Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall

Abstract This work describes a coordinate and comprehensive view on the time course of the alterations occurring at the level of the cell wall during adaptation of a yeast cell population to sudden exposure to a sub-lethal stress induced by acetic acid. Acetic acid is a major inhibitory compound in...

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Autores principales:	Ricardo A. Ribeiro, Miguel V. Vitorino, Cláudia P. Godinho, Nuno Bourbon-Melo, Tiago T. Robalo, Fábio Fernandes, Mário S. Rodrigues, Isabel Sá-Correia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/620c704f2a7841198df8f19133f799ac
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spelling	oai:doaj.org-article:620c704f2a7841198df8f19133f799ac2021-12-02T17:41:27ZYeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall10.1038/s41598-021-92069-32045-2322https://doaj.org/article/620c704f2a7841198df8f19133f799ac2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92069-3https://doaj.org/toc/2045-2322Abstract This work describes a coordinate and comprehensive view on the time course of the alterations occurring at the level of the cell wall during adaptation of a yeast cell population to sudden exposure to a sub-lethal stress induced by acetic acid. Acetic acid is a major inhibitory compound in industrial bioprocesses and a widely used preservative in foods and beverages. Results indicate that yeast cell wall resistance to lyticase activity increases during acetic acid-induced growth latency, corresponding to yeast population adaptation to sudden exposure to this stress. This response correlates with: (i) increased cell stiffness, assessed by atomic force microscopy (AFM); (ii) increased content of cell wall β-glucans, assessed by fluorescence microscopy, and (iii) slight increase of the transcription level of the GAS1 gene encoding a β-1,3-glucanosyltransferase that leads to elongation of (1→3)-β-d-glucan chains. Collectively, results reinforce the notion that the adaptive yeast response to acetic acid stress involves a coordinate alteration of the cell wall at the biophysical and molecular levels. These alterations guarantee a robust adaptive response essential to limit the futile cycle associated to the re-entry of the toxic acid form after the active expulsion of acetate from the cell interior.Ricardo A. RibeiroMiguel V. VitorinoCláudia P. GodinhoNuno Bourbon-MeloTiago T. RobaloFábio FernandesMário S. RodriguesIsabel Sá-CorreiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ricardo A. Ribeiro Miguel V. Vitorino Cláudia P. Godinho Nuno Bourbon-Melo Tiago T. Robalo Fábio Fernandes Mário S. Rodrigues Isabel Sá-Correia Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
description	Abstract This work describes a coordinate and comprehensive view on the time course of the alterations occurring at the level of the cell wall during adaptation of a yeast cell population to sudden exposure to a sub-lethal stress induced by acetic acid. Acetic acid is a major inhibitory compound in industrial bioprocesses and a widely used preservative in foods and beverages. Results indicate that yeast cell wall resistance to lyticase activity increases during acetic acid-induced growth latency, corresponding to yeast population adaptation to sudden exposure to this stress. This response correlates with: (i) increased cell stiffness, assessed by atomic force microscopy (AFM); (ii) increased content of cell wall β-glucans, assessed by fluorescence microscopy, and (iii) slight increase of the transcription level of the GAS1 gene encoding a β-1,3-glucanosyltransferase that leads to elongation of (1→3)-β-d-glucan chains. Collectively, results reinforce the notion that the adaptive yeast response to acetic acid stress involves a coordinate alteration of the cell wall at the biophysical and molecular levels. These alterations guarantee a robust adaptive response essential to limit the futile cycle associated to the re-entry of the toxic acid form after the active expulsion of acetate from the cell interior.
format	article
author	Ricardo A. Ribeiro Miguel V. Vitorino Cláudia P. Godinho Nuno Bourbon-Melo Tiago T. Robalo Fábio Fernandes Mário S. Rodrigues Isabel Sá-Correia
author_facet	Ricardo A. Ribeiro Miguel V. Vitorino Cláudia P. Godinho Nuno Bourbon-Melo Tiago T. Robalo Fábio Fernandes Mário S. Rodrigues Isabel Sá-Correia
author_sort	Ricardo A. Ribeiro
title	Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
title_short	Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
title_full	Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
title_fullStr	Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
title_full_unstemmed	Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
title_sort	yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/620c704f2a7841198df8f19133f799ac
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Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall

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