Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.

<h4>Background/objectives</h4>Molecular epidemiology is a powerful tool to decipher the dynamics of viral transmission, quasispecies temporal evolution and origins. Little is known about the pH1N1 molecular dynamics in general population. A prospective study (CoPanFlu-RUN) was carried ou...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hervé Pascalis, Sarah Temmam, David A Wilkinson, Najla Dsouli, Magali Turpin, Xavier de Lamballerie, Koussay Dellagi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6242fbb6611b479384a9c17c44dcc72b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:6242fbb6611b479384a9c17c44dcc72b
record_format	dspace
spelling	oai:doaj.org-article:6242fbb6611b479384a9c17c44dcc72b2021-11-18T07:07:32ZMolecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.1932-620310.1371/journal.pone.0043742https://doaj.org/article/6242fbb6611b479384a9c17c44dcc72b2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952752/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background/objectives</h4>Molecular epidemiology is a powerful tool to decipher the dynamics of viral transmission, quasispecies temporal evolution and origins. Little is known about the pH1N1 molecular dynamics in general population. A prospective study (CoPanFlu-RUN) was carried out in Reunion Island to characterize pH1N1 genetic variability and molecular evolution occurring in population during the pH1N1 Influenza pandemic in 2009.<h4>Methodology</h4>We directly amplified pH1N1 genomes from 28 different nasal swabs (26 individuals from 21 households). Fifteen strains were fully sequenced and 13 partially. This includes pairs of sequences from different members of 5 separate households; and two pairs from individuals, collected at different times. We assessed the molecular evolution of pH1N1 by genetic variability and phylogenetic analyses.<h4>Principal findings</h4>We found that i) Reunion pH1N1 sequences stemmed from global "clade 7" but shaped two phylogenetic sub-clades; ii) D239E mutation was identified in the hemagglutinin protein of all Reunion sequences, a mutation which has been associated elsewhere with mild-, upper-respiratory tract pH1N1 infecting strains; iii) Date estimates from molecular phylogenies predicted clade emergence some time before the first detection of pH1N1 by the epidemiological surveillance system; iv) Phylogenetic relatedness was observed between Reunion pH1N1 viruses and those from other countries in South-western Indian Ocean area; v) Quasispecies populations were observed within households and individuals of the cohort-study.<h4>Conclusions</h4>Surveillance and/or prevention systems presently based on Influenza virus sequence variation should take into account that the majority of studies of pH1N1 Influenza generate genetic data for the HA/NA viral segments obtained from hospitalized-patients, which is potentially non-representative of the overall viral diversity within whole populations. Our observations highlight the importance of collecting unbiased data at the community level and conducting whole genome analysis to accurately understand viral dynamics.Hervé PascalisSarah TemmamDavid A WilkinsonNajla DsouliMagali TurpinXavier de LamballerieKoussay DellagiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43742 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hervé Pascalis Sarah Temmam David A Wilkinson Najla Dsouli Magali Turpin Xavier de Lamballerie Koussay Dellagi Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.
description	<h4>Background/objectives</h4>Molecular epidemiology is a powerful tool to decipher the dynamics of viral transmission, quasispecies temporal evolution and origins. Little is known about the pH1N1 molecular dynamics in general population. A prospective study (CoPanFlu-RUN) was carried out in Reunion Island to characterize pH1N1 genetic variability and molecular evolution occurring in population during the pH1N1 Influenza pandemic in 2009.<h4>Methodology</h4>We directly amplified pH1N1 genomes from 28 different nasal swabs (26 individuals from 21 households). Fifteen strains were fully sequenced and 13 partially. This includes pairs of sequences from different members of 5 separate households; and two pairs from individuals, collected at different times. We assessed the molecular evolution of pH1N1 by genetic variability and phylogenetic analyses.<h4>Principal findings</h4>We found that i) Reunion pH1N1 sequences stemmed from global "clade 7" but shaped two phylogenetic sub-clades; ii) D239E mutation was identified in the hemagglutinin protein of all Reunion sequences, a mutation which has been associated elsewhere with mild-, upper-respiratory tract pH1N1 infecting strains; iii) Date estimates from molecular phylogenies predicted clade emergence some time before the first detection of pH1N1 by the epidemiological surveillance system; iv) Phylogenetic relatedness was observed between Reunion pH1N1 viruses and those from other countries in South-western Indian Ocean area; v) Quasispecies populations were observed within households and individuals of the cohort-study.<h4>Conclusions</h4>Surveillance and/or prevention systems presently based on Influenza virus sequence variation should take into account that the majority of studies of pH1N1 Influenza generate genetic data for the HA/NA viral segments obtained from hospitalized-patients, which is potentially non-representative of the overall viral diversity within whole populations. Our observations highlight the importance of collecting unbiased data at the community level and conducting whole genome analysis to accurately understand viral dynamics.
format	article
author	Hervé Pascalis Sarah Temmam David A Wilkinson Najla Dsouli Magali Turpin Xavier de Lamballerie Koussay Dellagi
author_facet	Hervé Pascalis Sarah Temmam David A Wilkinson Najla Dsouli Magali Turpin Xavier de Lamballerie Koussay Dellagi
author_sort	Hervé Pascalis
title	Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.
title_short	Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.
title_full	Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.
title_fullStr	Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.
title_full_unstemmed	Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.
title_sort	molecular evolutionary analysis of ph1n1 2009 influenza virus in reunion island, south west indian ocean region: a cohort study.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/6242fbb6611b479384a9c17c44dcc72b
work_keys_str_mv	AT hervepascalis molecularevolutionaryanalysisofph1n12009influenzavirusinreunionislandsouthwestindianoceanregionacohortstudy AT sarahtemmam molecularevolutionaryanalysisofph1n12009influenzavirusinreunionislandsouthwestindianoceanregionacohortstudy AT davidawilkinson molecularevolutionaryanalysisofph1n12009influenzavirusinreunionislandsouthwestindianoceanregionacohortstudy AT najladsouli molecularevolutionaryanalysisofph1n12009influenzavirusinreunionislandsouthwestindianoceanregionacohortstudy AT magaliturpin molecularevolutionaryanalysisofph1n12009influenzavirusinreunionislandsouthwestindianoceanregionacohortstudy AT xavierdelamballerie molecularevolutionaryanalysisofph1n12009influenzavirusinreunionislandsouthwestindianoceanregionacohortstudy AT koussaydellagi molecularevolutionaryanalysisofph1n12009influenzavirusinreunionislandsouthwestindianoceanregionacohortstudy
_version_	1718423918432223232

Molecular evolutionary analysis of pH1N1 2009 influenza virus in Reunion Island, South West Indian Ocean region: a cohort study.

Ejemplares similares