Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line

Clustered DNA lesions (CDL) containing 5′,8-cyclo-2′-deoxypurines (cdPus) are an example of extensive abnormalities occurring in the DNA helix and may impede cellular repair processes. The changes in the efficiency of nuclear base excision repair (BER) were investigated using (a) two cell lines, one...

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Main Authors:	Julia Kaźmierczak-Barańska, Karolina Boguszewska, Michał Szewczuk, Bolesław T. Karwowski
Format:	article
Language:	EN
Published:	MDPI AG 2021
Subjects:	cdA BER BJ XPC DNA repair DNA damage Biology (General) QH301-705.5
Online Access:	https://doaj.org/article/627a88aa942e4e10814bd8a2f11ab0e4
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spelling	oai:doaj.org-article:627a88aa942e4e10814bd8a2f11ab0e42021-11-25T17:13:20ZEffects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line10.3390/cells101132542073-4409https://doaj.org/article/627a88aa942e4e10814bd8a2f11ab0e42021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4409/10/11/3254https://doaj.org/toc/2073-4409Clustered DNA lesions (CDL) containing 5′,8-cyclo-2′-deoxypurines (cdPus) are an example of extensive abnormalities occurring in the DNA helix and may impede cellular repair processes. The changes in the efficiency of nuclear base excision repair (BER) were investigated using (a) two cell lines, one of the normal skin fibroblasts as a reference (BJ) and the second from <i>Xeroderma pigmentosum</i> patients’ skin (XPC), and (b) synthetic oligonucleotides with single- and double-stranded CDL (containing 5′,8-cyclo-2′-deoxyadenosine (cdA) and the abasic (AP) site at various distances between lesions). The nuclear BER has been observed and the effect of both cdA isomers (5′<i>R</i> and 5′<i>S</i>) presence in the DNA was tested. CdPus affected the repair of the second lesion within the CDL. The BER system more efficiently processed damage in the vicinity of the ScdA isomer and changes located in the 3′-end direction for dsCDL and in the 5′-end direction for ssCDL. The presented study is the very first investigation of the repair processes of the CDL containing cdPu considering cells derived from a <i>Xeroderma pigmentosum</i> patient.Julia Kaźmierczak-BarańskaKarolina BoguszewskaMichał SzewczukBolesław T. KarwowskiMDPI AGarticlecdABERBJXPCDNA repairDNA damageBiology (General)QH301-705.5ENCells, Vol 10, Iss 3254, p 3254 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cdA BER BJ XPC DNA repair DNA damage Biology (General) QH301-705.5
spellingShingle	cdA BER BJ XPC DNA repair DNA damage Biology (General) QH301-705.5 Julia Kaźmierczak-Barańska Karolina Boguszewska Michał Szewczuk Bolesław T. Karwowski Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line
description	Clustered DNA lesions (CDL) containing 5′,8-cyclo-2′-deoxypurines (cdPus) are an example of extensive abnormalities occurring in the DNA helix and may impede cellular repair processes. The changes in the efficiency of nuclear base excision repair (BER) were investigated using (a) two cell lines, one of the normal skin fibroblasts as a reference (BJ) and the second from <i>Xeroderma pigmentosum</i> patients’ skin (XPC), and (b) synthetic oligonucleotides with single- and double-stranded CDL (containing 5′,8-cyclo-2′-deoxyadenosine (cdA) and the abasic (AP) site at various distances between lesions). The nuclear BER has been observed and the effect of both cdA isomers (5′<i>R</i> and 5′<i>S</i>) presence in the DNA was tested. CdPus affected the repair of the second lesion within the CDL. The BER system more efficiently processed damage in the vicinity of the ScdA isomer and changes located in the 3′-end direction for dsCDL and in the 5′-end direction for ssCDL. The presented study is the very first investigation of the repair processes of the CDL containing cdPu considering cells derived from a <i>Xeroderma pigmentosum</i> patient.
format	article
author	Julia Kaźmierczak-Barańska Karolina Boguszewska Michał Szewczuk Bolesław T. Karwowski
author_facet	Julia Kaźmierczak-Barańska Karolina Boguszewska Michał Szewczuk Bolesław T. Karwowski
author_sort	Julia Kaźmierczak-Barańska
title	Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line
title_short	Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line
title_full	Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line
title_fullStr	Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line
title_full_unstemmed	Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line
title_sort	effects of 5′,8′-cyclo-2′-deoxypurines on the base excision repair of clustered dna lesions in nuclear extracts of the xpc cell line
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/627a88aa942e4e10814bd8a2f11ab0e4
work_keys_str_mv	AT juliakazmierczakbaranska effectsof58cyclo2deoxypurinesonthebaseexcisionrepairofclustereddnalesionsinnuclearextractsofthexpccellline AT karolinaboguszewska effectsof58cyclo2deoxypurinesonthebaseexcisionrepairofclustereddnalesionsinnuclearextractsofthexpccellline AT michałszewczuk effectsof58cyclo2deoxypurinesonthebaseexcisionrepairofclustereddnalesionsinnuclearextractsofthexpccellline AT bolesławtkarwowski effectsof58cyclo2deoxypurinesonthebaseexcisionrepairofclustereddnalesionsinnuclearextractsofthexpccellline
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Effects of 5′,8′-Cyclo-2′-Deoxypurines on the Base Excision Repair of Clustered DNA Lesions in Nuclear Extracts of the XPC Cell Line

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