Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.

Preterm births accounts for roughly 9% of all births worldwide and can have detrimental or even lethal consequences for the infant. However to develop new treatment that will lower the rate of preterm births, more knowledge is required on the factors contributing to the contraction and relaxation of...

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Autores principales:	Mette Rahbek, Sasan Nazemi, Lars Odum, Saurabh Gupta, Steen Seier Poulsen, Anders Hay-Schmidt, Dan Arne Klaerke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/620a1039295c4994bccae908ce93a4a3
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spelling	oai:doaj.org-article:620a1039295c4994bccae908ce93a4a32021-11-18T08:33:44ZExpression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.1932-620310.1371/journal.pone.0087652https://doaj.org/article/620a1039295c4994bccae908ce93a4a32014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24505302/?tool=EBIhttps://doaj.org/toc/1932-6203Preterm births accounts for roughly 9% of all births worldwide and can have detrimental or even lethal consequences for the infant. However to develop new treatment that will lower the rate of preterm births, more knowledge is required on the factors contributing to the contraction and relaxation of the myometrium. The small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) has been identified in the myometrium of several species including humans, mice and rats, but with great inter species variation of the expression pattern and regulation. The aim of this study was to investigate the expression of SK3 in the uterus of rats stimulated with 17β-estradiol and progesterone in order to get an in depth understanding of the rat uterine SK3. Using immunohistochemistry SK3 was localized to the glandular and luminal endometrial lamina epitheliali. Furthermore, a weak signal was observed in the myometrium. Using Western blot the protein level of SK3 was found to increase in uteri from animals treated with 17β-estradiol, an effect that was not reflected at the mRNA level. The levels of mRNA for SK3 were significantly lower in the uterus of 17β-estradiol-treated animals than in the uterus of ovariectomized animals. We conclude that the SK channels are present in the endometrial epithelium, and possibly also in the myometrium of the rat uterus. Furthermore, the hormonal effect on SK3 caused by 17β-estradiol includes divergent regulation at mRNA and protein levels.Mette RahbekSasan NazemiLars OdumSaurabh GuptaSteen Seier PoulsenAnders Hay-SchmidtDan Arne KlaerkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e87652 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mette Rahbek Sasan Nazemi Lars Odum Saurabh Gupta Steen Seier Poulsen Anders Hay-Schmidt Dan Arne Klaerke Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.
description	Preterm births accounts for roughly 9% of all births worldwide and can have detrimental or even lethal consequences for the infant. However to develop new treatment that will lower the rate of preterm births, more knowledge is required on the factors contributing to the contraction and relaxation of the myometrium. The small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) has been identified in the myometrium of several species including humans, mice and rats, but with great inter species variation of the expression pattern and regulation. The aim of this study was to investigate the expression of SK3 in the uterus of rats stimulated with 17β-estradiol and progesterone in order to get an in depth understanding of the rat uterine SK3. Using immunohistochemistry SK3 was localized to the glandular and luminal endometrial lamina epitheliali. Furthermore, a weak signal was observed in the myometrium. Using Western blot the protein level of SK3 was found to increase in uteri from animals treated with 17β-estradiol, an effect that was not reflected at the mRNA level. The levels of mRNA for SK3 were significantly lower in the uterus of 17β-estradiol-treated animals than in the uterus of ovariectomized animals. We conclude that the SK channels are present in the endometrial epithelium, and possibly also in the myometrium of the rat uterus. Furthermore, the hormonal effect on SK3 caused by 17β-estradiol includes divergent regulation at mRNA and protein levels.
format	article
author	Mette Rahbek Sasan Nazemi Lars Odum Saurabh Gupta Steen Seier Poulsen Anders Hay-Schmidt Dan Arne Klaerke
author_facet	Mette Rahbek Sasan Nazemi Lars Odum Saurabh Gupta Steen Seier Poulsen Anders Hay-Schmidt Dan Arne Klaerke
author_sort	Mette Rahbek
title	Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.
title_short	Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.
title_full	Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.
title_fullStr	Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.
title_full_unstemmed	Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.
title_sort	expression of the small conductance ca²⁺-activated potassium channel subtype 3 (sk3) in rat uterus after stimulation with 17β-estradiol.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/620a1039295c4994bccae908ce93a4a3
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Expression of the small conductance Ca²⁺-activated potassium channel subtype 3 (SK3) in rat uterus after stimulation with 17β-estradiol.

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