Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China

Bacterial community plays a key role in environmental and ecological processes of river ecosystems. Given the special climatic and geographical conditions, studying the compositional characteristics of microorganisms in highland rivers and the relationship between such microorganisms and water physi...

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Autores principales:	Qianqian Zhang, Zhenbing Wu, Juan Zhao, Guojie Wang, Jingwen Hao, Shuyi Wang, Yaoyao Lin, Hongtao Guan, Jinyong Zhang, Shenglong Jian, Aihua Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Huangshui River high-throughput sequencing bacterioplankton community structure environment factors PICRUSt2 analysis Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/624c83acd4c34308b2c2b6ef247fc5e0
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spelling	oai:doaj.org-article:624c83acd4c34308b2c2b6ef247fc5e02021-11-25T18:24:40ZComposition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China10.3390/microorganisms91122602076-2607https://doaj.org/article/624c83acd4c34308b2c2b6ef247fc5e02021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2260https://doaj.org/toc/2076-2607Bacterial community plays a key role in environmental and ecological processes of river ecosystems. Given the special climatic and geographical conditions, studying the compositional characteristics of microorganisms in highland rivers and the relationship between such microorganisms and water physicochemical factors is important for an in-depth understanding of microbial ecological mechanisms. In the present study, high-throughput sequencing was used to investigate and study the bacterioplankton community of the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau. The results showed that the Huangshui River had significantly lower alpha diversity than the plain rivers. Despite the similarity in their environmental conditions, the main taxonomic compositions of the bacterial communities were distinct between the Huangshui River and polar regions (the Arctic and Antarctica). Proteobacteria accounted for the largest proportion (30.79–99.98%) of all the sequences, followed by Firmicutes (0–49.38%). <i>Acidiphilium</i> was the most numerous genera, which accounted for 0.03–86.16% of the assigned 16S reads, followed by <i>Acidocella</i> (0–95.9%), both belonging to Alphaproteobacteria. The diverse taxa of potential pathogens, such as Acinetobacter, Pseudomonas, and Aeromonas, were also identified. A principal coordinates analysis, coupled with a canonical correspondence analysis, showed spatial variations in the bacterial community composition. The water physical properties (e.g., Cr<sup>6+</sup>, total phosphorus, and COD<sub>Mn</sub>); altitude; and land use (e.g., urban land cover and aquaculture) determined the distribution of the bacterioplankton composition. PICRUSt2 revealed that the overall functional profiles of the bacterial communities in different samples were similar, and our results suggested the potential health risks of water sources in this area. This work provided valuable insight into the composition of the plankton bacterial community and its relationship with the environmental factors in the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau and a theoretical foundation for ecological health management.Qianqian ZhangZhenbing WuJuan ZhaoGuojie WangJingwen HaoShuyi WangYaoyao LinHongtao GuanJinyong ZhangShenglong JianAihua LiMDPI AGarticleHuangshui Riverhigh-throughput sequencingbacterioplankton community structureenvironment factorsPICRUSt2 analysisBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2260, p 2260 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Huangshui River high-throughput sequencing bacterioplankton community structure environment factors PICRUSt2 analysis Biology (General) QH301-705.5
spellingShingle	Huangshui River high-throughput sequencing bacterioplankton community structure environment factors PICRUSt2 analysis Biology (General) QH301-705.5 Qianqian Zhang Zhenbing Wu Juan Zhao Guojie Wang Jingwen Hao Shuyi Wang Yaoyao Lin Hongtao Guan Jinyong Zhang Shenglong Jian Aihua Li Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China
description	Bacterial community plays a key role in environmental and ecological processes of river ecosystems. Given the special climatic and geographical conditions, studying the compositional characteristics of microorganisms in highland rivers and the relationship between such microorganisms and water physicochemical factors is important for an in-depth understanding of microbial ecological mechanisms. In the present study, high-throughput sequencing was used to investigate and study the bacterioplankton community of the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau. The results showed that the Huangshui River had significantly lower alpha diversity than the plain rivers. Despite the similarity in their environmental conditions, the main taxonomic compositions of the bacterial communities were distinct between the Huangshui River and polar regions (the Arctic and Antarctica). Proteobacteria accounted for the largest proportion (30.79–99.98%) of all the sequences, followed by Firmicutes (0–49.38%). <i>Acidiphilium</i> was the most numerous genera, which accounted for 0.03–86.16% of the assigned 16S reads, followed by <i>Acidocella</i> (0–95.9%), both belonging to Alphaproteobacteria. The diverse taxa of potential pathogens, such as Acinetobacter, Pseudomonas, and Aeromonas, were also identified. A principal coordinates analysis, coupled with a canonical correspondence analysis, showed spatial variations in the bacterial community composition. The water physical properties (e.g., Cr<sup>6+</sup>, total phosphorus, and COD<sub>Mn</sub>); altitude; and land use (e.g., urban land cover and aquaculture) determined the distribution of the bacterioplankton composition. PICRUSt2 revealed that the overall functional profiles of the bacterial communities in different samples were similar, and our results suggested the potential health risks of water sources in this area. This work provided valuable insight into the composition of the plankton bacterial community and its relationship with the environmental factors in the Huangshui River in the ecotone zone of the Qinghai Plateau and Loess Plateau and a theoretical foundation for ecological health management.
format	article
author	Qianqian Zhang Zhenbing Wu Juan Zhao Guojie Wang Jingwen Hao Shuyi Wang Yaoyao Lin Hongtao Guan Jinyong Zhang Shenglong Jian Aihua Li
author_facet	Qianqian Zhang Zhenbing Wu Juan Zhao Guojie Wang Jingwen Hao Shuyi Wang Yaoyao Lin Hongtao Guan Jinyong Zhang Shenglong Jian Aihua Li
author_sort	Qianqian Zhang
title	Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China
title_short	Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China
title_full	Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China
title_fullStr	Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China
title_full_unstemmed	Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China
title_sort	composition and functional characteristics and influencing factors of bacterioplankton community in the huangshui river, china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/624c83acd4c34308b2c2b6ef247fc5e0
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Composition and Functional Characteristics and Influencing Factors of Bacterioplankton Community in the Huangshui River, China

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