Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits

Context: In China, the herb Sophora tonkinensis Gagnep. (Fabaceae, ST) (Committee of National Pharmacopeia. 2015) exhibits anti-inflammatory, antitumor, and antiviral effects. However, to date, there have been few studies on its gastrointestinal effect. Objective: The gastrointestinal effect of the...

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Autores principales:	Yangyou Li, Jing Li, Xin Liu, Jianwu Zhang, Xue Mei, Rudan Zheng, Wei Chen, Qian Zheng, Shangjie Zhong
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2019
Materias:	sophora tonkinensis antidiarrheal spasmolytic Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/620f0115bbeb4652a440f715795f2829
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spelling	oai:doaj.org-article:620f0115bbeb4652a440f715795f28292021-11-17T14:21:56ZAntidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits1388-02091744-511610.1080/13880209.2019.1645701https://doaj.org/article/620f0115bbeb4652a440f715795f28292019-01-01T00:00:00Zhttp://dx.doi.org/10.1080/13880209.2019.1645701https://doaj.org/toc/1388-0209https://doaj.org/toc/1744-5116Context: In China, the herb Sophora tonkinensis Gagnep. (Fabaceae, ST) (Committee of National Pharmacopeia. 2015) exhibits anti-inflammatory, antitumor, and antiviral effects. However, to date, there have been few studies on its gastrointestinal effect. Objective: The gastrointestinal effect of the methanol extract of ST rhizome (STR) was evaluated. Materials and methods: Study was conducted from February to December 2018. In vivo, antidiarrheal activity of STR (125, 250 and 500 mg/kg; orally) in castor oil-induced diarrheal mice was studied. In vitro, the effects of STR (0.01–10 mg/mL) on the isolated tissue preparations of rabbit jejunum were also investigated, the rabbit jejunum stripes were pre-contracted with Ach (10−5 M), K+ (60 mM) and tested in the presence of STR, the possible spasmolytic effect was analyzed in the pretreatment of the jejunum preparations with STR or verapamil in Ca2+-free high-K+ (60 mM) solution containing EDTA. Results: STR (125, 250 and 500 mg/kg) exhibited antidiarrheal activity. STR (0.01–10 mg/mL) completely relaxed spontaneously contracting, Ach (10−5 M) and high K+ (60 mM) induced contracted jejunum with an EC50 value of 0.66 (0.49–0.96), 0.39 (0.28–0.44) and 0.17 (0.10–0.21), similar to verapamil. Concentration–response curves of CaCl2 could be significantly moved to the right and down in the presence of STR (0.3, 1 mg/mL). Discussion and conclusions: Results suggest the presence of antidiarrheal activity and spasmolytic effects of STR, possibly mediated through Ca2+ channel blocking activity, providing the pharmacological basis for its traditional uses in gastrointestinal disorders.Yangyou LiJing LiXin LiuJianwu ZhangXue MeiRudan ZhengWei ChenQian ZhengShangjie ZhongTaylor & Francis Grouparticlesophora tonkinensisantidiarrhealspasmolyticTherapeutics. PharmacologyRM1-950ENPharmaceutical Biology, Vol 57, Iss 1, Pp 477-484 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	sophora tonkinensis antidiarrheal spasmolytic Therapeutics. Pharmacology RM1-950
spellingShingle	sophora tonkinensis antidiarrheal spasmolytic Therapeutics. Pharmacology RM1-950 Yangyou Li Jing Li Xin Liu Jianwu Zhang Xue Mei Rudan Zheng Wei Chen Qian Zheng Shangjie Zhong Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits
description	Context: In China, the herb Sophora tonkinensis Gagnep. (Fabaceae, ST) (Committee of National Pharmacopeia. 2015) exhibits anti-inflammatory, antitumor, and antiviral effects. However, to date, there have been few studies on its gastrointestinal effect. Objective: The gastrointestinal effect of the methanol extract of ST rhizome (STR) was evaluated. Materials and methods: Study was conducted from February to December 2018. In vivo, antidiarrheal activity of STR (125, 250 and 500 mg/kg; orally) in castor oil-induced diarrheal mice was studied. In vitro, the effects of STR (0.01–10 mg/mL) on the isolated tissue preparations of rabbit jejunum were also investigated, the rabbit jejunum stripes were pre-contracted with Ach (10−5 M), K+ (60 mM) and tested in the presence of STR, the possible spasmolytic effect was analyzed in the pretreatment of the jejunum preparations with STR or verapamil in Ca2+-free high-K+ (60 mM) solution containing EDTA. Results: STR (125, 250 and 500 mg/kg) exhibited antidiarrheal activity. STR (0.01–10 mg/mL) completely relaxed spontaneously contracting, Ach (10−5 M) and high K+ (60 mM) induced contracted jejunum with an EC50 value of 0.66 (0.49–0.96), 0.39 (0.28–0.44) and 0.17 (0.10–0.21), similar to verapamil. Concentration–response curves of CaCl2 could be significantly moved to the right and down in the presence of STR (0.3, 1 mg/mL). Discussion and conclusions: Results suggest the presence of antidiarrheal activity and spasmolytic effects of STR, possibly mediated through Ca2+ channel blocking activity, providing the pharmacological basis for its traditional uses in gastrointestinal disorders.
format	article
author	Yangyou Li Jing Li Xin Liu Jianwu Zhang Xue Mei Rudan Zheng Wei Chen Qian Zheng Shangjie Zhong
author_facet	Yangyou Li Jing Li Xin Liu Jianwu Zhang Xue Mei Rudan Zheng Wei Chen Qian Zheng Shangjie Zhong
author_sort	Yangyou Li
title	Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits
title_short	Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits
title_full	Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits
title_fullStr	Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits
title_full_unstemmed	Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits
title_sort	antidiarrheal activity of methanol extract of sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits
publisher	Taylor & Francis Group
publishDate	2019
url	https://doaj.org/article/620f0115bbeb4652a440f715795f2829
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Antidiarrheal activity of methanol extract of Sophora tonkinensis in mice and spasmolytic effect on smooth muscle contraction of isolated jejunum in rabbits

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