The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens.
Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of D...
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oai:doaj.org-article:62628b0f63ab421496ab82cbfef7e2b82021-11-25T05:58:40ZThe mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens.1932-620310.1371/journal.pone.0108775https://doaj.org/article/62628b0f63ab421496ab82cbfef7e2b82014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0108775https://doaj.org/toc/1932-6203Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of DON on the development of necrotic enteritis in broiler chickens. An experimental Clostridium perfringens infection study revealed that DON, at a contamination level of 3,000 to 4,000 µg/kg feed, increased the percentage of birds with subclinical necrotic enteritis from 20±2.6% to 47±3.0% (P<0.001). DON significantly reduced the transepithelial electrical resistance in duodenal segments (P<0.001) and decreased duodenal villus height (P = 0.014) indicating intestinal barrier disruption and intestinal epithelial damage, respectively. This may lead to an increased permeability of the intestinal epithelium and decreased absorption of dietary proteins. Protein analysis of duodenal content indeed showed that DON contamination resulted in a significant increase in total protein concentration (P = 0.023). Furthermore, DON had no effect on in vitro growth, alpha toxin production and netB toxin transcription of Clostridium perfringens. In conclusion, feed contamination with DON at concentrations below the European maximum guidance level of 5,000 µg/kg feed, is a predisposing factor for the development of necrotic enteritis in broilers. These results are associated with a negative effect of DON on the intestinal barrier function and increased intestinal protein availability, which may stimulate growth and toxin production of Clostridium perfringens.Gunther AntonissenFilip Van ImmerseelFrank PasmansRichard DucatelleFreddy HaesebrouckLeen TimbermontMarc VerlindenGeert Paul Jules JanssensVenessa EeckhautMia EeckhoutSarah De SaegerSabine HessenbergerAn MartelSiska CroubelsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e108775 (2014) |
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Medicine R Science Q |
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Medicine R Science Q Gunther Antonissen Filip Van Immerseel Frank Pasmans Richard Ducatelle Freddy Haesebrouck Leen Timbermont Marc Verlinden Geert Paul Jules Janssens Venessa Eeckhaut Mia Eeckhout Sarah De Saeger Sabine Hessenberger An Martel Siska Croubels The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. |
| description |
Both mycotoxin contamination of feed and Clostridium perfringens-induced necrotic enteritis have an increasing global economic impact on poultry production. Especially the Fusarium mycotoxin deoxynivalenol (DON) is a common feed contaminant. This study aimed at examining the predisposing effect of DON on the development of necrotic enteritis in broiler chickens. An experimental Clostridium perfringens infection study revealed that DON, at a contamination level of 3,000 to 4,000 µg/kg feed, increased the percentage of birds with subclinical necrotic enteritis from 20±2.6% to 47±3.0% (P<0.001). DON significantly reduced the transepithelial electrical resistance in duodenal segments (P<0.001) and decreased duodenal villus height (P = 0.014) indicating intestinal barrier disruption and intestinal epithelial damage, respectively. This may lead to an increased permeability of the intestinal epithelium and decreased absorption of dietary proteins. Protein analysis of duodenal content indeed showed that DON contamination resulted in a significant increase in total protein concentration (P = 0.023). Furthermore, DON had no effect on in vitro growth, alpha toxin production and netB toxin transcription of Clostridium perfringens. In conclusion, feed contamination with DON at concentrations below the European maximum guidance level of 5,000 µg/kg feed, is a predisposing factor for the development of necrotic enteritis in broilers. These results are associated with a negative effect of DON on the intestinal barrier function and increased intestinal protein availability, which may stimulate growth and toxin production of Clostridium perfringens. |
| format |
article |
| author |
Gunther Antonissen Filip Van Immerseel Frank Pasmans Richard Ducatelle Freddy Haesebrouck Leen Timbermont Marc Verlinden Geert Paul Jules Janssens Venessa Eeckhaut Mia Eeckhout Sarah De Saeger Sabine Hessenberger An Martel Siska Croubels |
| author_facet |
Gunther Antonissen Filip Van Immerseel Frank Pasmans Richard Ducatelle Freddy Haesebrouck Leen Timbermont Marc Verlinden Geert Paul Jules Janssens Venessa Eeckhaut Mia Eeckhout Sarah De Saeger Sabine Hessenberger An Martel Siska Croubels |
| author_sort |
Gunther Antonissen |
| title |
The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. |
| title_short |
The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. |
| title_full |
The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. |
| title_fullStr |
The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. |
| title_full_unstemmed |
The mycotoxin deoxynivalenol predisposes for the development of Clostridium perfringens-induced necrotic enteritis in broiler chickens. |
| title_sort |
mycotoxin deoxynivalenol predisposes for the development of clostridium perfringens-induced necrotic enteritis in broiler chickens. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2014 |
| url |
https://doaj.org/article/62628b0f63ab421496ab82cbfef7e2b8 |
| work_keys_str_mv |
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