Resistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards

Management of cherry leaf spot disease, caused by the fungus <i>Blumeriella jaapii</i>, with succinate dehydrogenase inhibitor (SDHI) fungicides has been ongoing in Michigan tart cherry orchards for the past 17 years. After boscalid-resistant <i>B. jaapii</i> were first isola...

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Autores principales: Jacqueline Gleason, Jingyu Peng, Tyre J. Proffer, Suzanne M. Slack, Cory A. Outwater, Nikki L. Rothwell, George W. Sundin
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Publicado: MDPI AG 2021
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spelling oai:doaj.org-article:626d82aed3d44700ad6ca5161b085bb12021-11-25T18:24:09ZResistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards10.3390/microorganisms91121982076-2607https://doaj.org/article/626d82aed3d44700ad6ca5161b085bb12021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2198https://doaj.org/toc/2076-2607Management of cherry leaf spot disease, caused by the fungus <i>Blumeriella jaapii</i>, with succinate dehydrogenase inhibitor (SDHI) fungicides has been ongoing in Michigan tart cherry orchards for the past 17 years. After boscalid-resistant <i>B. jaapii</i> were first isolated from commercial orchards in 2010, premixes of SDHI fungicides fluopyram or fluxapyroxad with a quinone outside inhibitor were registered in 2012. Here, we report widespread resistance to fluopyram (Fluo<sup>R</sup>), fluxapyroxad (Flux<sup>R</sup>), and boscalid (Bosc<sup>R</sup>) in commercial orchard populations of <i>B. jaapii</i> in Michigan from surveys conducted between 2016 and 2019. A total of 26% of 1610 isolates from the 2016–2017 surveys exhibited the fully-resistant Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> phenotype and only 7% were sensitive to all three SDHIs. Practical resistance to fluopyram and fluxapyroxad was detected in 29 of 35 and 14 of 35 commercial tart cherry orchards, respectively, in surveys conducted in 2018 and 2019. Sequencing of the SdhB, SdhC, and SdhD target genes from 22 isolates with varying resistance phenotypes showed that Bosc<sup>S</sup> Fluo<sup>R</sup> Flux<sup>S</sup> isolates harbored either an I262V substitution in SdhB or an S84L substitution in SdhC. Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> isolates harbored an N86S substitution in SdhC, or contained the N86S substitution with the additional I262V substitution in SdhB. One Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> isolate contained both the I262V substitution in SdhB and the S84L substitution in SdhC. These mutational analyses suggest that Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> isolates evolved from fully sensitive Bosc<sup>S</sup>, Fluo<sup>S</sup>, Flux<sup>S</sup> isolates in the population and not from boscalid-resistant isolates that were prevalent in the 2010–2012 time period.Jacqueline GleasonJingyu PengTyre J. ProfferSuzanne M. SlackCory A. OutwaterNikki L. RothwellGeorge W. SundinMDPI AGarticlecherry leaf spotsuccinate dehydrogenase inhibitor<i>SdhB</i><i>SdhC</i>Biology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2198, p 2198 (2021)
institution DOAJ
collection DOAJ
language EN
topic cherry leaf spot
succinate dehydrogenase inhibitor
<i>SdhB</i>
<i>SdhC</i>
Biology (General)
QH301-705.5
spellingShingle cherry leaf spot
succinate dehydrogenase inhibitor
<i>SdhB</i>
<i>SdhC</i>
Biology (General)
QH301-705.5
Jacqueline Gleason
Jingyu Peng
Tyre J. Proffer
Suzanne M. Slack
Cory A. Outwater
Nikki L. Rothwell
George W. Sundin
Resistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards
description Management of cherry leaf spot disease, caused by the fungus <i>Blumeriella jaapii</i>, with succinate dehydrogenase inhibitor (SDHI) fungicides has been ongoing in Michigan tart cherry orchards for the past 17 years. After boscalid-resistant <i>B. jaapii</i> were first isolated from commercial orchards in 2010, premixes of SDHI fungicides fluopyram or fluxapyroxad with a quinone outside inhibitor were registered in 2012. Here, we report widespread resistance to fluopyram (Fluo<sup>R</sup>), fluxapyroxad (Flux<sup>R</sup>), and boscalid (Bosc<sup>R</sup>) in commercial orchard populations of <i>B. jaapii</i> in Michigan from surveys conducted between 2016 and 2019. A total of 26% of 1610 isolates from the 2016–2017 surveys exhibited the fully-resistant Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> phenotype and only 7% were sensitive to all three SDHIs. Practical resistance to fluopyram and fluxapyroxad was detected in 29 of 35 and 14 of 35 commercial tart cherry orchards, respectively, in surveys conducted in 2018 and 2019. Sequencing of the SdhB, SdhC, and SdhD target genes from 22 isolates with varying resistance phenotypes showed that Bosc<sup>S</sup> Fluo<sup>R</sup> Flux<sup>S</sup> isolates harbored either an I262V substitution in SdhB or an S84L substitution in SdhC. Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> isolates harbored an N86S substitution in SdhC, or contained the N86S substitution with the additional I262V substitution in SdhB. One Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> isolate contained both the I262V substitution in SdhB and the S84L substitution in SdhC. These mutational analyses suggest that Bosc<sup>R</sup> Fluo<sup>R</sup> Flux<sup>R</sup> isolates evolved from fully sensitive Bosc<sup>S</sup>, Fluo<sup>S</sup>, Flux<sup>S</sup> isolates in the population and not from boscalid-resistant isolates that were prevalent in the 2010–2012 time period.
format article
author Jacqueline Gleason
Jingyu Peng
Tyre J. Proffer
Suzanne M. Slack
Cory A. Outwater
Nikki L. Rothwell
George W. Sundin
author_facet Jacqueline Gleason
Jingyu Peng
Tyre J. Proffer
Suzanne M. Slack
Cory A. Outwater
Nikki L. Rothwell
George W. Sundin
author_sort Jacqueline Gleason
title Resistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards
title_short Resistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards
title_full Resistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards
title_fullStr Resistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards
title_full_unstemmed Resistance to Boscalid, Fluopyram and Fluxapyroxad in <i>Blumeriella jaapii</i> from Michigan (U.S.A.): Molecular Characterization and Assessment of Practical Resistance in Commercial Cherry Orchards
title_sort resistance to boscalid, fluopyram and fluxapyroxad in <i>blumeriella jaapii</i> from michigan (u.s.a.): molecular characterization and assessment of practical resistance in commercial cherry orchards
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/626d82aed3d44700ad6ca5161b085bb1
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