In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis

Abstract The microbiota fulfils a key role in the training and function of the immune system, which contributes to the symbiosis between the host and complex microbial communities. In this study, we characterized the interplay between vaginal bacteria and local immune mediators during dysbiosis in s...

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Autores principales:	Giuseppina Campisciano, Nunzia Zanotta, Danilo Licastro, Francesco De Seta, Manola Comar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/6270806712114272ab8e84743b33b263
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spelling	oai:doaj.org-article:6270806712114272ab8e84743b33b2632021-12-02T15:07:45ZIn vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis10.1038/s41598-018-20649-x2045-2322https://doaj.org/article/6270806712114272ab8e84743b33b2632018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20649-xhttps://doaj.org/toc/2045-2322Abstract The microbiota fulfils a key role in the training and function of the immune system, which contributes to the symbiosis between the host and complex microbial communities. In this study, we characterized the interplay between vaginal bacteria and local immune mediators during dysbiosis in selected women of reproductive age who were grouped according to Nugent’s criteria. The abundance of Gardnerella vaginalis and Bifidobacterium breve was increased in the intermediate dysbiotic status, while the presence of a plethora of non-resident bacteria characterized the group with overt vaginosis. In response to these increases, the anti-inflammatory IL1ra and pro-inflammatory IL2 increased, while the embryo trophic factors FGFβ and GMCSF decreased compared to the healthy milieu. A specific pattern, including IL1α, IL1β, IL8, MIG, MIP1α and RANTES, distinguished the intermediate group from the vaginosis group, while IL5 and IL13, which are secreted by Th2 cells, were significantly associated with the perturbation of the commensals Lactobacilli, Gardnerella and Ureaplasma. Summarizing, we postulate that although the dysbiotic condition triggers a pro-inflammatory process, the presence of a steady state level of Th2 may influence clinical manifestations. These results raise clinically relevant questions regarding the use of vaginal immunological markers as efficacious tools to monitor microbial alterations.Giuseppina CampiscianoNunzia ZanottaDanilo LicastroFrancesco De SetaManola ComarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giuseppina Campisciano Nunzia Zanotta Danilo Licastro Francesco De Seta Manola Comar In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis
description	Abstract The microbiota fulfils a key role in the training and function of the immune system, which contributes to the symbiosis between the host and complex microbial communities. In this study, we characterized the interplay between vaginal bacteria and local immune mediators during dysbiosis in selected women of reproductive age who were grouped according to Nugent’s criteria. The abundance of Gardnerella vaginalis and Bifidobacterium breve was increased in the intermediate dysbiotic status, while the presence of a plethora of non-resident bacteria characterized the group with overt vaginosis. In response to these increases, the anti-inflammatory IL1ra and pro-inflammatory IL2 increased, while the embryo trophic factors FGFβ and GMCSF decreased compared to the healthy milieu. A specific pattern, including IL1α, IL1β, IL8, MIG, MIP1α and RANTES, distinguished the intermediate group from the vaginosis group, while IL5 and IL13, which are secreted by Th2 cells, were significantly associated with the perturbation of the commensals Lactobacilli, Gardnerella and Ureaplasma. Summarizing, we postulate that although the dysbiotic condition triggers a pro-inflammatory process, the presence of a steady state level of Th2 may influence clinical manifestations. These results raise clinically relevant questions regarding the use of vaginal immunological markers as efficacious tools to monitor microbial alterations.
format	article
author	Giuseppina Campisciano Nunzia Zanotta Danilo Licastro Francesco De Seta Manola Comar
author_facet	Giuseppina Campisciano Nunzia Zanotta Danilo Licastro Francesco De Seta Manola Comar
author_sort	Giuseppina Campisciano
title	In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis
title_short	In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis
title_full	In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis
title_fullStr	In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis
title_full_unstemmed	In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis
title_sort	in vivo microbiome and associated immune markers: new insights into the pathogenesis of vaginal dysbiosis
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/6270806712114272ab8e84743b33b263
work_keys_str_mv	AT giuseppinacampisciano invivomicrobiomeandassociatedimmunemarkersnewinsightsintothepathogenesisofvaginaldysbiosis AT nunziazanotta invivomicrobiomeandassociatedimmunemarkersnewinsightsintothepathogenesisofvaginaldysbiosis AT danilolicastro invivomicrobiomeandassociatedimmunemarkersnewinsightsintothepathogenesisofvaginaldysbiosis AT francescodeseta invivomicrobiomeandassociatedimmunemarkersnewinsightsintothepathogenesisofvaginaldysbiosis AT manolacomar invivomicrobiomeandassociatedimmunemarkersnewinsightsintothepathogenesisofvaginaldysbiosis
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In vivo microbiome and associated immune markers: New insights into the pathogenesis of vaginal dysbiosis

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