miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R

Glioma is a type of common intracranial tumor. In this study, we investigated the molecular mechanism by which miR-378a-3p regulates cisplatin (CDDP) chemosensitivity in glioma cells via insulin-like growth factor 1 receptor (IGF1R). U251/CDDP cells were treated with CDDP and transfected with miR-37...

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Autores principales:	Wang Yunjiang, Du Jia
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	glioma mir-378a-3p insulin like growth factor 1 receptor cisplatin chemosensitivity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/627611fb791741e88f2d9c75e70c301f
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id	oai:doaj.org-article:627611fb791741e88f2d9c75e70c301f
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spelling	oai:doaj.org-article:627611fb791741e88f2d9c75e70c301f2021-12-05T14:10:42ZmiR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R2391-541210.1515/biol-2021-0117https://doaj.org/article/627611fb791741e88f2d9c75e70c301f2021-11-01T00:00:00Zhttps://doi.org/10.1515/biol-2021-0117https://doaj.org/toc/2391-5412Glioma is a type of common intracranial tumor. In this study, we investigated the molecular mechanism by which miR-378a-3p regulates cisplatin (CDDP) chemosensitivity in glioma cells via insulin-like growth factor 1 receptor (IGF1R). U251/CDDP cells were treated with CDDP and transfected with miR-378a-3p mimics, NC mimics, or pcDNA-IGF1R. qRT-PCR was used to measure the differential level of miR-378a-3p. CCK-8 assay was used to test cell proliferation, and flow cytometry was used to analyze apoptosis. The targeting relationship between miR-378a-3p and IGF1R was tested through a dual-luciferase reporter gene assay. In contrast to normal glial cells, the miR-378a-3p level decreased in human glioma U251 cells and had lower expression in U251/CDDP cells. Compared with the CDDP group, miR-378a-3p significantly caused the inhibition of U251/CDDP cell proliferation and enhanced apoptosis in the miR-378a-3p mimics + CDDP group. Another experiment confirmed that IGF1R was a target gene of miR-378a-3p, and overexpression of miR-378a-3p inhibited IGF1R expression. In addition, co-overexpression of miR-378a-3p and IGF1R induced the upregulation of the U251/CDDP cell proliferation and the inhibition of apoptosis in the miR-378a-3p mimics + pcDNA-IGF1R + CDDP group. This study confirmed that miR-378a-3p promoted the sensitivity of glioma cells to CDDP in glioma patients via targeting IGF1R to increase the therapeutic effect during chemotherapy.Wang YunjiangDu JiaDe Gruyterarticlegliomamir-378a-3pinsulin like growth factor 1 receptorcisplatinchemosensitivityBiology (General)QH301-705.5ENOpen Life Sciences, Vol 16, Iss 1, Pp 1175-1181 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioma mir-378a-3p insulin like growth factor 1 receptor cisplatin chemosensitivity Biology (General) QH301-705.5
spellingShingle	glioma mir-378a-3p insulin like growth factor 1 receptor cisplatin chemosensitivity Biology (General) QH301-705.5 Wang Yunjiang Du Jia miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
description	Glioma is a type of common intracranial tumor. In this study, we investigated the molecular mechanism by which miR-378a-3p regulates cisplatin (CDDP) chemosensitivity in glioma cells via insulin-like growth factor 1 receptor (IGF1R). U251/CDDP cells were treated with CDDP and transfected with miR-378a-3p mimics, NC mimics, or pcDNA-IGF1R. qRT-PCR was used to measure the differential level of miR-378a-3p. CCK-8 assay was used to test cell proliferation, and flow cytometry was used to analyze apoptosis. The targeting relationship between miR-378a-3p and IGF1R was tested through a dual-luciferase reporter gene assay. In contrast to normal glial cells, the miR-378a-3p level decreased in human glioma U251 cells and had lower expression in U251/CDDP cells. Compared with the CDDP group, miR-378a-3p significantly caused the inhibition of U251/CDDP cell proliferation and enhanced apoptosis in the miR-378a-3p mimics + CDDP group. Another experiment confirmed that IGF1R was a target gene of miR-378a-3p, and overexpression of miR-378a-3p inhibited IGF1R expression. In addition, co-overexpression of miR-378a-3p and IGF1R induced the upregulation of the U251/CDDP cell proliferation and the inhibition of apoptosis in the miR-378a-3p mimics + pcDNA-IGF1R + CDDP group. This study confirmed that miR-378a-3p promoted the sensitivity of glioma cells to CDDP in glioma patients via targeting IGF1R to increase the therapeutic effect during chemotherapy.
format	article
author	Wang Yunjiang Du Jia
author_facet	Wang Yunjiang Du Jia
author_sort	Wang Yunjiang
title	miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
title_short	miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
title_full	miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
title_fullStr	miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
title_full_unstemmed	miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R
title_sort	mir-378a-3p regulates glioma cell chemosensitivity to cisplatin through igf1r
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/627611fb791741e88f2d9c75e70c301f
work_keys_str_mv	AT wangyunjiang mir378a3pregulatesgliomacellchemosensitivitytocisplatinthroughigf1r AT dujia mir378a3pregulatesgliomacellchemosensitivitytocisplatinthroughigf1r
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miR-378a-3p regulates glioma cell chemosensitivity to cisplatin through IGF1R

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