid and liquid phases of faeces. The etiology of FFL is currently unknown, but deviances in the hindgut microbiota has been suggested to be of importance. The present study aimed to compare the...

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Autores principales:	Katrin M. Lindroth, Johan Dicksved, Erik Pelve, Viveca Båverud, Cecilia E. Müller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6209020a7f804ed993192ae530c5c82e
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spelling	oai:doaj.org-article:6209020a7f804ed993192ae530c5c82e2021-12-02T15:54:10ZFaecal bacterial composition in horses with and without free faecal liquid: a case control study10.1038/s41598-021-83897-42045-2322https://doaj.org/article/6209020a7f804ed993192ae530c5c82e2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83897-4https://doaj.org/toc/2045-2322Abstract Free faecal liquid (FFL) is a condition in horses which manifests as differential defecation of solid and liquid phases of faeces. The etiology of FFL is currently unknown, but deviances in the hindgut microbiota has been suggested to be of importance. The present study aimed to compare the faecal bacterial composition of farm-matched horses with (case, n = 50) and without (control, n = 50) FFL. Samples were collected at three different occasions. The V3 and V4 regions of the 16S rRNA gene were amplified and sequenced using Illumina sequencing. Also, samples were cultivated for detection of Clostridioides difficile and Clostridium perfringens. Analysis revealed similar faecal bacterial composition between case and control horses, but an effect of sampling period (p = 0.0001). Within sampling periods, 14 genera were present in higher or lower proportions in case compared to control horses in at least one sampling period. Compared to controls, case horses had higher relative abundance of Alloprevotella (adjusted p < 0.04) and lower relative abundance of Bacillus spp. (adjusted p < 0.03) in at least two sampling periods. All horses tested negative for C. difficile and C. perfringens by culture of faeces. Further studies are required to establish the clinical relevance of specific bacterial taxa in FFL.Katrin M. LindrothJohan DicksvedErik PelveViveca BåverudCecilia E. MüllerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katrin M. Lindroth Johan Dicksved Erik Pelve Viveca Båverud Cecilia E. Müller Faecal bacterial composition in horses with and without free faecal liquid: a case control study
description	Abstract Free faecal liquid (FFL) is a condition in horses which manifests as differential defecation of solid and liquid phases of faeces. The etiology of FFL is currently unknown, but deviances in the hindgut microbiota has been suggested to be of importance. The present study aimed to compare the faecal bacterial composition of farm-matched horses with (case, n = 50) and without (control, n = 50) FFL. Samples were collected at three different occasions. The V3 and V4 regions of the 16S rRNA gene were amplified and sequenced using Illumina sequencing. Also, samples were cultivated for detection of Clostridioides difficile and Clostridium perfringens. Analysis revealed similar faecal bacterial composition between case and control horses, but an effect of sampling period (p = 0.0001). Within sampling periods, 14 genera were present in higher or lower proportions in case compared to control horses in at least one sampling period. Compared to controls, case horses had higher relative abundance of Alloprevotella (adjusted p < 0.04) and lower relative abundance of Bacillus spp. (adjusted p < 0.03) in at least two sampling periods. All horses tested negative for C. difficile and C. perfringens by culture of faeces. Further studies are required to establish the clinical relevance of specific bacterial taxa in FFL.
format	article
author	Katrin M. Lindroth Johan Dicksved Erik Pelve Viveca Båverud Cecilia E. Müller
author_facet	Katrin M. Lindroth Johan Dicksved Erik Pelve Viveca Båverud Cecilia E. Müller
author_sort	Katrin M. Lindroth
title	Faecal bacterial composition in horses with and without free faecal liquid: a case control study
title_short	Faecal bacterial composition in horses with and without free faecal liquid: a case control study
title_full	Faecal bacterial composition in horses with and without free faecal liquid: a case control study
title_fullStr	Faecal bacterial composition in horses with and without free faecal liquid: a case control study
title_full_unstemmed	Faecal bacterial composition in horses with and without free faecal liquid: a case control study
title_sort	faecal bacterial composition in horses with and without free faecal liquid: a case control study
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6209020a7f804ed993192ae530c5c82e
work_keys_str_mv	AT katrinmlindroth faecalbacterialcompositioninhorseswithandwithoutfreefaecalliquidacasecontrolstudy AT johandicksved faecalbacterialcompositioninhorseswithandwithoutfreefaecalliquidacasecontrolstudy AT erikpelve faecalbacterialcompositioninhorseswithandwithoutfreefaecalliquidacasecontrolstudy AT vivecabaverud faecalbacterialcompositioninhorseswithandwithoutfreefaecalliquidacasecontrolstudy AT ceciliaemuller faecalbacterialcompositioninhorseswithandwithoutfreefaecalliquidacasecontrolstudy
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Faecal bacterial composition in horses with and without free faecal liquid: a case control study

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