Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF

Abstract Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low surv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luís F. Martins, Rui O. Costa, Joana R. Pedro, Paulo Aguiar, Sofia C. Serra, Fabio G. Teixeira, Nuno Sousa, António J. Salgado, Ramiro D. Almeida
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/62693d5eb2d74dcdbefb84f699115137
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:62693d5eb2d74dcdbefb84f699115137
record_format	dspace
spelling	oai:doaj.org-article:62693d5eb2d74dcdbefb84f6991151372021-12-02T11:52:24ZMesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF10.1038/s41598-017-03592-12045-2322https://doaj.org/article/62693d5eb2d74dcdbefb84f6991151372017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03592-1https://doaj.org/toc/2045-2322Abstract Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low survival rate when transplanted into the central nervous system (CNS). In line with this, MSCs secretome which present on its composition a wide range of molecules (neurotrophins, cytokines) and microvesicles, can be a solution to surpass these problems. However, the effect of MSCs secretome in axonal elongation is poorly understood. In this study, we demonstrate that application of MSCs secretome to both rat cortical and hippocampal neurons induces an increase in axonal length. In addition, we show that this growth effect is axonal intrinsic with no contribution from the cell body. To further understand which are the molecules required for secretome-induced axonal outgrowth effect, we depleted brain-derived neurotrophic factor (BDNF) from the secretome. Our results show that in the absence of BDNF, secretome-induced axonal elongation effect is lost and that axons present a reduced axonal growth rate. Altogether, our results demonstrate that MSCs secretome is able to promote axonal outgrowth in CNS neurons and this effect is mediated by BDNF.Luís F. MartinsRui O. CostaJoana R. PedroPaulo AguiarSofia C. SerraFabio G. TeixeiraNuno SousaAntónio J. SalgadoRamiro D. AlmeidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luís F. Martins Rui O. Costa Joana R. Pedro Paulo Aguiar Sofia C. Serra Fabio G. Teixeira Nuno Sousa António J. Salgado Ramiro D. Almeida Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
description	Abstract Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low survival rate when transplanted into the central nervous system (CNS). In line with this, MSCs secretome which present on its composition a wide range of molecules (neurotrophins, cytokines) and microvesicles, can be a solution to surpass these problems. However, the effect of MSCs secretome in axonal elongation is poorly understood. In this study, we demonstrate that application of MSCs secretome to both rat cortical and hippocampal neurons induces an increase in axonal length. In addition, we show that this growth effect is axonal intrinsic with no contribution from the cell body. To further understand which are the molecules required for secretome-induced axonal outgrowth effect, we depleted brain-derived neurotrophic factor (BDNF) from the secretome. Our results show that in the absence of BDNF, secretome-induced axonal elongation effect is lost and that axons present a reduced axonal growth rate. Altogether, our results demonstrate that MSCs secretome is able to promote axonal outgrowth in CNS neurons and this effect is mediated by BDNF.
format	article
author	Luís F. Martins Rui O. Costa Joana R. Pedro Paulo Aguiar Sofia C. Serra Fabio G. Teixeira Nuno Sousa António J. Salgado Ramiro D. Almeida
author_facet	Luís F. Martins Rui O. Costa Joana R. Pedro Paulo Aguiar Sofia C. Serra Fabio G. Teixeira Nuno Sousa António J. Salgado Ramiro D. Almeida
author_sort	Luís F. Martins
title	Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
title_short	Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
title_full	Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
title_fullStr	Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
title_full_unstemmed	Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
title_sort	mesenchymal stem cells secretome-induced axonal outgrowth is mediated by bdnf
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/62693d5eb2d74dcdbefb84f699115137
work_keys_str_mv	AT luisfmartins mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT ruiocosta mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT joanarpedro mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT pauloaguiar mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT sofiacserra mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT fabiogteixeira mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT nunosousa mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT antoniojsalgado mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf AT ramirodalmeida mesenchymalstemcellssecretomeinducedaxonaloutgrowthismediatedbybdnf
_version_	1718395049267429376

Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF

Ejemplares similares