Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts

Abstract Serum amyloid A1 (SAA1) is an acute response protein, which is mainly produced by the liver, during infection. However, it remains unknown whether SAA1 can be produced in human fetal membranes where it is able to elicit events pertinent to labor initiation. We demonstrated that SAA1 was exp...

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Autores principales:	Wenjiao Li, Wangsheng Wang, Rujuan Zuo, Chao Liu, Qun Shu, Hao Ying, Kang Sun
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6221e152047c4d1dbea791c86caec8d4
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spelling	oai:doaj.org-article:6221e152047c4d1dbea791c86caec8d42021-12-02T11:53:02ZInduction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts10.1038/s41598-017-00782-92045-2322https://doaj.org/article/6221e152047c4d1dbea791c86caec8d42017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00782-9https://doaj.org/toc/2045-2322Abstract Serum amyloid A1 (SAA1) is an acute response protein, which is mainly produced by the liver, during infection. However, it remains unknown whether SAA1 can be produced in human fetal membranes where it is able to elicit events pertinent to labor initiation. We demonstrated that SAA1 was expressed in the fibroblasts and epithelium of the amnion and the trophoblasts of the chorion. Further study in human amnion fibroblasts showed that SAA1 production was augmented by interleukin-1β (IL-1β) and cortisol alone and synergistically, and SAA1 in turn induced the expression of IL-1β, interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and PGE2 production. These effects of SAA1 were mediated through activation of the NF-κB, p38 and ERK1/2 pathways via the toll-like receptor 4 (TLR4). Inhibition of TLR4 attenuated not only SAA1-induced activation of NF-κB, p38 and ERK1/2 but also increases in IL-1β, IL-6 and COX-2 expression. Moreover, SAA1 expression was increased in human amnion tissue following spontaneous labor. In conclusion, this study has demonstrated for the first time that SAA1 can be produced in human fetal membranes, which can be greatly induced in the presence of proinflammatory cytokines and glucocorticoids thereby producing effects associated with parturition.Wenjiao LiWangsheng WangRujuan ZuoChao LiuQun ShuHao YingKang SunNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenjiao Li Wangsheng Wang Rujuan Zuo Chao Liu Qun Shu Hao Ying Kang Sun Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts
description	Abstract Serum amyloid A1 (SAA1) is an acute response protein, which is mainly produced by the liver, during infection. However, it remains unknown whether SAA1 can be produced in human fetal membranes where it is able to elicit events pertinent to labor initiation. We demonstrated that SAA1 was expressed in the fibroblasts and epithelium of the amnion and the trophoblasts of the chorion. Further study in human amnion fibroblasts showed that SAA1 production was augmented by interleukin-1β (IL-1β) and cortisol alone and synergistically, and SAA1 in turn induced the expression of IL-1β, interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and PGE2 production. These effects of SAA1 were mediated through activation of the NF-κB, p38 and ERK1/2 pathways via the toll-like receptor 4 (TLR4). Inhibition of TLR4 attenuated not only SAA1-induced activation of NF-κB, p38 and ERK1/2 but also increases in IL-1β, IL-6 and COX-2 expression. Moreover, SAA1 expression was increased in human amnion tissue following spontaneous labor. In conclusion, this study has demonstrated for the first time that SAA1 can be produced in human fetal membranes, which can be greatly induced in the presence of proinflammatory cytokines and glucocorticoids thereby producing effects associated with parturition.
format	article
author	Wenjiao Li Wangsheng Wang Rujuan Zuo Chao Liu Qun Shu Hao Ying Kang Sun
author_facet	Wenjiao Li Wangsheng Wang Rujuan Zuo Chao Liu Qun Shu Hao Ying Kang Sun
author_sort	Wenjiao Li
title	Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts
title_short	Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts
title_full	Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts
title_fullStr	Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts
title_full_unstemmed	Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts
title_sort	induction of pro-inflammatory genes by serum amyloid a1 in human amnion fibroblasts
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/6221e152047c4d1dbea791c86caec8d4
work_keys_str_mv	AT wenjiaoli inductionofproinflammatorygenesbyserumamyloida1inhumanamnionfibroblasts AT wangshengwang inductionofproinflammatorygenesbyserumamyloida1inhumanamnionfibroblasts AT rujuanzuo inductionofproinflammatorygenesbyserumamyloida1inhumanamnionfibroblasts AT chaoliu inductionofproinflammatorygenesbyserumamyloida1inhumanamnionfibroblasts AT qunshu inductionofproinflammatorygenesbyserumamyloida1inhumanamnionfibroblasts AT haoying inductionofproinflammatorygenesbyserumamyloida1inhumanamnionfibroblasts AT kangsun inductionofproinflammatorygenesbyserumamyloida1inhumanamnionfibroblasts
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Induction of pro-inflammatory genes by serum amyloid A1 in human amnion fibroblasts

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