A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

Abstract Polymerase chain reaction (PCR) is a powerful tool for nucleic acid amplification and quantification. However, long thermocycling time is a major limitation of the commercial PCR devices in the point-of-care (POC). Herein, we have developed a rapid droplet-based photonic PCR (dpPCR) system,...

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Autores principales:	Abbas Jalili, Maryam Bagheri, Amir Shamloo, Amir Hossein Kazemipour Ashkezari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6247cbe07e454ca18d30a51136353cd8
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spelling	oai:doaj.org-article:6247cbe07e454ca18d30a51136353cd82021-12-05T12:12:48ZA plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR10.1038/s41598-021-02535-12045-2322https://doaj.org/article/6247cbe07e454ca18d30a51136353cd82021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02535-1https://doaj.org/toc/2045-2322Abstract Polymerase chain reaction (PCR) is a powerful tool for nucleic acid amplification and quantification. However, long thermocycling time is a major limitation of the commercial PCR devices in the point-of-care (POC). Herein, we have developed a rapid droplet-based photonic PCR (dpPCR) system, including a gold (Au) nanofilm-based microfluidic chip and a plasmonic photothermal cycler. The chip is fabricated by adding mineral oil to uncured polydimethylsiloxane (PDMS) to suppress droplet evaporation in PDMS microfluidic chips during PCR thermocycling. A PDMS to gold bonding technique using a double-sided adhesive tape is applied to enhance the bonding strength between the oil-added PDMS and the gold nanofilm. Moreover, the gold nanofilm excited by two light-emitting diodes (LEDs) from the top and bottom sides of the chip provides fast heating of the PCR sample to 230 °C within 100 s. Such a design enables 30 thermal cycles from 60 to 95 °C within 13 min with the average heating and cooling rates of 7.37 ± 0.27 °C/s and 1.91 ± 0.03 °C/s, respectively. The experimental results demonstrate successful PCR amplification of the alcohol oxidase (AOX) gene using the rapid plasmonic photothermal cycler and exhibit the great performance of the microfluidic chip for droplet-based PCR.Abbas JaliliMaryam BagheriAmir ShamlooAmir Hossein Kazemipour AshkezariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abbas Jalili Maryam Bagheri Amir Shamloo Amir Hossein Kazemipour Ashkezari A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
description	Abstract Polymerase chain reaction (PCR) is a powerful tool for nucleic acid amplification and quantification. However, long thermocycling time is a major limitation of the commercial PCR devices in the point-of-care (POC). Herein, we have developed a rapid droplet-based photonic PCR (dpPCR) system, including a gold (Au) nanofilm-based microfluidic chip and a plasmonic photothermal cycler. The chip is fabricated by adding mineral oil to uncured polydimethylsiloxane (PDMS) to suppress droplet evaporation in PDMS microfluidic chips during PCR thermocycling. A PDMS to gold bonding technique using a double-sided adhesive tape is applied to enhance the bonding strength between the oil-added PDMS and the gold nanofilm. Moreover, the gold nanofilm excited by two light-emitting diodes (LEDs) from the top and bottom sides of the chip provides fast heating of the PCR sample to 230 °C within 100 s. Such a design enables 30 thermal cycles from 60 to 95 °C within 13 min with the average heating and cooling rates of 7.37 ± 0.27 °C/s and 1.91 ± 0.03 °C/s, respectively. The experimental results demonstrate successful PCR amplification of the alcohol oxidase (AOX) gene using the rapid plasmonic photothermal cycler and exhibit the great performance of the microfluidic chip for droplet-based PCR.
format	article
author	Abbas Jalili Maryam Bagheri Amir Shamloo Amir Hossein Kazemipour Ashkezari
author_facet	Abbas Jalili Maryam Bagheri Amir Shamloo Amir Hossein Kazemipour Ashkezari
author_sort	Abbas Jalili
title	A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
title_short	A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
title_full	A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
title_fullStr	A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
title_full_unstemmed	A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR
title_sort	plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic pcr
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/6247cbe07e454ca18d30a51136353cd8
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A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR

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