Direct visualization of virus removal process in hollow fiber membrane using an optical microscope

Abstract Virus removal filters developed for the decontamination of small viruses from biotherapeutic products are widely used in basic research and critical step for drug production due to their long-established quality and robust performance. A variety of imaging techniques have been employed to e...

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Autores principales:	Miku Ayano, Yoshiyuki Sawamura, Tomoko Hongo-Hirasaki, Takayuki Nishizaka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/627f16234fa24f7094e6dfc4866478dc
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spelling	oai:doaj.org-article:627f16234fa24f7094e6dfc4866478dc2021-12-02T14:01:25ZDirect visualization of virus removal process in hollow fiber membrane using an optical microscope10.1038/s41598-020-78637-z2045-2322https://doaj.org/article/627f16234fa24f7094e6dfc4866478dc2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78637-zhttps://doaj.org/toc/2045-2322Abstract Virus removal filters developed for the decontamination of small viruses from biotherapeutic products are widely used in basic research and critical step for drug production due to their long-established quality and robust performance. A variety of imaging techniques have been employed to elucidate the mechanism(s) by which viruses are effectively captured by filter membranes, but they are limited to ‘static’ imaging. Here, we propose a novel method for detailed monitoring of ‘dynamic process’ of virus capture; specifically, direct examination of biomolecules during filtration under an ultra-stable optical microscope. Samples were fluorescently labeled and infused into a single hollow fiber membrane comprising cuprammonium regenerated-cellulose (Planova 20N). While proteins were able to pass through the membrane, virus-like particles (VLP) accumulated stably in a defined region of the membrane. After injecting the small amount of sample into the fiber membrane, the real-time process of trapping VLP in the membrane was quantified beyond the diffraction limit. The method presented here serves as a preliminary basis for determining optimum filtration conditions, and provides new insights into the structure of novel fiber membranes.Miku AyanoYoshiyuki SawamuraTomoko Hongo-HirasakiTakayuki NishizakaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miku Ayano Yoshiyuki Sawamura Tomoko Hongo-Hirasaki Takayuki Nishizaka Direct visualization of virus removal process in hollow fiber membrane using an optical microscope
description	Abstract Virus removal filters developed for the decontamination of small viruses from biotherapeutic products are widely used in basic research and critical step for drug production due to their long-established quality and robust performance. A variety of imaging techniques have been employed to elucidate the mechanism(s) by which viruses are effectively captured by filter membranes, but they are limited to ‘static’ imaging. Here, we propose a novel method for detailed monitoring of ‘dynamic process’ of virus capture; specifically, direct examination of biomolecules during filtration under an ultra-stable optical microscope. Samples were fluorescently labeled and infused into a single hollow fiber membrane comprising cuprammonium regenerated-cellulose (Planova 20N). While proteins were able to pass through the membrane, virus-like particles (VLP) accumulated stably in a defined region of the membrane. After injecting the small amount of sample into the fiber membrane, the real-time process of trapping VLP in the membrane was quantified beyond the diffraction limit. The method presented here serves as a preliminary basis for determining optimum filtration conditions, and provides new insights into the structure of novel fiber membranes.
format	article
author	Miku Ayano Yoshiyuki Sawamura Tomoko Hongo-Hirasaki Takayuki Nishizaka
author_facet	Miku Ayano Yoshiyuki Sawamura Tomoko Hongo-Hirasaki Takayuki Nishizaka
author_sort	Miku Ayano
title	Direct visualization of virus removal process in hollow fiber membrane using an optical microscope
title_short	Direct visualization of virus removal process in hollow fiber membrane using an optical microscope
title_full	Direct visualization of virus removal process in hollow fiber membrane using an optical microscope
title_fullStr	Direct visualization of virus removal process in hollow fiber membrane using an optical microscope
title_full_unstemmed	Direct visualization of virus removal process in hollow fiber membrane using an optical microscope
title_sort	direct visualization of virus removal process in hollow fiber membrane using an optical microscope
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/627f16234fa24f7094e6dfc4866478dc
work_keys_str_mv	AT mikuayano directvisualizationofvirusremovalprocessinhollowfibermembraneusinganopticalmicroscope AT yoshiyukisawamura directvisualizationofvirusremovalprocessinhollowfibermembraneusinganopticalmicroscope AT tomokohongohirasaki directvisualizationofvirusremovalprocessinhollowfibermembraneusinganopticalmicroscope AT takayukinishizaka directvisualizationofvirusremovalprocessinhollowfibermembraneusinganopticalmicroscope
_version_	1718392143097102336

Direct visualization of virus removal process in hollow fiber membrane using an optical microscope

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