A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.

We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monit...

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Autores principales:	Ka-Ho Leung, Lihua Lu, Modi Wang, Tsun-Yin Mak, Daniel Shiu-Hin Chan, Fung-Kit Tang, Chung-Hang Leung, Hiu-Yee Kwan, Zhiling Yu, Dik-Lung Ma
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/626ae1ae74e349fab71a25991530a70b
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spelling	oai:doaj.org-article:626ae1ae74e349fab71a25991530a70b2021-11-18T08:49:28ZA label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.1932-620310.1371/journal.pone.0077021https://doaj.org/article/626ae1ae74e349fab71a25991530a70b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24204723/?tool=EBIhttps://doaj.org/toc/1932-6203We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM.Ka-Ho LeungLihua LuModi WangTsun-Yin MakDaniel Shiu-Hin ChanFung-Kit TangChung-Hang LeungHiu-Yee KwanZhiling YuDik-Lung MaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e77021 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ka-Ho Leung Lihua Lu Modi Wang Tsun-Yin Mak Daniel Shiu-Hin Chan Fung-Kit Tang Chung-Hang Leung Hiu-Yee Kwan Zhiling Yu Dik-Lung Ma A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
description	We report herein the G-quadruplex-selective property of a luminescent cyclometallated iridium(III) complex for the detection of adenosine-5'-triphosphate (ATP) in aqueous solution. The ATP-binding aptamer was employed as the ATP recognition unit, while the iridium(III) complex was used to monitor the formation of the G-quadruplex structure induced by ATP. The sensitivity and fold enhancement of the assay were higher than those of the previously reported assay using the organic dye crystal violet as a fluorescent probe. This label-free luminescent switch-on assay exhibits high sensitivity and selectivity towards ATP with a limit of detection of 2.5 µM.
format	article
author	Ka-Ho Leung Lihua Lu Modi Wang Tsun-Yin Mak Daniel Shiu-Hin Chan Fung-Kit Tang Chung-Hang Leung Hiu-Yee Kwan Zhiling Yu Dik-Lung Ma
author_facet	Ka-Ho Leung Lihua Lu Modi Wang Tsun-Yin Mak Daniel Shiu-Hin Chan Fung-Kit Tang Chung-Hang Leung Hiu-Yee Kwan Zhiling Yu Dik-Lung Ma
author_sort	Ka-Ho Leung
title	A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
title_short	A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
title_full	A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
title_fullStr	A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
title_full_unstemmed	A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.
title_sort	label-free luminescent switch-on assay for atp using a g-quadruplex-selective iridium(iii) complex.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/626ae1ae74e349fab71a25991530a70b
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A label-free luminescent switch-on assay for ATP using a G-quadruplex-selective iridium(III) complex.

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