An antibacterial coated polymer prevents biofilm formation and implant-associated infection

Abstract To prevent infections associated with medical implants, various antimicrobial silver-coated implant materials have been developed. However, these materials do not always provide consistent antibacterial effects in vivo despite having dramatic antibacterial effects in vitro, probably because...

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Autores principales:	Hiroko Ishihama, Ken Ishii, Shigenori Nagai, Hiroaki Kakinuma, Aya Sasaki, Kenji Yoshioka, Tetsuya Kuramoto, Yuta Shiono, Haruki Funao, Norihiro Isogai, Takashi Tsuji, Yasunori Okada, Shigeo Koyasu, Yoshiaki Toyama, Masaya Nakamura, Mamoru Aizawa, Morio Matsumoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/627a1a201109410eb4e5c9a65670a394
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spelling	oai:doaj.org-article:627a1a201109410eb4e5c9a65670a3942021-12-02T14:27:01ZAn antibacterial coated polymer prevents biofilm formation and implant-associated infection10.1038/s41598-021-82992-w2045-2322https://doaj.org/article/627a1a201109410eb4e5c9a65670a3942021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82992-whttps://doaj.org/toc/2045-2322Abstract To prevent infections associated with medical implants, various antimicrobial silver-coated implant materials have been developed. However, these materials do not always provide consistent antibacterial effects in vivo despite having dramatic antibacterial effects in vitro, probably because the antibacterial effects involve silver-ion-mediated reactive oxygen species generation. Additionally, the silver application process often requires extremely high temperatures, which damage non-metal implant materials. We recently developed a bacteria-resistant coating consisting of hydroxyapatite film on which ionic silver is immobilized via inositol hexaphosphate chelation, using a series of immersion and drying steps performed at low heat. Here we applied this coating to a polymer, polyetheretherketone (PEEK), and analyzed the properties and antibacterial activity of the coated polymer in vitro and in vivo. The ionic silver coating demonstrated significant bactericidal activity and prevented bacterial biofilm formation in vitro. Bio-imaging of a soft tissue infection mouse model in which a silver-coated PEEK plate was implanted revealed a dramatic absence of bacterial signals 10 days after inoculation. These animals also showed a strong reduction in histological features of infection, compared to the control animals. This innovative coating can be applied to complex structures for clinical use, and could prevent infections associated with a variety of plastic implants.Hiroko IshihamaKen IshiiShigenori NagaiHiroaki KakinumaAya SasakiKenji YoshiokaTetsuya KuramotoYuta ShionoHaruki FunaoNorihiro IsogaiTakashi TsujiYasunori OkadaShigeo KoyasuYoshiaki ToyamaMasaya NakamuraMamoru AizawaMorio MatsumotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiroko Ishihama Ken Ishii Shigenori Nagai Hiroaki Kakinuma Aya Sasaki Kenji Yoshioka Tetsuya Kuramoto Yuta Shiono Haruki Funao Norihiro Isogai Takashi Tsuji Yasunori Okada Shigeo Koyasu Yoshiaki Toyama Masaya Nakamura Mamoru Aizawa Morio Matsumoto An antibacterial coated polymer prevents biofilm formation and implant-associated infection
description	Abstract To prevent infections associated with medical implants, various antimicrobial silver-coated implant materials have been developed. However, these materials do not always provide consistent antibacterial effects in vivo despite having dramatic antibacterial effects in vitro, probably because the antibacterial effects involve silver-ion-mediated reactive oxygen species generation. Additionally, the silver application process often requires extremely high temperatures, which damage non-metal implant materials. We recently developed a bacteria-resistant coating consisting of hydroxyapatite film on which ionic silver is immobilized via inositol hexaphosphate chelation, using a series of immersion and drying steps performed at low heat. Here we applied this coating to a polymer, polyetheretherketone (PEEK), and analyzed the properties and antibacterial activity of the coated polymer in vitro and in vivo. The ionic silver coating demonstrated significant bactericidal activity and prevented bacterial biofilm formation in vitro. Bio-imaging of a soft tissue infection mouse model in which a silver-coated PEEK plate was implanted revealed a dramatic absence of bacterial signals 10 days after inoculation. These animals also showed a strong reduction in histological features of infection, compared to the control animals. This innovative coating can be applied to complex structures for clinical use, and could prevent infections associated with a variety of plastic implants.
format	article
author	Hiroko Ishihama Ken Ishii Shigenori Nagai Hiroaki Kakinuma Aya Sasaki Kenji Yoshioka Tetsuya Kuramoto Yuta Shiono Haruki Funao Norihiro Isogai Takashi Tsuji Yasunori Okada Shigeo Koyasu Yoshiaki Toyama Masaya Nakamura Mamoru Aizawa Morio Matsumoto
author_facet	Hiroko Ishihama Ken Ishii Shigenori Nagai Hiroaki Kakinuma Aya Sasaki Kenji Yoshioka Tetsuya Kuramoto Yuta Shiono Haruki Funao Norihiro Isogai Takashi Tsuji Yasunori Okada Shigeo Koyasu Yoshiaki Toyama Masaya Nakamura Mamoru Aizawa Morio Matsumoto
author_sort	Hiroko Ishihama
title	An antibacterial coated polymer prevents biofilm formation and implant-associated infection
title_short	An antibacterial coated polymer prevents biofilm formation and implant-associated infection
title_full	An antibacterial coated polymer prevents biofilm formation and implant-associated infection
title_fullStr	An antibacterial coated polymer prevents biofilm formation and implant-associated infection
title_full_unstemmed	An antibacterial coated polymer prevents biofilm formation and implant-associated infection
title_sort	antibacterial coated polymer prevents biofilm formation and implant-associated infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/627a1a201109410eb4e5c9a65670a394
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An antibacterial coated polymer prevents biofilm formation and implant-associated infection

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