The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation

Vertical vibration (VV) is a type of whole body vibration, which induces muscle contraction through vibration to improve muscle strength and bone density. However, the mechanism of VV on muscle cell myotube formation is still unclear. In the current study, we aim to clarify the mechanism involved in...

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Autores principales:	Yi-Hsiung Lin, Liang-Yin Chou, Hsin-Chiao Chou, Chung-Hwan Chen, Lin Kang, Tsung-Lin Cheng, Chau-Zen Wang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	vibration stathmin myotube formation myogenic regulatory factors PI3K/Akt Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/624b84af288f4a8e957d9095ad6182d5
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spelling	oai:doaj.org-article:624b84af288f4a8e957d9095ad6182d52021-11-25T16:52:19ZThe Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation10.3390/biom111115832218-273Xhttps://doaj.org/article/624b84af288f4a8e957d9095ad6182d52021-10-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1583https://doaj.org/toc/2218-273XVertical vibration (VV) is a type of whole body vibration, which induces muscle contraction through vibration to improve muscle strength and bone density. However, the mechanism of VV on muscle cell myotube formation is still unclear. In the current study, we aim to clarify the mechanism involved in VV’s stimulation of myotube formation. In order to identify the molecules regulated by VV, we performed proteomics analysis including 2D electrophoresis combined with MALDI-TOF/TOF Mass. Stathmin was identified as a high potential molecule responding to VV stimulation, and we found that under VV stimulation, the expression of stathmin gene and protein increased in a time-dependent manner. In addition, we also confirmed that the increase of stathmin stimulated by VV is mediated through the PI3K/Akt pathway. Furthermore, stathmin siRNA significantly down-regulated the expression of myogenic regulatory factor (MRF) MyoD, decorin, and type I collagen (Col-I), and down-regulated the cellular process regulators such as FGF7, TGFBr1 and PAK3. Taken together, our results confirm that under the stimulation of VV, PI3K/Akt and stathmin would be activated, as well as the up-regulation of MRFs, such as FGF7, TGFBr1 and PAK3 to initiate myogenesis. It also showed that the response of MRF to VV stimulation was significantly related to stathmin expression, which also confirmed the importance of stathmin in the entire myotube formation process. This study may provide evidence of stathmin as a biological indicator of VV to increase muscle strength.Yi-Hsiung LinLiang-Yin ChouHsin-Chiao ChouChung-Hwan ChenLin KangTsung-Lin ChengChau-Zen WangMDPI AGarticlevibrationstathminmyotube formationmyogenic regulatory factorsPI3K/AktMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1583, p 1583 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	vibration stathmin myotube formation myogenic regulatory factors PI3K/Akt Microbiology QR1-502
spellingShingle	vibration stathmin myotube formation myogenic regulatory factors PI3K/Akt Microbiology QR1-502 Yi-Hsiung Lin Liang-Yin Chou Hsin-Chiao Chou Chung-Hwan Chen Lin Kang Tsung-Lin Cheng Chau-Zen Wang The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation
description	Vertical vibration (VV) is a type of whole body vibration, which induces muscle contraction through vibration to improve muscle strength and bone density. However, the mechanism of VV on muscle cell myotube formation is still unclear. In the current study, we aim to clarify the mechanism involved in VV’s stimulation of myotube formation. In order to identify the molecules regulated by VV, we performed proteomics analysis including 2D electrophoresis combined with MALDI-TOF/TOF Mass. Stathmin was identified as a high potential molecule responding to VV stimulation, and we found that under VV stimulation, the expression of stathmin gene and protein increased in a time-dependent manner. In addition, we also confirmed that the increase of stathmin stimulated by VV is mediated through the PI3K/Akt pathway. Furthermore, stathmin siRNA significantly down-regulated the expression of myogenic regulatory factor (MRF) MyoD, decorin, and type I collagen (Col-I), and down-regulated the cellular process regulators such as FGF7, TGFBr1 and PAK3. Taken together, our results confirm that under the stimulation of VV, PI3K/Akt and stathmin would be activated, as well as the up-regulation of MRFs, such as FGF7, TGFBr1 and PAK3 to initiate myogenesis. It also showed that the response of MRF to VV stimulation was significantly related to stathmin expression, which also confirmed the importance of stathmin in the entire myotube formation process. This study may provide evidence of stathmin as a biological indicator of VV to increase muscle strength.
format	article
author	Yi-Hsiung Lin Liang-Yin Chou Hsin-Chiao Chou Chung-Hwan Chen Lin Kang Tsung-Lin Cheng Chau-Zen Wang
author_facet	Yi-Hsiung Lin Liang-Yin Chou Hsin-Chiao Chou Chung-Hwan Chen Lin Kang Tsung-Lin Cheng Chau-Zen Wang
author_sort	Yi-Hsiung Lin
title	The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation
title_short	The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation
title_full	The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation
title_fullStr	The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation
title_full_unstemmed	The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation
title_sort	essential role of stathmin in myoblast c2c12 for vertical vibration-induced myotube formation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/624b84af288f4a8e957d9095ad6182d5
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The Essential Role of Stathmin in Myoblast C2C12 for Vertical Vibration-Induced Myotube Formation

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