Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system
Abstract The milk and milk products from cows reared under grazing system are believed to be healthier and hence have high demand compared to milk from cows reared in the non-grazing system. However, the effect of grazing on milk metabolites, specifically lipids has not been fully understood. In thi...
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2021
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oai:doaj.org-article:6261de6585ac46859187ecfec5a50ea82021-12-02T14:06:19ZDifferences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system10.1038/s41598-021-82412-z2045-2322https://doaj.org/article/6261de6585ac46859187ecfec5a50ea82021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82412-zhttps://doaj.org/toc/2045-2322Abstract The milk and milk products from cows reared under grazing system are believed to be healthier and hence have high demand compared to milk from cows reared in the non-grazing system. However, the effect of grazing on milk metabolites, specifically lipids has not been fully understood. In this study, we used acetonitrile precipitation and methanol:chloroform methods for extracting the milk metabolites followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) run to identify the different metabolites between the milk of grazing and non-grazing early lactating Malnad Gidda cows. Various carbohydrates, amino acids, nucleosides and vitamin derivatives were found to be differentially abundant in grazing cows. A total of 35 metabolites were differentially regulated (fold change above 1.5) between the two groups. Tyrosyl-threonine, histidinyl-cysteine, 1-methyladenine, l-cysteine and selenocysteine showed fold change above 3 in grazing cows. The lipid profile of milk showed a lesser difference between grazing and non-grazing cows as compared to polar metabolites. To the best of our knowledge, this is the largest inventory of milk metabolomics data of an Indian cattle (Bos indicus) breed. We believe that our study would help to emerge a field of Nutri-metabolomics and veterinary omics research.M. AshokanKerekoppa P. RameshaSweta HallurGayathree KarthikkeyanEkta RanaN. AzharuddinS. Reshma RajS. JeyakumarA. KumaresanMukund A. KataktalwareD. N. DasT. S. Keshava PrasadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) |
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Medicine R Science Q M. Ashokan Kerekoppa P. Ramesha Sweta Hallur Gayathree Karthikkeyan Ekta Rana N. Azharuddin S. Reshma Raj S. Jeyakumar A. Kumaresan Mukund A. Kataktalware D. N. Das T. S. Keshava Prasad Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| description |
Abstract The milk and milk products from cows reared under grazing system are believed to be healthier and hence have high demand compared to milk from cows reared in the non-grazing system. However, the effect of grazing on milk metabolites, specifically lipids has not been fully understood. In this study, we used acetonitrile precipitation and methanol:chloroform methods for extracting the milk metabolites followed by liquid chromatography–tandem mass spectrometry (LC–MS/MS) run to identify the different metabolites between the milk of grazing and non-grazing early lactating Malnad Gidda cows. Various carbohydrates, amino acids, nucleosides and vitamin derivatives were found to be differentially abundant in grazing cows. A total of 35 metabolites were differentially regulated (fold change above 1.5) between the two groups. Tyrosyl-threonine, histidinyl-cysteine, 1-methyladenine, l-cysteine and selenocysteine showed fold change above 3 in grazing cows. The lipid profile of milk showed a lesser difference between grazing and non-grazing cows as compared to polar metabolites. To the best of our knowledge, this is the largest inventory of milk metabolomics data of an Indian cattle (Bos indicus) breed. We believe that our study would help to emerge a field of Nutri-metabolomics and veterinary omics research. |
| format |
article |
| author |
M. Ashokan Kerekoppa P. Ramesha Sweta Hallur Gayathree Karthikkeyan Ekta Rana N. Azharuddin S. Reshma Raj S. Jeyakumar A. Kumaresan Mukund A. Kataktalware D. N. Das T. S. Keshava Prasad |
| author_facet |
M. Ashokan Kerekoppa P. Ramesha Sweta Hallur Gayathree Karthikkeyan Ekta Rana N. Azharuddin S. Reshma Raj S. Jeyakumar A. Kumaresan Mukund A. Kataktalware D. N. Das T. S. Keshava Prasad |
| author_sort |
M. Ashokan |
| title |
Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_short |
Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_full |
Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_fullStr |
Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_full_unstemmed |
Differences in milk metabolites in Malnad Gidda (Bos indicus) cows reared under pasture-based feeding system |
| title_sort |
differences in milk metabolites in malnad gidda (bos indicus) cows reared under pasture-based feeding system |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/6261de6585ac46859187ecfec5a50ea8 |
| work_keys_str_mv |
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