The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model

Abstract Introduction Patients with comorbidity of ischemic stroke (IS) and diabetes mellitus (DM) show poor neurological functional recovery, and ischemic postconditioning (IPOC) should be considered a powerful neuroprotective method for IS. However, whether it should be introduced for patients wit...

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Autores principales:	Ling Zhao, Shufen Tan, Qiwei Liao, Xia Li, Tingyu Ke, Shuqing Li
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	diabetes mellitus ischemic stroke neuroprotective effect RNA‐sequence analysis of postconditioning Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/6267497acf694106b963b3af5a7c18a5
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spelling	oai:doaj.org-article:6267497acf694106b963b3af5a7c18a52021-11-25T06:06:36ZThe neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model2162-327910.1002/brb3.2354https://doaj.org/article/6267497acf694106b963b3af5a7c18a52021-11-01T00:00:00Zhttps://doi.org/10.1002/brb3.2354https://doaj.org/toc/2162-3279Abstract Introduction Patients with comorbidity of ischemic stroke (IS) and diabetes mellitus (DM) show poor neurological functional recovery, and ischemic postconditioning (IPOC) should be considered a powerful neuroprotective method for IS. However, whether it should be introduced for patients with IS and DM remains controversial. This study established a DM with IS (DMIS) tree shrew model, which was intervened by IPOC to assess its neuroprotective effects and also to analyze the relevant mechanism by RNA‐sequence and bioinformatics analysis. Methods Fifty‐four tree shrews were randomly divided into a sham operation control group, a DMIS group, and an IPOC group (DMIS model), with 18 tree shrews per group. Triphenyl tetrazolium chloride (TTC), hematoxylin‐eosin (HE) staining, transmission electron microscopy (TEM), and RNA‐sequence analysis were performed to assess the IPOC effect. Results IPOC reduced infarct size and reduced nerve cell injury in IS tree shrews with DM. RNA‐seq analysis showed that IPOC significantly increased the expression of the homeobox protein SIX3, while downregulating the expression of HLA class II histocompatibility antigens DQ beta 1 chain, CAS1 domain‐containing protein 1, and cytokine receptor‐like factor 2. The most downregulated signaling pathways include the NF‐κB signaling pathway, TNF signaling pathway, and Fc gamma R‐mediated phagocytosis. Conclusions IPOCs have a neuroprotective effect in a DMIS animal model that reduces infarct size and nerve cell injury. This mechanism might be related to reducing inflammation and stress responses that decreases the activity of TNF and NF‐κB signaling pathways.Ling ZhaoShufen TanQiwei LiaoXia LiTingyu KeShuqing LiWileyarticlediabetes mellitusischemic strokeneuroprotective effectRNA‐sequence analysis of postconditioningNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENBrain and Behavior, Vol 11, Iss 11, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetes mellitus ischemic stroke neuroprotective effect RNA‐sequence analysis of postconditioning Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	diabetes mellitus ischemic stroke neuroprotective effect RNA‐sequence analysis of postconditioning Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Ling Zhao Shufen Tan Qiwei Liao Xia Li Tingyu Ke Shuqing Li The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model
description	Abstract Introduction Patients with comorbidity of ischemic stroke (IS) and diabetes mellitus (DM) show poor neurological functional recovery, and ischemic postconditioning (IPOC) should be considered a powerful neuroprotective method for IS. However, whether it should be introduced for patients with IS and DM remains controversial. This study established a DM with IS (DMIS) tree shrew model, which was intervened by IPOC to assess its neuroprotective effects and also to analyze the relevant mechanism by RNA‐sequence and bioinformatics analysis. Methods Fifty‐four tree shrews were randomly divided into a sham operation control group, a DMIS group, and an IPOC group (DMIS model), with 18 tree shrews per group. Triphenyl tetrazolium chloride (TTC), hematoxylin‐eosin (HE) staining, transmission electron microscopy (TEM), and RNA‐sequence analysis were performed to assess the IPOC effect. Results IPOC reduced infarct size and reduced nerve cell injury in IS tree shrews with DM. RNA‐seq analysis showed that IPOC significantly increased the expression of the homeobox protein SIX3, while downregulating the expression of HLA class II histocompatibility antigens DQ beta 1 chain, CAS1 domain‐containing protein 1, and cytokine receptor‐like factor 2. The most downregulated signaling pathways include the NF‐κB signaling pathway, TNF signaling pathway, and Fc gamma R‐mediated phagocytosis. Conclusions IPOCs have a neuroprotective effect in a DMIS animal model that reduces infarct size and nerve cell injury. This mechanism might be related to reducing inflammation and stress responses that decreases the activity of TNF and NF‐κB signaling pathways.
format	article
author	Ling Zhao Shufen Tan Qiwei Liao Xia Li Tingyu Ke Shuqing Li
author_facet	Ling Zhao Shufen Tan Qiwei Liao Xia Li Tingyu Ke Shuqing Li
author_sort	Ling Zhao
title	The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model
title_short	The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model
title_full	The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model
title_fullStr	The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model
title_full_unstemmed	The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model
title_sort	neuroprotective effect and rna‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/6267497acf694106b963b3af5a7c18a5
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The neuroprotective effect and RNA‐sequence analysis of postconditioning on the ischemic stroke with diabetes mellitus tree shrew model

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