Peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.

<h4>Background and aims</h4>Specific hyper-responsiveness towards an allergen and non-specific airway hyperreactivity both impair quality of life in patients with respiratory allergic diseases. We aimed to investigate cellular responses following specific and non-specific airway challeng...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Galateja Jordakieva, Julia Wallmann, René Schmutz, Patrick Lemell, Michael Wegmann, Thomas Nittke, Martina Mittlböck, Heinz Fehrenbach, Jasminka Godnic-Cvar, René Zieglmayer, Erika Jensen-Jarolim
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/623ce89613074b58bb24330d5307fd90
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:623ce89613074b58bb24330d5307fd90
record_format dspace
spelling oai:doaj.org-article:623ce89613074b58bb24330d5307fd902021-11-18T08:36:21ZPeripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.1932-620310.1371/journal.pone.0086701https://doaj.org/article/623ce89613074b58bb24330d5307fd902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466205/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and aims</h4>Specific hyper-responsiveness towards an allergen and non-specific airway hyperreactivity both impair quality of life in patients with respiratory allergic diseases. We aimed to investigate cellular responses following specific and non-specific airway challenges locally and systemically in i) sensitized BALB/c mice challenged with grass pollen allergen Phl p 5, and in ii) grass pollen sensitized allergic rhinitis subjects undergoing specific airway challenge in the Vienna Challenge Chamber (VCC).<h4>Methods and results</h4>BALB/c mice (n = 20) were intraperitoneally immunized with grass pollen allergen Phl p 5 and afterwards aerosol challenged with either the specific allergen Phl p 5 (n = 10) or the non-specific antigen ovalbumin (OVA) (n = 10). A protocol for inducing allergic asthma as well as allergic rhinitis, according to the united airway concept, was used. Both groups of exposed mice showed significantly reduced physical activity after airway challenge. Specific airway challenge further resulted in goblet cell hyperplasia, enhanced mucous secretion, intrapulmonary leukocyte infiltration and lymphoid follicle formation, associated with significant expression of IL-4, IL-5 and IL-13 in splenocytes and also partially in lung tissue. Concerning circulating blood cell dynamics, we observed a significant drop of erythrocyte counts, hemoglobin and hematocrit levels in both mouse groups, challenged with allergen or OVA. A significant decrease in circulating erythrocytes and hematocrit levels after airway challenges with grass pollen allergen was also found in grass pollen sensitized human rhinitis subjects (n = 42) at the VCC. The effects on peripheral leukocyte counts in mice and humans however were opposed, possibly due to the different primary inflammation sites.<h4>Conclusion</h4>Our data revealed that, besides significant leukocyte dynamics, particularly erythrocytes are involved in acute hypersensitivity reactions to respiratory allergens. A rapid recruitment of erythrocytes to the lungs to compensate for hypoxia is a possible explanation for these findings.Galateja JordakievaJulia WallmannRené SchmutzPatrick LemellMichael WegmannThomas NittkeMartina MittlböckHeinz FehrenbachJasminka Godnic-CvarRené ZieglmayerErika Jensen-JarolimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e86701 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Galateja Jordakieva
Julia Wallmann
René Schmutz
Patrick Lemell
Michael Wegmann
Thomas Nittke
Martina Mittlböck
Heinz Fehrenbach
Jasminka Godnic-Cvar
René Zieglmayer
Erika Jensen-Jarolim
Peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.
description <h4>Background and aims</h4>Specific hyper-responsiveness towards an allergen and non-specific airway hyperreactivity both impair quality of life in patients with respiratory allergic diseases. We aimed to investigate cellular responses following specific and non-specific airway challenges locally and systemically in i) sensitized BALB/c mice challenged with grass pollen allergen Phl p 5, and in ii) grass pollen sensitized allergic rhinitis subjects undergoing specific airway challenge in the Vienna Challenge Chamber (VCC).<h4>Methods and results</h4>BALB/c mice (n = 20) were intraperitoneally immunized with grass pollen allergen Phl p 5 and afterwards aerosol challenged with either the specific allergen Phl p 5 (n = 10) or the non-specific antigen ovalbumin (OVA) (n = 10). A protocol for inducing allergic asthma as well as allergic rhinitis, according to the united airway concept, was used. Both groups of exposed mice showed significantly reduced physical activity after airway challenge. Specific airway challenge further resulted in goblet cell hyperplasia, enhanced mucous secretion, intrapulmonary leukocyte infiltration and lymphoid follicle formation, associated with significant expression of IL-4, IL-5 and IL-13 in splenocytes and also partially in lung tissue. Concerning circulating blood cell dynamics, we observed a significant drop of erythrocyte counts, hemoglobin and hematocrit levels in both mouse groups, challenged with allergen or OVA. A significant decrease in circulating erythrocytes and hematocrit levels after airway challenges with grass pollen allergen was also found in grass pollen sensitized human rhinitis subjects (n = 42) at the VCC. The effects on peripheral leukocyte counts in mice and humans however were opposed, possibly due to the different primary inflammation sites.<h4>Conclusion</h4>Our data revealed that, besides significant leukocyte dynamics, particularly erythrocytes are involved in acute hypersensitivity reactions to respiratory allergens. A rapid recruitment of erythrocytes to the lungs to compensate for hypoxia is a possible explanation for these findings.
format article
author Galateja Jordakieva
Julia Wallmann
René Schmutz
Patrick Lemell
Michael Wegmann
Thomas Nittke
Martina Mittlböck
Heinz Fehrenbach
Jasminka Godnic-Cvar
René Zieglmayer
Erika Jensen-Jarolim
author_facet Galateja Jordakieva
Julia Wallmann
René Schmutz
Patrick Lemell
Michael Wegmann
Thomas Nittke
Martina Mittlböck
Heinz Fehrenbach
Jasminka Godnic-Cvar
René Zieglmayer
Erika Jensen-Jarolim
author_sort Galateja Jordakieva
title Peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.
title_short Peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.
title_full Peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.
title_fullStr Peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.
title_full_unstemmed Peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.
title_sort peripheral erythrocytes decrease upon specific respiratory challenge with grass pollen allergen in sensitized mice and in human subjects.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/623ce89613074b58bb24330d5307fd90
work_keys_str_mv AT galatejajordakieva peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT juliawallmann peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT reneschmutz peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT patricklemell peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT michaelwegmann peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT thomasnittke peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT martinamittlbock peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT heinzfehrenbach peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT jasminkagodniccvar peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT renezieglmayer peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
AT erikajensenjarolim peripheralerythrocytesdecreaseuponspecificrespiratorychallengewithgrasspollenallergeninsensitizedmiceandinhumansubjects
_version_ 1718421563719548928