Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination

The harmful jellyfish Cyanea nozakii Kishinouye has frequently occurred on Korean coasts, and its blooms have caused serious ecological and economic damages. DNA sequences of the C. nozakii for molecular detection and discrimination are relatively scarce. In this study, we determined the complete se...

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Autores principales:	Buhari Lawan Muhammad, Yoseph Seo, Jang-Seu Ki
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	cyanea nozakii jellyfish phylogenetic analysis tandem repeat ribosomal dna Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/628a2c7ea0004f9a87760689cc8c6087
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spelling	oai:doaj.org-article:628a2c7ea0004f9a87760689cc8c60872021-11-26T11:19:48ZEvaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination1976-83542151-248510.1080/19768354.2021.2006309https://doaj.org/article/628a2c7ea0004f9a87760689cc8c60872021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/19768354.2021.2006309https://doaj.org/toc/1976-8354https://doaj.org/toc/2151-2485The harmful jellyfish Cyanea nozakii Kishinouye has frequently occurred on Korean coasts, and its blooms have caused serious ecological and economic damages. DNA sequences of the C. nozakii for molecular detection and discrimination are relatively scarce. In this study, we determined the complete sequence of a single unit of tandemly repeated ribosomal DNA (rDNA) of the Korean C. nozakii and characterized the molecular features of the rDNA. The complete rDNA contained 8,003 bp (48.4% GC) with the same gene arrangement (18S, ITS1, 5.8S, ITS2, 28S, and IGS) to the typical eukaryotes. Dot plot analysis showed that the coding regions (18S, 5.8S, and 28S) were highly conserved, while the non-coding regions (ITS1, ITS2, and IGS) were more variable and parsimony-informative. The IGS contained a putative transcription termination signal (poly(T) tract) and four repeats of block minisatellites. Phylogenetic analyses using 18S and 28S rDNA revealed well-resolved relationships of C. nozakii within the order Semaeostomeae, separating it from other Cyanea species. The complete rDNA sequence provides various options for the selection of jellyfish taxonomic markers and may be useful for discriminating between species of C. nozakii and phylogeny reconstruction with close relatives.Buhari Lawan MuhammadYoseph SeoJang-Seu KiTaylor & Francis Grouparticlecyanea nozakiijellyfishphylogenetic analysistandem repeatribosomal dnaMedicine (General)R5-920Biology (General)QH301-705.5ENAnimal Cells and Systems, Vol 0, Iss 0, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyanea nozakii jellyfish phylogenetic analysis tandem repeat ribosomal dna Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	cyanea nozakii jellyfish phylogenetic analysis tandem repeat ribosomal dna Medicine (General) R5-920 Biology (General) QH301-705.5 Buhari Lawan Muhammad Yoseph Seo Jang-Seu Ki Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination
description	The harmful jellyfish Cyanea nozakii Kishinouye has frequently occurred on Korean coasts, and its blooms have caused serious ecological and economic damages. DNA sequences of the C. nozakii for molecular detection and discrimination are relatively scarce. In this study, we determined the complete sequence of a single unit of tandemly repeated ribosomal DNA (rDNA) of the Korean C. nozakii and characterized the molecular features of the rDNA. The complete rDNA contained 8,003 bp (48.4% GC) with the same gene arrangement (18S, ITS1, 5.8S, ITS2, 28S, and IGS) to the typical eukaryotes. Dot plot analysis showed that the coding regions (18S, 5.8S, and 28S) were highly conserved, while the non-coding regions (ITS1, ITS2, and IGS) were more variable and parsimony-informative. The IGS contained a putative transcription termination signal (poly(T) tract) and four repeats of block minisatellites. Phylogenetic analyses using 18S and 28S rDNA revealed well-resolved relationships of C. nozakii within the order Semaeostomeae, separating it from other Cyanea species. The complete rDNA sequence provides various options for the selection of jellyfish taxonomic markers and may be useful for discriminating between species of C. nozakii and phylogeny reconstruction with close relatives.
format	article
author	Buhari Lawan Muhammad Yoseph Seo Jang-Seu Ki
author_facet	Buhari Lawan Muhammad Yoseph Seo Jang-Seu Ki
author_sort	Buhari Lawan Muhammad
title	Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination
title_short	Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination
title_full	Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination
title_fullStr	Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination
title_full_unstemmed	Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination
title_sort	evaluation of the complete nuclear rdna unit sequence of the jellyfish cyanea nozakii kishinouye (scyphozoa: semaeostomeae) for molecular discrimination
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/628a2c7ea0004f9a87760689cc8c6087
work_keys_str_mv	AT buharilawanmuhammad evaluationofthecompletenuclearrdnaunitsequenceofthejellyfishcyaneanozakiikishinouyescyphozoasemaeostomeaeformoleculardiscrimination AT yosephseo evaluationofthecompletenuclearrdnaunitsequenceofthejellyfishcyaneanozakiikishinouyescyphozoasemaeostomeaeformoleculardiscrimination AT jangseuki evaluationofthecompletenuclearrdnaunitsequenceofthejellyfishcyaneanozakiikishinouyescyphozoasemaeostomeaeformoleculardiscrimination
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Evaluation of the complete nuclear rDNA unit sequence of the jellyfish Cyanea nozakii Kishinouye (Scyphozoa: Semaeostomeae) for molecular discrimination

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