The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues

Background: Among venomous elapid snakes, cobras have the highest public awareness, as their venom represents a combination of proteins, peptides, and enzymes that have a range of biochemical and pharmacological roles and are also the main constitutes of biological activity and lethal toxicity. O...

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Autores principales:	Asmaa Saad Mahmoud Shokhba, Mohamed A. Abdel-Rahman, Mohammed Alaa El-Deen A. Omran, Nahla Soliman El-Shenawy
Formato:	article
Lenguaje:	EN
Publicado:	Hamadan University of Medical Sciences 2021
Materias:	naja nubiae snake venom vessel permeability evans blue Medical technology R855-855.5
Acceso en línea:	https://doaj.org/article/627101797433411dadda9dde105457f9
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spelling	oai:doaj.org-article:627101797433411dadda9dde105457f92021-11-24T06:31:05ZThe Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues10.34172/ajmb.2021.042345-4113https://doaj.org/article/627101797433411dadda9dde105457f92021-06-01T00:00:00Zhttp://ajmb.umsha.ac.ir/PDF/ajmb-9-22.pdfhttps://doaj.org/toc/2345-4113Background: Among venomous elapid snakes, cobras have the highest public awareness, as their venom represents a combination of proteins, peptides, and enzymes that have a range of biochemical and pharmacological roles and are also the main constitutes of biological activity and lethal toxicity. Objectives: The study aimed to evaluate the effect of the venom of Egyptian Spitting Cobra, Naja nubiae, on the vascular permeability based on the extravasation of the azo dye Evans blue (EB) into the tissues of the liver and kidneys of animals envenomed with low (¼ LD50; 0.32 mg/kg) and high (½ LD50; 0.65 mg/ kg) doses at three sampling times (30, 120, 360 min) post-injection of the venom. Methods: Fifty-four adult male Albino rats (8 weeks old and 180±2 0 g body weight) were divided into three main groups (n=6). In the control group, rats were subcutaneously (SC) injected with saline solution. Envenomed groups were SC injected, one group with 0.32 mg/kg and the other group with 0.65 mg/kg body weight of crude venom, respectively. Rats were I.V injected with EB dye 20 minutes before SC injection with saline solution as control animals and with Naja nubiae venom as treatment groups. Results: The results illustrated a high significant rate of EB extravasation to hepatic and renal tissues by the colorimetric determination of EB dye concentration. Conclusion: The venom of Naja nubiae can cause increased hepatic and renal vascular permeability which may explain the inflammatory effect induced by this venom.Asmaa Saad Mahmoud ShokhbaMohamed A. Abdel-RahmanMohammed Alaa El-Deen A. OmranNahla Soliman El-ShenawyHamadan University of Medical Sciencesarticlenaja nubiaesnakevenomvessel permeabilityevans blueMedical technologyR855-855.5ENAvicenna Journal of Medical Biochemistry, Vol 9, Iss 1, Pp 22-25 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	naja nubiae snake venom vessel permeability evans blue Medical technology R855-855.5
spellingShingle	naja nubiae snake venom vessel permeability evans blue Medical technology R855-855.5 Asmaa Saad Mahmoud Shokhba Mohamed A. Abdel-Rahman Mohammed Alaa El-Deen A. Omran Nahla Soliman El-Shenawy The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues
description	Background: Among venomous elapid snakes, cobras have the highest public awareness, as their venom represents a combination of proteins, peptides, and enzymes that have a range of biochemical and pharmacological roles and are also the main constitutes of biological activity and lethal toxicity. Objectives: The study aimed to evaluate the effect of the venom of Egyptian Spitting Cobra, Naja nubiae, on the vascular permeability based on the extravasation of the azo dye Evans blue (EB) into the tissues of the liver and kidneys of animals envenomed with low (¼ LD50; 0.32 mg/kg) and high (½ LD50; 0.65 mg/ kg) doses at three sampling times (30, 120, 360 min) post-injection of the venom. Methods: Fifty-four adult male Albino rats (8 weeks old and 180±2 0 g body weight) were divided into three main groups (n=6). In the control group, rats were subcutaneously (SC) injected with saline solution. Envenomed groups were SC injected, one group with 0.32 mg/kg and the other group with 0.65 mg/kg body weight of crude venom, respectively. Rats were I.V injected with EB dye 20 minutes before SC injection with saline solution as control animals and with Naja nubiae venom as treatment groups. Results: The results illustrated a high significant rate of EB extravasation to hepatic and renal tissues by the colorimetric determination of EB dye concentration. Conclusion: The venom of Naja nubiae can cause increased hepatic and renal vascular permeability which may explain the inflammatory effect induced by this venom.
format	article
author	Asmaa Saad Mahmoud Shokhba Mohamed A. Abdel-Rahman Mohammed Alaa El-Deen A. Omran Nahla Soliman El-Shenawy
author_facet	Asmaa Saad Mahmoud Shokhba Mohamed A. Abdel-Rahman Mohammed Alaa El-Deen A. Omran Nahla Soliman El-Shenawy
author_sort	Asmaa Saad Mahmoud Shokhba
title	The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues
title_short	The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues
title_full	The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues
title_fullStr	The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues
title_full_unstemmed	The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues
title_sort	effect of venom of egyptian spitting cobra naja nubiae on vascular permeability of hepatic and renal tissues
publisher	Hamadan University of Medical Sciences
publishDate	2021
url	https://doaj.org/article/627101797433411dadda9dde105457f9
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The Effect of Venom of Egyptian Spitting Cobra Naja nubiae on Vascular Permeability of Hepatic and Renal Tissues

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