Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness

ABSTRACT Despite our extensive knowledge of the genetic regulation of heat shock proteins (HSPs), the evolutionary routes that allow bacteria to adaptively tune their HSP levels and corresponding proteostatic robustness have been explored less. In this report, directed evolution experiments using th...

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Autores principales:	Julien Mortier, Elisa Gayán, Ronald Van Eyken, Oscar Enrique Torres Montaguth, Ladan Khodaparast, Laleh Khodaparast, Bert Houben, Sebastien Carpentier, Frederic Rousseau, Joost Schymkowitz, Abram Aertsen
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	evolution genetics heat resistance protein aggregates Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/623a01e2ab7646c0bffe2fbedf477587
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spelling	oai:doaj.org-article:623a01e2ab7646c0bffe2fbedf4775872021-11-10T18:37:50ZGene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness10.1128/mBio.01129-212150-7511https://doaj.org/article/623a01e2ab7646c0bffe2fbedf4775872021-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01129-21https://doaj.org/toc/2150-7511ABSTRACT Despite our extensive knowledge of the genetic regulation of heat shock proteins (HSPs), the evolutionary routes that allow bacteria to adaptively tune their HSP levels and corresponding proteostatic robustness have been explored less. In this report, directed evolution experiments using the Escherichia coli model system unexpectedly revealed that seemingly random single mutations in its tnaA gene can confer significant heat resistance. Closer examination, however, indicated that these mutations create folding-deficient and aggregation-prone TnaA variants that in turn can endogenously and preemptively trigger HSP expression to cause heat resistance. These findings, importantly, demonstrate that even erosive mutations with disruptive effects on protein structure and functionality can still yield true gain-of-function alleles with a selective advantage in adaptive evolution.Julien MortierElisa GayánRonald Van EykenOscar Enrique Torres MontaguthLadan KhodaparastLaleh KhodaparastBert HoubenSebastien CarpentierFrederic RousseauJoost SchymkowitzAbram AertsenAmerican Society for Microbiologyarticleevolutiongeneticsheat resistanceprotein aggregatesMicrobiologyQR1-502ENmBio, Vol 12, Iss 4 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	evolution genetics heat resistance protein aggregates Microbiology QR1-502
spellingShingle	evolution genetics heat resistance protein aggregates Microbiology QR1-502 Julien Mortier Elisa Gayán Ronald Van Eyken Oscar Enrique Torres Montaguth Ladan Khodaparast Laleh Khodaparast Bert Houben Sebastien Carpentier Frederic Rousseau Joost Schymkowitz Abram Aertsen Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness
description	ABSTRACT Despite our extensive knowledge of the genetic regulation of heat shock proteins (HSPs), the evolutionary routes that allow bacteria to adaptively tune their HSP levels and corresponding proteostatic robustness have been explored less. In this report, directed evolution experiments using the Escherichia coli model system unexpectedly revealed that seemingly random single mutations in its tnaA gene can confer significant heat resistance. Closer examination, however, indicated that these mutations create folding-deficient and aggregation-prone TnaA variants that in turn can endogenously and preemptively trigger HSP expression to cause heat resistance. These findings, importantly, demonstrate that even erosive mutations with disruptive effects on protein structure and functionality can still yield true gain-of-function alleles with a selective advantage in adaptive evolution.
format	article
author	Julien Mortier Elisa Gayán Ronald Van Eyken Oscar Enrique Torres Montaguth Ladan Khodaparast Laleh Khodaparast Bert Houben Sebastien Carpentier Frederic Rousseau Joost Schymkowitz Abram Aertsen
author_facet	Julien Mortier Elisa Gayán Ronald Van Eyken Oscar Enrique Torres Montaguth Ladan Khodaparast Laleh Khodaparast Bert Houben Sebastien Carpentier Frederic Rousseau Joost Schymkowitz Abram Aertsen
author_sort	Julien Mortier
title	Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness
title_short	Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness
title_full	Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness
title_fullStr	Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness
title_full_unstemmed	Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness
title_sort	gene erosion can lead to gain-of-function alleles that contribute to bacterial fitness
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/623a01e2ab7646c0bffe2fbedf477587
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Gene Erosion Can Lead to Gain-of-Function Alleles That Contribute to Bacterial Fitness

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