Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice

Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease and is associated with high morbidity and mortality. Pogostemon cablin (Blanco) Benth/Huo Xiang (HX) is a perennial herb with unique anti-oxidant and anti-inflammatory properties, and thus, can positively affe...

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Autores principales:	Yizhe Cui, Qiuju Wang, Renxu Chang, Ahmad Aboragah, Juan J. Loor, Chuang Xu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	network pharmacology Pogostemon cablin (Blanco) Benth mice AML12 cells multi-targets NAFLD Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/6256fc54d82242abac7e8f9ae26e1dac
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spelling	oai:doaj.org-article:6256fc54d82242abac7e8f9ae26e1dac2021-11-30T17:59:10ZNetwork Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice1663-981210.3389/fphar.2021.789430https://doaj.org/article/6256fc54d82242abac7e8f9ae26e1dac2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphar.2021.789430/fullhttps://doaj.org/toc/1663-9812Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease and is associated with high morbidity and mortality. Pogostemon cablin (Blanco) Benth/Huo Xiang (HX) is a perennial herb with unique anti-oxidant and anti-inflammatory properties, and thus, can positively affect liver function. In this study, we used network pharmacology to predict the potential mechanism of HX on NAFLD. Pharmacological experiments were used to verify the effect of HX on the functions of NAFLD. Network pharmacology identified nine components that interacted with 82 NAFLD-related targets, revealing four target genes: TNF, IL6, TP53, and AKT1. HX prevents the development and progression of NAFLD through different pathways and targets with quercetin-regulated lipid metabolism, anti-inflammatory, and anti-oxidant pathways playing an essential role in the treatment of NAFLD. Compared with feeding HFD, HX significantly attenuated lipid accumulation in vivo with mice and also in vitro with mouse liver cells. A high dose of HX decreased hepatocyte lipid accumulation and the abundance of SREBF1 and FASN. Validation experiments revealed that HX inhibited the activation of NF-κB/IκB signaling and decreased the release and levels of pro-inflammatory factors (TNF-α and IL-6). These data suggest that HX can attenuate abnormal lipid metabolic responses and enhance antioxidant mechanisms. Thus, the pharmacological effects from plants used in traditional Chinese medicine are achievde through a multi-level response.Yizhe CuiYizhe CuiQiuju WangRenxu ChangAhmad AboragahJuan J. LoorChuang XuChuang XuFrontiers Media S.A.articlenetwork pharmacologyPogostemon cablin (Blanco) BenthmiceAML12 cellsmulti-targetsNAFLDTherapeutics. PharmacologyRM1-950ENFrontiers in Pharmacology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	network pharmacology Pogostemon cablin (Blanco) Benth mice AML12 cells multi-targets NAFLD Therapeutics. Pharmacology RM1-950
spellingShingle	network pharmacology Pogostemon cablin (Blanco) Benth mice AML12 cells multi-targets NAFLD Therapeutics. Pharmacology RM1-950 Yizhe Cui Yizhe Cui Qiuju Wang Renxu Chang Ahmad Aboragah Juan J. Loor Chuang Xu Chuang Xu Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice
description	Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver disease and is associated with high morbidity and mortality. Pogostemon cablin (Blanco) Benth/Huo Xiang (HX) is a perennial herb with unique anti-oxidant and anti-inflammatory properties, and thus, can positively affect liver function. In this study, we used network pharmacology to predict the potential mechanism of HX on NAFLD. Pharmacological experiments were used to verify the effect of HX on the functions of NAFLD. Network pharmacology identified nine components that interacted with 82 NAFLD-related targets, revealing four target genes: TNF, IL6, TP53, and AKT1. HX prevents the development and progression of NAFLD through different pathways and targets with quercetin-regulated lipid metabolism, anti-inflammatory, and anti-oxidant pathways playing an essential role in the treatment of NAFLD. Compared with feeding HFD, HX significantly attenuated lipid accumulation in vivo with mice and also in vitro with mouse liver cells. A high dose of HX decreased hepatocyte lipid accumulation and the abundance of SREBF1 and FASN. Validation experiments revealed that HX inhibited the activation of NF-κB/IκB signaling and decreased the release and levels of pro-inflammatory factors (TNF-α and IL-6). These data suggest that HX can attenuate abnormal lipid metabolic responses and enhance antioxidant mechanisms. Thus, the pharmacological effects from plants used in traditional Chinese medicine are achievde through a multi-level response.
format	article
author	Yizhe Cui Yizhe Cui Qiuju Wang Renxu Chang Ahmad Aboragah Juan J. Loor Chuang Xu Chuang Xu
author_facet	Yizhe Cui Yizhe Cui Qiuju Wang Renxu Chang Ahmad Aboragah Juan J. Loor Chuang Xu Chuang Xu
author_sort	Yizhe Cui
title	Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice
title_short	Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice
title_full	Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice
title_fullStr	Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice
title_full_unstemmed	Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice
title_sort	network pharmacology-based analysis of pogostemon cablin (blanco) benth beneficial effects to alleviate nonalcoholic fatty liver disease in mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/6256fc54d82242abac7e8f9ae26e1dac
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Network Pharmacology-Based Analysis of Pogostemon cablin (Blanco) Benth Beneficial Effects to Alleviate Nonalcoholic Fatty Liver Disease in Mice

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