The 5′UTR in human adenoviruses: leader diversity in late gene expression
Abstract Human adenoviruses (HAdVs) shut down host cellular cap-dependent mRNA translation while initiating the translation of viral late mRNAs in a cap-independent manner. HAdV 5′ untranslated regions (5′UTRs) are crucial for cap-independent initiation, and influence mRNA localization and stability...
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Nature Portfolio
2017
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oai:doaj.org-article:62689b862cdf49849eb21e631744d10e2021-12-02T12:32:24ZThe 5′UTR in human adenoviruses: leader diversity in late gene expression10.1038/s41598-017-00747-y2045-2322https://doaj.org/article/62689b862cdf49849eb21e631744d10e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00747-yhttps://doaj.org/toc/2045-2322Abstract Human adenoviruses (HAdVs) shut down host cellular cap-dependent mRNA translation while initiating the translation of viral late mRNAs in a cap-independent manner. HAdV 5′ untranslated regions (5′UTRs) are crucial for cap-independent initiation, and influence mRNA localization and stability. However, HAdV translational regulation remains relatively uncharacterized. The HAdV tripartite leader (TPL), composed of three introns (TPL 1–3), is critical to the translation of HAdV late mRNA. Herein, we annotated and analyzed 72 HAdV genotypes for the HAdV TPL and another previously described leader, the i-leader. Using HAdV species D, type 37 (HAdV-D37), we show by reverse transcription PCR and Sanger sequencing that mRNAs of the HAdV-D37 E3 transcription unit are spliced to the TPL. We also identified a polycistronic mRNA for RID-α and RID-β. Analysis of the i-leader revealed a potential open reading frame within the leader sequence and the termination of this potential protein in TPL3. A potential new leader embedded within the E3 region was also detected and tentatively named the j-leader. These results suggest an underappreciated complexity of post-transcriptional regulation, and the importance of HAdV 5′UTRs for precisely coordinated viral protein expression along the path from genotype to phenotype.Mirja RamkeJeong Yoon LeeDavid W. DyerDonald SetoJaya RajaiyaJames ChodoshNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q Mirja Ramke Jeong Yoon Lee David W. Dyer Donald Seto Jaya Rajaiya James Chodosh The 5′UTR in human adenoviruses: leader diversity in late gene expression |
| description |
Abstract Human adenoviruses (HAdVs) shut down host cellular cap-dependent mRNA translation while initiating the translation of viral late mRNAs in a cap-independent manner. HAdV 5′ untranslated regions (5′UTRs) are crucial for cap-independent initiation, and influence mRNA localization and stability. However, HAdV translational regulation remains relatively uncharacterized. The HAdV tripartite leader (TPL), composed of three introns (TPL 1–3), is critical to the translation of HAdV late mRNA. Herein, we annotated and analyzed 72 HAdV genotypes for the HAdV TPL and another previously described leader, the i-leader. Using HAdV species D, type 37 (HAdV-D37), we show by reverse transcription PCR and Sanger sequencing that mRNAs of the HAdV-D37 E3 transcription unit are spliced to the TPL. We also identified a polycistronic mRNA for RID-α and RID-β. Analysis of the i-leader revealed a potential open reading frame within the leader sequence and the termination of this potential protein in TPL3. A potential new leader embedded within the E3 region was also detected and tentatively named the j-leader. These results suggest an underappreciated complexity of post-transcriptional regulation, and the importance of HAdV 5′UTRs for precisely coordinated viral protein expression along the path from genotype to phenotype. |
| format |
article |
| author |
Mirja Ramke Jeong Yoon Lee David W. Dyer Donald Seto Jaya Rajaiya James Chodosh |
| author_facet |
Mirja Ramke Jeong Yoon Lee David W. Dyer Donald Seto Jaya Rajaiya James Chodosh |
| author_sort |
Mirja Ramke |
| title |
The 5′UTR in human adenoviruses: leader diversity in late gene expression |
| title_short |
The 5′UTR in human adenoviruses: leader diversity in late gene expression |
| title_full |
The 5′UTR in human adenoviruses: leader diversity in late gene expression |
| title_fullStr |
The 5′UTR in human adenoviruses: leader diversity in late gene expression |
| title_full_unstemmed |
The 5′UTR in human adenoviruses: leader diversity in late gene expression |
| title_sort |
5′utr in human adenoviruses: leader diversity in late gene expression |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/62689b862cdf49849eb21e631744d10e |
| work_keys_str_mv |
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